Anti-human thyroglobulin monoclonal antibody and application thereof

A monoclonal antibody and immunoglobulin technology, applied in anti-animal/human immunoglobulin, applications, antibodies, etc., can solve the problem of ineffective activation of effector systems

Inactive Publication Date: 2010-06-30
SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mouse-derived monoclonal antibodies have many limitations when applied to human treatment: first, they cannot effectively activate the effector systems related to complement and Fc receptors in the human bod

Method used

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  • Anti-human thyroglobulin monoclonal antibody and application thereof
  • Anti-human thyroglobulin monoclonal antibody and application thereof
  • Anti-human thyroglobulin monoclonal antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Preparation and Purification of Murine Monoclonal Antibody mTg-60

[0069] mouse immunization

[0070] Take Tg protein (1mg / mL) and fully emulsify with an equal volume of Freund's complete adjuvant, immunize 8-week-old female BALB / c mice by subcutaneous multi-point injection, and boost immunization with emulsified antigen in Freund's incomplete adjuvant 2 weeks apart The second time, 200 μg of purified protein antigen was injected intraperitoneally for booster immunization 3 days before cell fusion.

[0071] Cell fusion and screening of hybridoma cells

[0072] Splenocytes from immunized mice were fused with SP2 / O cells. After fusion, use HAT selection culture to culture in a 37°C, 50mL / L CO2 incubator. After fusion, half of the HAT selection medium was replaced on the 3rd and 6th day, and the HT selection medium was replaced on the 9th day. When the fused cells cover 1 / 4 to 1 / 2 of the wells, use Tg protein as the detection antigen, coat the 96-well plate with 10ug / m...

Embodiment 2

[0078] Functional identification of murine monoclonal antibody mTg-60

[0079] Functional detection was performed by solid-phase radioimmunoassay. The detection method has obtained the Shanghai Science and Technology Achievement Certificate (registration number: 931000948).

Embodiment 3

[0081] Preparation and purification of humanized monoclonal antibody hTg-37

[0082] (1) Preparation of humanized monoclonal antibody hTg-37

[0083] 1. Use PCR technology to amplify the heavy chain and light chain genes of the mouse monoclonal antibody MTg-60 hybridoma cell line, see figure 1 ;

[0084] 2. After gene sequencing, determine the sequences of heavy chain and light chain genes (SEQ ID NO: 13 and 14), design humanized primers (heavy chain SEQ ID NO: 9 and 10) and (light chain SEQ ID NO: 11 and 12);

[0085] 3. Amplify the humanized monoclonal antibody hTg-37 heavy chain and light chain genes, see figure 2 ;

[0086] 4. Double digest the recovered humanized VH and VL gene PCR products BamH I and HindIII and clone them into the pCMV_CH and pVL_CL vectors that have been digested with the same enzymes, and transform Escherichia coli DH5α with the ligated products, and culture them upside down at 37°C overnight. Pick the clone, extract the plasmid, and identify i...

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Abstract

The invention provides an anti-human thyroglobulin monoclonal antibody. The monoclonal antibody has the characteristics of high specificity and high humanization degree after being combined with the human thyrogliobulin antigen. The invention also provides an anti-human thyroglobulin monoclonal antibody immunoglobulin, a fragment and an immunoconjugate thereof as well as a pharmaceutical composition including the immunoglobulin, the fragment or the immunoconjugate. The invention also provides a detection kit for detecting the human thyrogliobulin.

Description

technical field [0001] The invention relates to the field of monoclonal antibodies, in particular to specific monoclonal antibodies against human thyroglobulin and applications thereof. Background technique [0002] Thyroid cancer is a common tumor of the endocrine system, and its incidence is higher in women than in men. At present, iodine radiotherapy is the main method for differentiated thyroid cancer, but for those who cannot absorb 131 There is no effective method for the qualitative, localized imaging and treatment of metastatic foci of poorly differentiated and undifferentiated thyroid cancer. [0003] With the development of hybridoma technology, tumor radioimmunodiagnosis and guided therapy, as a systematic and specific targeted tumor diagnosis and treatment, are being paid attention to by people. In recent years, with the preparation of high-affinity monoclonal antibody (McAb), the improvement of radionuclide labeling technology, and the improvement of tumor mic...

Claims

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Application Information

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IPC IPC(8): C07K16/18C12N15/13G01N33/577A61K39/395A61K49/00A61P35/00
Inventor 康向东
Owner SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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