The invention belongs to the technical field of
electrochemical immunoassay, and relates to an
assembly array type multi-channel
capacitance immune sensor based on a glass capillary structure, which is formed through the following steps: fixing
antibody A (
antigen A) on the inner wall of a glass capillary through aminosilane
reagent and
glutaraldehyde, inserting a
platinum wire
electrode into the glass capillary, fixing the
platinum wire
electrode in the glass capillary, injecting nonspecific
protein, sealing active adsorption sites, washing,
drying, and combining two glass capillaries formed through the above steps, thereby obtaining an immune
electrode pair sensitive to A. Then an immune
electrode pair sensitive to B, an immune
electrode pair sensitive to C and an immune
electrode pair sensitive to D are formed in sequence in the identical method. The
assembly array type multi-channel
capacitance immune sensor can be obtained through assembling the electrode pairs. A corresponding
immune reaction substance is cultured and washed in the glass capillary and is then inserted in substrate solution with urinary
enzyme, and the urinary
enzyme labeled
antigen (urinary
enzyme labeled
antibody) catalyzes the reaction of the substrate, thereby increasing the
capacitance of the solution. Then the content of the
antigen (
antibody) can be measured.