89 results about "Gracilaria lemaneiformis" patented technology

The invention discloses a gracilaria lemaneiformis agar oligosaccharide and the preparation method and application thereof and relates to an oligosaccharide. The invention provides a gracilaria lemaneiformis agar oligosaccharide, the preparation method of gracilaria lemaneiformis agar oligosaccharide and the application of the gracilaria lemaneiformis agar oligosaccharide in preparation of antioxidant and uvioresistant health care products and cosmetics. Pacific heating color bacillus H2 and gracilaria lemaneiformis are co-cultivated and the gracilaria lemaneiformis is directly degraded by the enzyme system which is generated by bacterial strain to obtain the gracilaria lemaneiformis agar oligosaccharide of which the degree of polymerization is 4-8. The gracilaria lemaneiformis agar oligosaccharide has the physiological activities of antioxidation, absorbing ultraviolet rays and the like. In preparation, the bacterial strain is activated by an activation medium plate and inoculated in a seed medium; fermentation broth is obtained by shake cultivation and inoculated in a saccharide generating medium; after shaking fermental cultivation and centrifugalization, supernate is collected; the supernate is successively filtered by a membrane filtration devices with a 0.45mu m filter membrane, a 0.22mu m filter membrane, a 10000-50000D ultrafiltration membrane and a 300-600D ultrafiltration membrane, and the percolate is the gracilaria lemaneiformis agar oligosaccharide.

The present invention discloses a water and fertilizer retention organic fertilizer made of the following raw materials by weight: 20-30 parts of sorghum straw charcoal powder, 6-8 parts of river silt, 2-3 parts of potassium mineral powder, 1-2 parts of calcium hydrogen phosphate, 4-5 parts of EDTA chelated zinc, 4-6 parts of died sweet potato, 5-6 parts of fulvic acid, 4-5 parts of durian shell, 3-4 parts of sunflower oil slag, 4-5 parts of potassium sulfate, 6-8 parts of ammonium nitrate, 35-45 parts of rape straw, 6-8 parts of gracilaria lemaneiformis, 8-10 parts of safflower seed oil residue, 20-30 parts of a soil conditioner, and the proper amount of water; the raw materials adopted by the fertilizer are wide in sources, are mainly non-pollution production waste, and effectively reduce the production cost, by a compounded coated granular structure, the fertilizer release time can be prolonged, soil water and fertilizer retention capacity can be improved, and the soil absorption, buffer and resistance properties can be improved.

The invention discloses medlar-mulberry healthcare rice wine and a preparation method thereof. The medlar-mulberry healthcare rice wine is composed of the following raw materials in parts by weight: 120-125 parts of glutinous rice, 18-25 parts of wheat germ, 20-25 parts of black rice flour, 4-8 parts of medlar, 5-10 parts of mulberry, 3-6 parts of pumpkin flower, 3-5 parts of gracilaria lemaneiformis, 2-4 parts of houttuynia cordata, 3-4 parts of tendrilleaf solomonseal rhizome, 1-2 parts of eleocharis yokoscensis, 4-5 parts of radix pseudostellariae, 2-4 parts of cordyceps sinensis, 3-4 parts of tuber fleeceflower stem, 1-2 parts of butterflybush flower, 3-4 parts of halodendron, 2-4 parts of dragon's tongue leaf and 5-6 parts of hollyhock. The rice wine disclosed by the invention is fresh in taste, mellow in flavor and sweet and delicious; the mulberry and the medlar have multiple healthcare functions, so that the medlar-mulberry healthcare rice wine has the functions of preventing and treating cancer, lowering cholesterol, preventing angiosclerosis, nourishing and whitening, engendering liquid and moistening dryness, nourishing liver and improving eyesight, enriching blood and soothing the nerves, and preventing dizziness, and the like.

The invention discloses a production method of lotus root vermicelli. The production method comprises the following steps of: weighing the following starch raw materials in parts by weight: 500-550 parts of cassava starch, 50-65 parts of glutinous rice flour and 60-70 parts of cassava starch; and weighing the following auxiliary raw materials in parts by weight: 15-18 parts of Chinese wolfberry fruit, 10-15 parts of gracilaria lemaneiformis, 10-15 parts of pitaya flower, 3-5 parts of hawk tea, 3-5 parts of dandelion, 1-2 parts of fresh rhizoma phragmitis, 3-5 parts of coprinus comatus, 10-12 parts of calabazilla, 2-5 parts of radix angelicae, 20-30 parts of water fennel, 3-5 parts of cushaw flowers, 3-5 parts of plantain herb, 1-2 parts of sea-buckthorn and 3-5 parts of potentilla chinensis. The multiple traditional Chinese medicines, edible wild herbs and the like with homology of medicine and food are added to the raw materials of the vermicelli, so that the nutrition and health care values of the vermicelli increase, and after being eaten for a long time, the lotus root vermicelli has a health care function and can regulate body functions; and then undesirable constituents such as an aluminum compound are not added to the vermicelli, and meanwhile, after the vermicelli is cooked for a long time, the soup pasting can not be caused. The lotus root vermicelli is good in elasticity, flexibility and mouth feel.

The invention relates to a method for efficiently purifying heavy metals in bodies of cultured oysters. The method comprises the steps of utilizing fresh macroalgae such as gracilaria lemaneiformis, gracilaria and ulva to be combined with chitosan and the oysters for carrying out mixed cultivation for 7-14 days, wherein the ratio of the algae to seawater is equal to (1-5)kg to 1m<3>, the ratio of the oysters to the seawater is equal to (10-20)kg to 1m<3>, and the concentration of the chitosan is 1-10mg / L; feeding the oysters with a right amount of platymonas subcordiformis, periodically replacing the seawater and supplementing the chitosan, carrying out continuous air inflation, and the like; the method can efficiently purify heavy metal ions in the bodies of the oysters; the heavy metal content of the purified oysters is significantly lower than the safety limit of first class or second class GB18421-2001 of the standard of China; the Cu<2+> purification efficiency and the Pb<2+> purification efficiency of the method are respectively 87.63-93.73% and 78.21-96.11%. The method is high in efficiency, short in period, high in practicality, environment-friendly and safe; a new method is provided for the purification of the heavy metal ions in the bodies of the oysters, and a new idea is provided for the purification of heavy metal ions in the bodies of marine shells.

The invention discloses a production method of mungbean vermicelli. The production method comprises the following steps of: weighing the following starch raw materials in parts by weight: 500-550 parts of cassava starch, 50-65 parts of glutinous rice flour and 60-70 parts of mungbean starch; and weighing the following auxiliary raw materials in parts by weight: 10-15 parts of gracilaria lemaneiformis, 10-15 parts of pitaya flower, 3-5 parts of hawk tea, 20-30 parts of mushrooms, 3-5 parts of honeysuckle flowers, 3-5 parts of sugarcane top, 3-5 parts of dandelion, 1-2 parts of hawthorn, 2-3 parts of ginger, 3-5 parts of broadleaf holly leaf, 3-5 parts of boxthorn leaves, 3-5 parts of hibiscus sabdariffa, 3-5 parts of sweet-scented osmanthus and 3-5 parts of pericarpium citri reticulatae. The multiple traditional Chinese medicines, edible wild herbs and the like with homology of medicine and food are added to the raw materials of the vermicelli, so that the nutrition and health care values of the vermicelli increase, and after being eaten for a long time, the lotus root vermicelli has a health care function and can regulate body functions; and then undesirable constituents such as an aluminum compound are not added to the vermicelli, and meanwhile, after the vermicelli is cooked for a long time, the soup pasting can not be caused. The mungbean vermicelli is good in elasticity, flexibility and mouth feel.

The invention discloses a cereal-vegetable-fruit beverage and a preparation method thereof. The cereal-vegetable-fruit beverage is prepared from the following components in parts by weight: 30-40 parts of apple, 20-30 parts of hawthorn, 200-300 parts of purified water, 40-50 parts of oat, 30-40 parts of soybean, 30-40 parts of vegetable, 10-15 parts of yam, 10-15 parts of ginkgo leaf, 5-10 parts of walnut, 3-5 parts of brown sugar, 3-5 parts of protein compound enzyme, 3-5 parts of lactic acid bacteria flora and 0.5-1 part of bacillus natto, wherein the vegetable is one or more of red spinach, spinach, gracilaria lemaneiformis, asparagus officinalis, parsley, carrot, nori and kelp. The cereal-vegetable-fruit beverage disclosed by the invention is rich in nutrition, has the effects of enhancing the immunity of the human body, reducing blood pressure and blood fat, contributing to regulation of blood vessel tensile force and the like, and can be used for protecting the health of heart and cerebral vessels of the human body.

The invention discloses a gracilaria lemaneiformis antihypertensive peptide and application thereof to preparing antihypertensive drugs and healthcare products. Gracilaria lemaneiformis antihypertensive peptide extract is obtained through enzymolysis and then further separated and purified to obtain the gracilaria lemaneiformis antihypertensive peptide with a specific sequence; the amino acid sequence of the gracilaria lemaneiformis antihypertensive peptide is Phe Gln-Ile-Asn-[Met(O)]-Cys-Ile-Leu-Arg(FQIN[M(O)]CILR), and the molecular weight of the gracilaria lemaneiformis antihypertensive peptide acquired through mass spectrum identification is 1153.43Da. The selected gracilaria lemaneiformis antihypertensive peptide is significant in activity on angiotensin converting enzyme inhibition and can in vitro express temperature, pH, gestro-intestinal digestion and angiotensin converting enzyme (ACE) degradation stability. enzyme kinetics survey shows that the gracilaria lemaneiformis antihypertensive peptide is an ACE non-competitive inhibitor and can achieve good antihypertensive effects in vitro and in vivo of spontaneously hypertensive rats (SHRs), thereby being applicable to hypertensive treatment-related healthcare products and drugs.

The invention discloses a gracilaria lemaneiformis agar oligosaccharide and the preparation method and application thereof and relates to an oligosaccharide. The invention provides a gracilaria lemaneiformis agar oligosaccharide, the preparation method of gracilaria lemaneiformis agar oligosaccharide and the application of the gracilaria lemaneiformis agar oligosaccharide in preparation of antioxidant and uvioresistant health care products and cosmetics. Pacific heating color bacillus H2 and gracilaria lemaneiformis are co-cultivated and the gracilaria lemaneiformis is directly degraded by the enzyme system which is generated by bacterial strain to obtain the gracilaria lemaneiformis agar oligosaccharide of which the degree of polymerization is 4-8. The gracilaria lemaneiformis agar oligosaccharide has the physiological activities of antioxidation, absorbing ultraviolet rays and the like. In preparation, the bacterial strain is activated by an activation medium plate and inoculated in a seed medium; fermentation broth is obtained by shake cultivation and inoculated in a saccharide generating medium; after shaking fermental cultivation and centrifugalization, supernate is collected; the supernate is successively filtered by a membrane filtration devices with a 0.45mu m filter membrane, a 0.22mu m filter membrane, a 10000-50000D ultrafiltration membrane and a 300-600D ultrafiltration membrane, and the percolate is the gracilaria lemaneiformis agar oligosaccharide.

The invention provides agarase and a preparation method thereof and a method for cloning and expression of agarase in escherichia coli and yeast cells. The nucleotide sequence of agarase is as shown in SEQ NO.1, and the amino acid sequence of agarase is as shown in SEQ NO.2. Vectors of nucleotide molecules are escherichia coli plasmids or yeast plasmids. Cells of the nucleotide molecules are obtained by transforming the vector. Cells of the nucleotide molecules of agarase are escherichia coli containing the nucleotide molecules or transformed by the vectors or Pichia pastoris containing nucleic acid molecules or transformed by the vectors. A recombination production strain which can be expressed efficiently and secrete agarase is prepared, the production industrialization of agarase is achieved, and the agarase has good capability of hydrolysis of agar and gracilaria lemaneiformis.

The invention discloses a gracilaria lemaneiformis polysaccharose-containing whitening and anti-aging mixed gel facial mask and a preparation method. The gracilaria lemaneiformis polysaccharose-containing whitening and anti-aging mixed gel facial mask is prepared from 2 to 8 weight parts of gracilaria lemaneiformis polysaccharose-containing whitening and anti-aging mixed gel facial mask powder and25 to 80 weight parts of gel facial mask water aqua. The preparation method of the whitening and anti-aging mixed gel facial mask powder comprises the following steps: fully and uniformly mixing 0.1to 2 parts of sodium alginate, 0.001 to 0.5 part of glucose, 0.01 to 0.8 part of guar gum and 0.001 to 0.5 part of calcium sulfate according to proportions, adding 0.2 to 2 parts of gracilaria lemaneiformis polysaccharose and 0.002 to 1 part of tea polyphenol, and uniformly mixing the materials. The gel facial mask water aqua is prepared by fully dissolving 0.001 to 0.2 percent of sodium pyrophosphate in water. The powder and the water aqua of the facial mask disclosed by the invention are independently packaged; no preservatives are added, so that an allergy risk caused by the preservative iseliminated, and the micro-ecological balance of the skin is maintained; furthermore, the raw materials are safe, nontoxic and environmentally friendly. The gracilaria lemaneiformis polysaccharose isused as a main active component; the tea polyphenol is further added, so that the facial mask has good effects of whitening the skin, resisting aging and the like, and the application range of the gracilaria lemaneiformis polysaccharose is expanded.

The invention discloses a rotation cropping method of high-mountain greenhouse tomatoes. The method is characterized in that the seedlings of the high-mountain greenhouse tomatoes are cultured in the middle ten days and the last ten days of April and planted from the late ten days of May to the first ten days of June, and harvesting is completed in the late ten days of November; idle greenhouses are used to plant low-temperature-resistant vegetables from the finishing of the harvesting to the tomato planting of the next year, the altitude of a high mountain is 1000-1600m, and the low-temperature-resistant vegetables are gracilaria lemaneiformis, Morchella or Chinese cabbage and the like. The method has the advantages that other crops are planted after the harvesting of the high-mountain greenhouse tomatoes, a continuous cropping is changed into a rotation cropping mode, soil utilization rate can be increased, and economic benefits are increased greatly; soil in the greenhouses can be improved favorably, and continuous cropping diseases can be inhibited.

The invention discloses an indoor rapid multiplication cultivation system for seaweed Gracilaria lemaneiformis, comprising a multi-stair support; a plurality of cultivation boxes are arranged on the multi-stair support side by side from bottom to top, a water inlet of each top cultivation box is connected with a heat changer through a water delivery pipe, an outflow pipe arranged on the upper portion of each cultivation box is extended down to the bottom of one lower cultivation box, an outflow pipe of each bottom cultivation box is connected sequentially with an inflating device, a circulating pump and the heat exchanger, and a circulating water loop is formed; support cross rods are fitted with solar lamps each above one cultivation box. The stereoscopic multi-tier multi-passage connected cultivation box structure is utilized, a flow of water is controlled to flow out from bottom to top, waves of natural sea areas are simulated, space and light are maximally utilized, and the true natural growth state of Gracilaria lemaneiformis is simulated; different cultivation interfaces can be provided, the growth environment is optimized, the system is suitable for multiplication and cultivation of Gracilaria lemaneiformis under different biomasses, and all-weather indoor large-scale multiplication cultivation of Gracilaria lemaneiformis is thus achieved.

The invention discloses a culture medium containing gracilaria-lemaneiformis algal residues and a preparing method of the culture medium. The culture medium containing the gracilaria-lemaneiformis algal residues is prepared from, 30 wt%-60 wt% of gracilaria-lemaneiformis algal residues, 30 wt%-60 wt% of grass carbon, 5 wt%-10 wt% of vermiculite and 0.01 wt%-0.05 wt% of a microbial agent, wherein the microbial agent comprises bacillus subtilis, bacillus megatherium, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and bacillus methylotrophicus, and the viable count is 1 billion / g to 20 billions / g.

The invention discloses a feed for Gushi chicken raised in bamboo groves and a preparation method thereof. The feed is prepared from the following raw materials in parts by weight: 250-280 parts of bamboo leaves, 240-260 parts of grass, 200-220 parts of corn leaves, 120-160 parts of gracilaria lemaneiformis, 40-80 parts of rod chilli leaves, 10-20 parts of loquat flower, 200-300 parts of sesame seed meal, 180-220 parts of soybean meal, 140-180 parts of oat, 160-220 parts of fly maggot, 150-180 parts of swan-mussel, 40-70 parts of chlorella powder, 30-50 parts of tomato seeds, 20-30 parts of pine nut powder, 10-20 parts of Chinese date powder, 30-40 parts of bone soup, 5-7 parts of spicy got sauce, 8-10 parts of fish bone powder, 0.2-0.5 part of chicken additive and 70-80 parts of whey powder. When the feed is used for raising Gushi chicken, Gushi chicken can grow and develop fast, and have strong bones, full plumage, strong immunity and high survival rate; the chicken meat is tight, tender and delicious in taste, the eggs are excellent in quality and rich in nutrition, and eggs and meat have high medicinal value and good health and tonifying effects.

The invention discloses gracilaria lemaneiformis flavored seafood imitated butterfish-containing crab sticks. The seafood imitated butterfish-containing crab sticks are prepared by using gracilaria lemaneiformis and butterfish as raw materials and adding cottonrose hibiscus and piper hainanense, the nutritional value of the gracilaria lemaneiformis and butterfish is sufficiently utilized and are mixed with traditional Chinese medicinal materials to synergize and achieve the effects of strengthening the spleen and tonifying the stomach. In the seafood imitated butterfish-containing crab sticks, sweet distiller's yeast is added into gracilaria lemaneiformis for fermenting, the astringent taste of the raw material is completely removed, and a unique mellow fragrance is added to the finished product. The prepared finished product has white color, is rich in elasticity and delicious in taste, has a remarkable health-care function, can be easily stored, and can be used for remarkably improving the uncomfortable feeling of people with dyspepsia. The gracilaria lemaneiformis flavored seafood imitated butterfish-containing crab sticks adopt a simple manufacture process and are easy to implement, can meet the requirement of people for health, and can be produced in volume.

The invention provides algae ferment and a preparation method thereof. The algae ferment is prepared from the following raw materials and compound microorganisms by fermentation: 60-70wt% of algae and 30-40wt% of fruits. The additive amount of the compound microorganisms is 10-16wt% of the total weight of the raw materials, wherein the compound algae are a mixture of choxmatsu gracilaria lemaneiformis algae, arthrospira, spirulina, closterium, corallina pilulifera and fFcus vesiculosis; and the fruits are a mixture of citrus, apples, grapes, strawberries, pineapples, banana, peaches, pears, apricots, muskmelons, watermelons, coconut trees and blueberries. The compound microorganisms are mixture of bacillus coagulans, lactobacillus gasseri and lactobacillus helveticus or a mixture of bacillus coagulans, lactobacillus plantarum and lactobacillus acidophilus. The preparation method comprises the following steps: performing two-stage fermentation on the compound algae, raw materials of fruits and the compound microorganisms; and then performing filtration and concentration. The ferment improves the digestibility and nutritional utilization ratio of algae.

The invention discloses a yogurt fermentation method. The method comprises the steps that (1), fresh gracilaria lemaneiformis and rosa davurica fruits are dried at first, after drying, mashing is conducted, the mixture is put into a closed container, the container is filled with air until the air pressure in the container reaches 2 standard atmospheric pressures or above, the pressure is maintained, then vacuumizing is conducted rapidly to reach 0.3 standard atmospheric pressure or below, the pressure is maintained, then gracilaria lemaneiformis and rosa davurica are taken out to be mixed with fish bone powder, the mixture is soaked in a citric acid solution, centrifugal separation is conducted, clear liquid is freeze-dried to reach the constant weight, and extracts of gracilaria lemaneiformis-rosa davurica-fish bone powder are obtained; (2), fresh cow milk and the extracts of gracilaria lemaneiformis-rosa davurica-fish bone powder are mixed and stirred to the uniform, pasteurization is conducted, after natural cooling, a leavening agent is inoculated, filling and fermenting are conducted, after fermentation is finished, refrigeration is conducted, and a finished product is obtained. Accordingly, by mixing the extracts of gracilaria lemaneiformis-rosa davurica-fish bone powder in the fermentation process of yogurt, the fermented yogurt product has better quality, the water holding rate is significantly improved, and the whey precipitation rate is significantly lowered.

The invention discloses a seaweed fermented beverage. The seaweed fermented beverage is prepared by fermentation using aroma-producing yeast. The aroma-producing yeas is named Saccharomyces cerevisiaeJJ4, and is preserved in China Center for Type Culture Collection on April 19, 2018 with the preservation number of CCTCC NO: M 2018219. The Saccharomyces cerevisiae JJ4 has good seaweed deodorizingeffect and aroma producing capability. Gracilaria lemaneiformis and other seaweeds are used as a raw material, and are processed to prepare an alga pulp, the Saccharomyces cerevisiae JJ4 is added, regulated fermentation is carried out to obtain a Gracilaria lemaneiformis fermentation solution having no algae smell and strong yeast fermentation flavor, and the yeast fermented beverage prepared by blending the fermentation solution has rich fermentation aroma, and also has the unique alga aroma of the Gracilaria lemaneiformis. The seaweed fermented beverage maintains the unique alga aroma of theGracilaria lemaneiformis, the Saccharomyces cerevisiae JJ4 fermentation is carried out to remove the alga smell of the Gracilaria lemaneiformis and makes the Gracilaria lemaneiformis have the rich yeast fermentation aroma and nutrient substances in order to obtain the new Gracilaria lemaneiformis fermented beverage product with unique flavor and refreshing mouthfeel, and the seaweed fermented beverage has good economic and social benefits.

The present invention discloses a radix puerariae blood pressure lowering health-care tea which consists of the following raw materials: 120-150 parts of radix puerariae, 40-60 parts of plumula nelambinis, 40-60 parts of cassia seeds, 6-8 parts of glutinous rice flour, 6-7 lotus leaves, 8-10 parts of winter bamboo shoots, 10-15 parts of selenium-rich matcha powder, 6-10 parts of rose petals, 1-2 parts of bamboo salt, 7-11 parts of sagittaria sagittifolia serous fluid, 5-7 parts of gracilaria lemaneiformis, 4-5 parts of distiller's yeast, 3-5 parts of herba hedyotis, 1-2 parts of pseudo-ginseng, 2-3 parts of stigmata maydis, 2-4 parts of phoenix tree leaves, 1-2 parts of salvia miltiorrhiza, and 7-9 parts of nutritional additive solution. The radix puerariae blood pressure lowering health-care tea uses radix puerariae as the main raw material which has efficacies of lowering blood pressure, lowering blood fat, lowering blood sugar, beautifying feature and protecting skin, and lowering lipid and losing weight, adds winter bamboo shoots, gracilaria lemaneiformis, etc. as accessory ingredients which are rich in vitamins and can promote metabolism, enhance immunity, and at the same time uses stigmata maydis and other various Chinese herbal medicines which have efficacies of lowering blood pressure and causing diuresis, and strengthening spleen and stomach.

The invention discloses an alga oligosaccharide fish feed additive and relates to an alga oligosaccharide. Aiming at solving the problems of the prior art, the invention provides the alga oligosaccharide fish feed additive. The alga oligosaccharide fish feed additive improves a feed utilization rate, promotes growth, improves disease resistance, reduces a cost and improves fish quality. The alga oligosaccharide of the alga oligosaccharide fish feed additive is gracilaria lemaneiformis agar oligosaccharide and is a mixture which comprises tetrasccharide to octasaccharide and is obtained through co-culture of a Flammeovirga Pacifica H2 strain and gracilaria lemaneiformis so that gracilaria lemaneiformis is directly degraded through an enzyme produced through microorganism. The Flammeovirga Pacifica H2 has a preservation number of CCTCC NO: M2012229.

The invention belongs to the technical field of food processing, and particularly relates to gracilaria lemaneiformis salad and a preparation method thereof. The gracilaria lemaneiformis salad is prepared from the following raw materials in parts by weight: 4-15 parts of gracilaria lemaneiformis, 1-3 parts of water, 0.1-0.4 part of sesame, 0.02-0.08 part of monosodium glutamate, 0.05-0.2 part of refined salt, 0.05-0.15 part of gracilaria lemaneiformis polysaccharide, 0.2-1.2 parts of chili powder, 0.1-0.6 part of pepper powder, 0.5-4 parts of milk powder, 0.5-2 parts of lemon juice, 0.2-2 parts of apple juice, 0.2-1.2 parts of cooking wine, 0.02-0.05 part of edible gum, 0.05-0.2 part of olive oil, 0.5-3 parts of emulsifier and 0.05-0.3 part of modified starch. The gracilaria lemaneiformis salad prepared by adopting the method has a good taste, and is ready-to-eat with high nutritional value. No fishy smell is the most remarkable characteristic of the gracilaria lemaneiformis salad, which can be used for solving the problem of the unacceptable fishy smell of the gracilaria lemaneiformis.

The invention discloses thermally stable mutant aromatic sulfatase and its gene and use. Through an error-prone PCR technology, random mutagenesis is introduced, a P. carrageenovora aromatic sulfatase mutant library is constructed and through screening, the mutant aromatic sulfatase with improved thermal stability is obtained. The result shows that compared with WT, H260L thermal stability is obviously improved. Based on potassium p-nitrophenyl sulfate as a substrate, the H260L has the optimal reaction temperature of 55 DEG C and pH of 8.0. The H260L is stable in the pH range of 6.0-9.0. EDTA has a strong inhibitory effect on the activity of the mutant enzyme and it is proved that a metal ion produces an important effect in the catalytic process of the mutant sulfatase. H260L has good tolerance to a detergent and has a gracilaria lemaneiformis crude polysaccharide sulfuric acid group desulfurization rate of 82%. The invention also discloses genetic engineering bacteria containing the mutant aromatic sulfatase gene. The genetic engineering bacteria realize the heterologous expression of the sulfatase and provide a good foundation for the industrial production and application of the sulfatase.

The invention discloses a Penaeus vannamei fresh-keeping agent, which is composed of the ingredients (by weight): 60-90% of Gracilaria lemaneiformis oligosaccharides, 5-20% of tea polyphenol and 1-2% of 4-hexylresorcinol. An application method of the Penaeus vannamei fresh-keeping agent comprises the following steps: 1) preparing an aqueous solution with mass concentration of 0.5-1.5% from a Penaeus vannamei fresh-keeping agent, and precooling at 3-5 DEG C to obtain a Penaeus vannamei fresh-keeping agent solution of 3-5 DEG C; and 2) immersing the Penaeus vannamei in the Penaeus vannamei fresh-keeping agent solution of 3-5 DEG C, airing after immersing, packaging with a polyethylene sealing bag, and storing. The fresh-keeping agent can inhibit blackening of Penaeus vannamei, can keep freshness of Penaeus vannamei and prolong shelf life of the product, is simple to prepare, is low-cost, and is safe to use. The invention provides a novel method for non-refrigerating fresh-keeping treatment of Penaeus vannamei.

The invention discloses a black pig feed for improving pork quality and flavor and a preparation method thereof. The black pig feed comprises the following raw materials in parts by weight: 250-280 parts of expanded soybean meal, 140-160 parts of extruded rapeseed meal, 120-140 parts of special-grade flour, 60-80 parts of corn lees, 60-80 parts of pork powder, 70-90 parts of shrimp rice flour, 50-70 parts of alfalfa, 60-80 parts of gracilaria lemaneiformis, 40-60 parts of locust tree leaves, 50-60 parts of Chinese little greens, 20-30 parts of sword bean shells, 15-20 parts of leek flower, 20-25 parts of mandragora, 30-50 parts of potatoes, 10-20 parts of Chinese bulbous onions, 10-15 parts of lotus seed powder, 15-25 parts of apple powder, 7-9 parts of pickle ends, 3-5 parts of longan meat, 1-2 parts of orchid seed powder and 2-3 parts of black pig nutrients. The feed disclosed by the invention is fine in production, safe, rich in nutrition and crisp in mouth feeling, can improve the composition of carcass and the pork quality and flavor, increase the nutritional values of pork, improve the pork quality, reduce the content of fat in the carcass, improve the lean meat percentage and promote the freshness of and the easiness in eating of the pork after being eaten by a black pig and is good in mouth feeling.

The invention discloses a gracilaria lemaneiformis extract product, a preparation method and an application thereof. The method comprises the following steps: 1) preparing novel ionic liquid 1-butyl-3-dimethylimidazolium bromides; 2) performing supersonic wave extraction on the gracilaria lemaneiformis extract product; 3) determining the alkaloid of the gracilaria lemaneiformis extract product; and 4) determining the antioxidation activity of the gracilaria lemaneiformis extract product: employing the novel ionic liquid to substitute a traditional organic solvent, the gracilaria lemaneiformis extract product having high purity, less impurity and strong antioxidation activity can be extracted by combining with the supersonic wave technology, The preparation method has the advantages of simple process, short extraction time, low temperature, low energy consumption, and high active ingredient content; recovery rate of total alkaloid in gracilaria lemaneiformis is 95.2%, the clearance of the gracilaria lemaneiformis extract product on DPPH free radical, hydroxy free radical (.OH), super-oxygen ion (O2-)free radical can reach more than 90%; the gracilaria lemaneiformis extract product can be used in the fields of novel sea innovation drugs and alga folk drugs for removing the active lead compounds, and can promote the research and exploitation of sea innovation medicine in our country.

The invention discloses liver clearing and eyesight improving chrysanthemum tea and a preparation method thereof. The liver clearing and eyesight improving chrysanthemum tea is prepared from, by weight, 15-20 parts of wild chrysanthemum flowers, 6-10 parts of pumpkin flowers, 8-10 parts of green bean flour, 2-4 parts of radix bupleuri, 4-6 parts of lotus stamens, 2-4 parts of rhizoma imperatae, 6-8 parts of radix tinosporae, 4-6 parts of radix platycodi, 2-3 parts of herba taraxaci, 2-3 parts of basella rubra leaves, 1-3 parts of snow pear juice, 2-4 parts of baby corn, 4-6 parts of gracilaria lemaneiformis, 2-3 parts of toad cortex, 6-8 parts of nutrition adding powder and an appropriate amount of white granulated sugar. The chrysanthemum tea is prepared from affinal drug and diet raw materials and has the advantages of being faint scent and sweet in mouthfeel, rich in nutrition and free of any additives, being prepared through scientific preparation, achieving the effects of clearing heat and removing toxicity, clearing the liver to improve the eyesight, whitening and beautifying the skin and smoothing the intestines to relieve constipation and being beneficial for human body health if the tea is drunk for a long term and wide in suitable crowd.

The invention discloses a cultivation method of gracilaria lemaneiformis. The method comprises the following steps of S1, breeding, putting dried and preserved gracilaria lemaneiformis seeds into a breeding basin, injecting purified water with the temperature of 25-35 DEG C into the breeding basin, fully mixing the purified water with the gracilaria lemaneiformis seeds through a stirring rod, andsoaking for 4-7 days, replacing the purified water in a cultivation basin every 12-18 h; after soaking, taking out the gracilaria lemaneiformis seeds, and draining the gracilaria lemaneiformis seeds through a strainer; S2, sand culture: taking river sand, filtering out large-diameter gravel in the river sand; mixing the gracilaria lemaneiformis seeds subjected to water draining with river sand, flatly paving a mixture of the gracilaria lemaneiformis seeds and the river sand in the cultivation basin, and injecting purified water into the cultivation basin, so that the water content of the mixture of the gracilaria lemaneiformis seeds and the river sand is 70-80%. The method is reasonable in design, the germination rate of seeds can be increased, the influence of plant diseases, insect pestsand fertility on gracilaria lemaneiformis young plants can be reduced, and then the yield of gracilaria lemaneiformis can be guaranteed.

The invention discloses an algae-containing healthy tea liquor and a production method thereof. The algae-containing healthy tea liquor comprises the following active ingredients in parts by weight: 12-15 parts of spirulina, 8-10 parts of undaria pinnatifida, 4-6 parts of gracilaria lemaneiformis, 0.4-0.6 part of red algae extract, 1-3 parts of cortex acanthopanacis, 3-5 parts of trigonella foenum-graecum, 2-4 parts of fructus psoraleae, 1-2 parts of fructus rosae laevigatae, 8-10 parts of hemisteptia lyrata, 3-5 parts of aralia elata(miq)seem, 2-4 parts of toona sinensis (a.juss.) roem. leaves, 20-30 parts of wheat germ meal, 10-12 parts of tapioca flour, 4-6 parts of foxtail millet seed meal, 2-4 parts of carp eyes, 1-2 parts of japanese farfugium herb, 1-2 parts of angelica morii hayara, 8-10 parts of condiment, and 5-6 parts of complex nutrient. The algae-containing healthy tea liquor is sweet, acidic and mellow in mouthfeel, has no algae smell, and is unique in flavor; in the formula, the hemisteptia lyrata and aralia elata(miq)seem can be used for refreshing the spirit and restoring consciousness, stimulating the stomach and digesting food, and dispelling the effects of alcohol and detoxifying, the trigonella foenum-graecum, fructus psoraleae and fructus rosae laevigatae can be used for promoting blood circulation to remove blood stasis, tonifying the liver and strengthening the spleen, nourishing yin and tonifying yang and strengthening the bones and muscles, and the immunity of the organism can be enhanced together with the synergism with health functions of carp eyes, and the algae-containing healthy tea liquor is suitable for long-time drinking.