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Chromogenic medium for detecting salmonella

A chromogenic culture medium, Salmonella technology, applied in the directions of microorganism-based methods, microbial determination/inspection, microorganisms, etc., can solve the problems of inability to display characteristic color, inability to detect Salmonella typhi and Salmonella paratyphi, etc., Achieve the effect of short detection cycle, good specificity, and easy industrial production

Inactive Publication Date: 2012-12-19
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the chromogenic medium Rambach agar produced by Merck Company in Germany and some domestic brands of medium, the chromogenic medium of this principle cannot detect Salmonella typhi and Salmonella paratyphi in Salmonella, these two bacteria are here Can not show characteristic color on chromogenic medium

Method used

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  • Chromogenic medium for detecting salmonella
  • Chromogenic medium for detecting salmonella

Examples

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Effect test

Embodiment 1

[0039] A chromogenic medium for detecting Salmonella, each 1000mL medium contains 15g of agar, 12g of peptone, 5g of beef extract powder, 5g of sodium chloride, 5g of Tween-20, 5-bromo-6-chloro-3-ind Indole-octyl ester 0.35g, 5-bromo-4-chloro-3-indole-β-D-galactoside 0.4g, IPTG 0.15g, 5-bromo-4-chloro-3-indole-N-acetyl -β-D-glucosamine 0.4g, pig bile salt 6g, novobiocin 12mg.

Embodiment 2

[0041] A chromogenic medium for detecting Salmonella, each 1000mL medium contains agar 11g, peptone 15g, beef extract powder 3g, sodium chloride 7g, Tween-20 2g, 5-bromo-6-chloro-3-ind Indole-octyl ester 0.1g, 5-bromo-4-chloro-3-indole-β-D-galactoside 0.4g, IPTG 0.05g, 5-bromo-4-chloro-3-indole-N-acetyl -β-D-glucosamine 0.3g, pig bile salt 2g, novobiocin 15mg.

Embodiment 3

[0043] A chromogenic medium for detecting Salmonella, each 1000mL medium contains agar 18g, peptone 10g, beef extract powder 7g, sodium chloride 6g, Tween-20 7g, 5-bromo-6-chloro-3-ind Indole-octyl ester 0.4g, 5-bromo-4-chloro-3-indole-β-D-galactoside 0.3g, IPTG 0.3g, 5-bromo-4-chloro-3-indole-N-acetyl -β-D-glucosamine 0.5g, pig bile salt 8g, novobiocin 10mg.

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Abstract

The invention discloses a chromogenic medium for detecting salmonella, which belongs to the fields of food safety and clinical microbiological detection. The culture medium contains agar, peptone, beef extract powder, sodium chloride, a surfactant, cholate, an enzyme inducer, an octoate enzyme chromogenic substrate, a beta-galactosidase chromogenic substrate, a hexosaminidase chromogenic substrate and robiocina. The chromogenic medium disclosed by the invention is used for detecting salmonella, has high detection sensitivity and high specificity, and can be used for initially identifying strains directly according to the color of a colony; the chromogenic medium has high operability, and suitable for treating large-reflux samples, and can be used for comprehensively, systematically and accurately detecting and initially identifying salmonella in food production, clinical disease diagnosis and the environment; and a new way is provided for rapid detection of microorganisms.

Description

technical field [0001] The invention relates to a medium for detecting microorganisms, in particular to a chromogenic medium for detecting Salmonella. Background technique [0002] salmonella( Salmonella ) is an important pathogenic genus of Enterobacteriaceae. According to statistics, among the bacterial food poisoning in various countries in the world, food poisoning caused by Salmonella often ranks first. and international trade, and seriously affect social stability. According to the statistics released by the US CDC on February 9, 2009, 600 people in 44 states of the United States were infected with Salmonella by eating two kinds of peanut butter. In my country, Salmonella contamination is also the most important food safety problem. According to the survey, Salmonella accounts for 80% of the bacterial food poisoning incidents that occur in my country every year. [0003] The traditional detection method of Salmonella uses conventional biochemical principles. Since ...

Claims

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Application Information

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IPC IPC(8): C12Q1/44C12Q1/34C12Q1/04C12R1/42
Inventor 卢勉飞蔡芷荷邱国建吴清平姚华卓陈亮亮
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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