Kit for measuring content of thyroglobulin and test method thereof

A thyroglobulin and kit technology, applied in the field of biochemistry, can solve problems such as timely diagnosis of unwell emergency and clinical patients, cumbersome steps of radioimmunoassay, expensive reagents, etc., and achieves improved precision, shortened reaction time, and improved detection range. enlarged effect

Inactive Publication Date: 2018-06-29
JIANGSU ZECEN BIOTECH CO LTD
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination
Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.
Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for measuring content of thyroglobulin and test method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0045] The operation procedure of adopting the test kit test sample of the present invention is as follows:

[0046] 1. Sample collection

[0047] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0048] 2. Preparation before experiment

[0049] ① Take a bottle of lotion a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit for measuring the content of thyroglobulin (Tg) in human serum by a magnetism particulate chemiluminescence method. The kit comprises a calibration product, a quality control product, an anti-reagent, a magnetic particulate reagent and a luminescent substrate, wherein anti-reagent fluorescein isothiocyanate (FITC) labeled thyroglobulin is coated with antibody and alkaline phosphatase-labeled thyroglobulin labelled antibody; the magnetic particulate reagent is a magnetic particulate and sheep anti-FITC connection. A chemiluminescence technology is combined with immune magnetic particulates, so that a reaction system close to a homogeneous phase is provided; moreover, a one-step reaction mode is adopted, so that the detection sensitivity and precision are greatly enhanced, the detection range is enlarged, the reaction time is greatly shortened, and the time from the start of sample adding to a detection result is less than 35min, being remarkably faster thanthe same kind of kit; moreover, a plurality of samples can be measured at the same time on a fully-automatic chemiluminescence instrument, high-throughput and rapid determination of the thyroglobulinis realized, high accuracy and high specificity are achieved, and the precision and detection efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of thyroglobulin (Tg) in a human body by using a magnetic particle chemiluminescence method. Background technique [0002] Thyroglobulin (Tg) is a 660ku glycoprotein secreted by the thyroid follicular epithelium. Each Tg contains about 2 thyroxine (T4) and 0.5 triiodothyronine (T3) molecules, stored in the filter In the bubble cavity. Lysosomes hydrolyze T4 and T3 on the surface of Tg and release them into the blood, while a small amount of Tg is also released into the blood, and part of Tg is secreted into the blood through the thyroid lymphatic vessels. Circulating Tg is cleared by macrophages in the liver. This human protein consists of 2767 amino acid residues. It is the storage form of iodine in the thyroid gland in the body. It can be hydrolyzed to produce thyroxine and 3,5,3′-triiodothyronine. [0003] Changes in serum TG are more co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/543G01N21/76
CPCG01N33/532G01N21/76G01N33/54326
Inventor 薛玲刘振世杨旻
Owner JIANGSU ZECEN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap