60 results about "Shellfish poison" patented technology

The invention discloses a method for rapidly detecting shellfish toxin. The method comprises the following steps: (1) extracting and purifying shellfish toxin of a sample; (2) identifying the shellfish toxin; (3) preparing a shellfish toxin-BSA coupling antigen; (4) preparing and purifying a poisoning monoclonal antibody; (5) preparing immunomagnetic microspheres; (6) preparing a glucan-antibody-fluorescein mixed marker; (7) purifying the mixed marker; and (8) preparing a fluorescence immune chromatography test strip. The method is high in specificity and low in detection limit and can realize quantitative determination of antigen components.

The invention discloses a method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry. The method comprises the following steps: extracting a sample by use of an 80% methanol aqueous solution, mixing and diluting with a phosphate buffer solution, and performing immunoaffinity selective special purification, and performing liquid chromatography-tandem mass spectrometry. According to the use characteristics of a diarrhetic immunoaffinity column, parameters of sample liquid, eluate, eluent and the like are optimized; electrospray anions are ionized, a multi-reaction monitoring mode is adopted, and quantification based on an external standard method is achieved. The method is less in matrix interference, strong in purification effect, high in sensitivity and applicable to analysis measurement on diarrhetic shellfish poisons in shellfishes.

The invention discloses a diarrhetic shellfish poison sample high-flux preprocessing device. The device comprises a control circuit, a switch power supply, a motor speed regulation plate, a peristaltic pump, a power supply interface, power supply sockets, a communication interface, a liquid tube interface, corresponding connecting tubes and a cabinet, wherein the power supply interface is connected with the switch power supply to input commercial power in order to supply power for the device; the power supply sockets are connected to the control circuit, and supply power for independent working devices; the communication interface is connected to the control circuit; the motor speed regulation plate and the control circuit are fixedly arranged above the switch power supply, and are connected with corresponding peristaltic pumps to control the working of the peristaltic pumps; the peristaltic pumps are fixedly arranged on the cabinet through a pedestal; the inner side of the front panel liquid tube interface is connected with the peristaltic pumps through Teflon tubes, and the interface is fixed through a band; and the outer side of the liquid tube interface leads out liquid flow through a Teflon tube. The device realizes high-flux preprocessing of a shellfish meat sample in the diarrhetic shellfish poison detection process, and improves the sample preprocessing efficiency.

The invention relates to a method for preparing canned mactra veneriformis with primary taste, which comprises the steps of material selection, acceptance, selection, sand spitting, grading and selection, detection of mud clams, adding of concentrated clam soup and fine rice wine, vacuum sealing, sterilization and cooling, inspection under a constant temperature, boxing up and the like, specifically, selecting the mactra veneriformis of which heavy metal content, microbial indicators, saxitoxin and the like meeting relevant standards to carry out sand spitting and purification: wherein the water temperature is 20 DEG C to 30 DEG C, the salinity is 1.5% to 3.5%, and the time for spitting sand is 24 hours; then added 30g according to the proportion of 2:1 of the concentrated clam soup to the fine rice wine; packaging and sealing at a 700mm Hg vacuum level, wherein the sterilization formula is 5-5-5min / 125 DEG C; cooling down to a water temperature of 10 DEG C; and storing in a refrigerated warehouse after reaching commercial sterility through inspection. According to the method provided by the invention, the mactra veneriformis meat, not subjected to strong flavor conditioning but added with the special fine rice wine mixed with the primary taste soup, is processed into the special canned food with primary taste and has the advantages of simpleness in processing, convenience for eating and delicious taste, which provide a reference for promoting the utility value of mactra veneriformis and other low-value conches.