Candida chromogenic medium, detection kit and detection method

Abstract

The invention relates to candida chromogenic medium, a detection kit and a detection method, belonging to the technical field of microbial diagnosis, in particular to the cultivation and identification of yeast in medical microbiology specimens, drugs and medical equipment sanitary inspection samples, public health surveillance samples and food (including cosmetics) sanitary inspection samples. The chromogenic medium of the invention is composed of basic medium, mixed chromogenic substrate and bacteriostat, wherein the mixed chromogenic substrate consists of aminocaproic glucosidase and alkaline phosphatase substrate, and candida specific enzyme is added into the mixed chromogenic substrate. The detection kit of the invention consists of the chromogenic medium, identification paper A containing enzyme substrate 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-aminogalactose and identification paper B containing enzyme substrate 5-bromo-4-chloro-3-indolyl-beta-D- glycopyranoside. In the invention, the candida chromogenic medium and the detection kit have the advantages of low cost and simple configuration, and the method can be applied to the separation and identification of candida rapidly, simply and accurately.

Description

technical field [0001] The invention relates to the field of microbial diagnosis, especially the cultivation and identification of yeast in medical microbial specimens, hygienic inspection samples of medicines and medical devices, public health monitoring samples and food (including cosmetics) hygienic inspection samples, especially the isolation of Candida bacteria Culture and identification of important strains of the genus Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata, and identification of Candida albicans. Background technique [0002] Candida (Candida spp., also known as Candida) is a kind of fungus, which not only widely exists in nature, but also usually exists in the oral cavity, upper respiratory tract, intestinal tract and vagina of normal people. Cause disease, when the immune function or general defense of the body is reduced or the mutual restriction of normal flora is out of balance, the bacteria multiply and change the growth form ...

AI Technical Summary

Problems solved by technology

[0005] In terms of the culture medium for isolating and identifying Candida bacteria, Canadian patent CA2634325 describes the use of sweet potato extract powder and yeast extract powder as the main raw materials of the medium base, adding the chromogenic agent 5-bromo-4- Chloro-3-indolyl-N-acetyl-β-D-aminoglucopyranoside (5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucopyranoside or X-N-acetyl-β- D-glucopyranoside) and the fluorescent substrate 4-methylumbelliferone-N-acetyl-β-D-galactosamine (4MU-N-acetyl-β-D-galactosaminide), by observing the colony color (blue , yellow and white) and whether the colonies produce fluorescence to distinguish the main species of Candida, but on the one hand, the fluorescence cannot be directly observed by the naked eye, which is inconvenient; on the other hand, light yellow and milky white are not easy to distinguish, which will lead to the bias in judgment

Japanese patent JP2003310298, describes a 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside (X-gluc) substrate to distinguish Candida albicans and Candida tropicalis from other fungi developed, but cannot identify other species of the genus

Venitia M. Cooke (New Chromogenic Agar Medium for the Identification of Candida spp., Applied and Environmental Microbiology, Vol.68 (7): 3622-3627, 2002) has described a kind of based on Sa Paul's culture medium and added assay Candida albicans ( C. albicans) and C. dubliniensis (C. dubliniensis) chromogenic substrate for glucosaminidase activity (VLPA-GlcNAc) to identify the chromogenic medium of Candida spp. White colonies with red dots, other Candida species can only be classified as white or pink colonies and cannot be distinguished

A. Gaschet et al. (Evaluation of CandiSelect4, a new chromogenic medium for isolation and presumptive identification of Candidaspecies from clinical specimens, J. of Medical Mycology, V.18(2), 89-95, 2008) described a new isolation and presumptive identification of CandiSelect4, a chromogenic medium for identifying Candida bacteria in clinical specimens, has the same separation effect as CHROMAgar Candida (Komagar Company) on Candida, but neither of them can completely obtain Candida albicans, which is important for this genus. Satisfactory identification results (a small number of false positive results) cannot distinguish Candida dublin from Candida albicans, and the target bacteria can be distinguished by the naked eye after at least 48 hours of culture, and the best time is 72 hours

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