Capillary electrophoresis electrochemical enzyme linked immunoassay for detecting amnesic shellfish toxicity
A technology of capillary electrophoresis and shellfish toxins, which is applied in the direction of material analysis, material analysis, and measuring devices through electromagnetic means, can solve problems that have not been reported, and achieve the effects of less reagent consumption, high separation efficiency, and high sensitivity
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Embodiment 1
[0031] Example 1 Detection of Amnestic Shellfish Toxin in Standard Samples by Capillary Electrophoresis Electrochemical Enzyme-Linked Immunoassay
[0032] The instruments and reagents used are as described above.
[0033] Using non-competitive mode, add a series of amnestic shellfish toxin standards and HRP-labeled amnestic shellfish toxin antibody with different concentrations into the micro-enrichment tube, incubate in a water bath at 37°C for 30min, take it out and dilute to 200μL with buffer solution , pressure injection (9cm, 20s), run electrophoresis and record. In non-competitive mode, HRP-labeled amnestic shellfish toxin antibody (Ab*) was in excess, and the sample solution after incubation contained both HRP-labeled amnestic shellfish toxin antibody-antigen conjugate (Ag-Ab*) and untreated The reacted amnestic shellfish toxin antibody (Ab*), the amnestic shellfish toxin-enzyme-labeled antibody complex in the mixture and the remaining enzyme-labeled amnestic shellfish...
Embodiment 2
[0036] Example 2 Detection of Amnestic Shellfish Toxin in Shellfish Samples Using Capillary Electrophoresis Electrochemical Enzyme-Linked Immunoassay
[0037] Remove the shells from shellfish (scallops or oysters), soak the shellfish with distilled water for 3 times, homogenize for 10 minutes, sonicate in an ice-water bath for 10 minutes, adjust the pH to 3.4-4.0 with 6mol / L hydrochloric acid, centrifuge for 5 minutes, and pour off the supernatant. Add methanol and stir evenly, ultrasonically extract for 5 minutes, centrifuge for 5 minutes, collect the upper layer extract, evaporate the extract under reduced pressure in a water bath at 35-50°C until the methanol is volatilized, and dissolve the eluate with sodium lauryl sulfate micellar solution;
[0038] Add excess enzyme-labeled amnesic shellfish toxin antibody to the above-mentioned treated shellfish sample solution, incubate and react at 37°C for 30 minutes, amnestic shellfish toxin in the shellfish sample reacts with the e...
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