Ternary compound inoculant for fermentation bed for pigs
A compound bacterial agent and ternary compound technology, which is applied in the field of microbial engineering, can solve the problems of waste of resources and labor, centralized discharge of pig manure, pollution of breeding environment and surrounding environment, etc., so as to prolong the service life and reduce the effect of material transformation.
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Embodiment 1
[0028] Example 1 Preparation of ternary compound bacterial agent
[0029] The pig fermentation bed ternary composite bacterial agent of the present invention is composed of composite bacterial agent I, composite bacterial agent II and composite bacterial agent III.
[0030] The compound inoculum I is a starter inoculum, which is composed of the following components in terms of weight percentage: 30% Trichoderma Corning, 30% Bacillus licheniformis, and Bacillus amyloliquefaciens 40%;
[0031] The compound inoculum II is a maintenance inoculum, which is composed of the following components according to weight percentage: Azotobacter sphaeroides 20%, Enterococcus faecalis 20%, Bacillus Laterosporus 15%, Bacillus amyloliquefaciens 25%, Bacillus subtilis 20%;
[0032] The compound inoculum III is an accumulation inoculum, which is composed of the following components according to weight percentage: Aspergillus oryzae 15%, Streptomyces 20%, Azotobacter sphaeroides 25%, Trichoderma corningii...
Embodiment 2
[0047] Example 2 Testing the effect of pig fermentation bed made by the ternary compound bacterial agent of the present invention
[0048] From July 2013 to October 2013, a pig farming experiment was carried out on the fermentation bed breeding technology of the product of the present invention. The litter is composed of sawdust and rice husk in a weight ratio of 1:1. According to the principle of the birth date and similar body weight, 60 hybrid pigs of the same breed and batch were selected and randomly divided into 2 groups with 30 pigs in each group. The test I group is the control group, a traditional cement fence; the test II group, the pig fermentation bed made of the ternary compound bacterial agent of the present invention is used for breeding.
[0049] Before entering the pig, use the compound microbial agent I (starting microbial agent), press 30 g / m 2 Add the compound bacterial agent I in the proportion of the dosage, and dissolve the compound bacterial agent I with 2L...
Embodiment 3
[0059] Example 3 Detecting the bed body effect of the ternary compound bacterial agent of the present invention in the production of fermented bed pigs
[0060] From October 2013 to January 2014, the application experiment of the product of the present invention on the bed surface substrate was carried out in a pig farm.
[0061] Test group 1: Commercially available foreign fermentation bed inoculants, containing Bacillus subtilis, amylase, and protease, with 10 viable bacteria 7 cfu / g, the additive dosage is 200g / m 3
[0062] Test Group 2: Sell domestic fermentation bed inoculants, containing lactic acid bacteria, spores, yeasts, the number of live bacteria is 10 9 cfu / g, additive dosage is 20g / m 2
[0063] Test group 3: The ternary compound inoculum of the present invention uses compound inoculum Ⅰ (starting inoculum) before entering pigs at a rate of 30 g / m 2 Add in the proportion of, 45 days after entering the pig, use the complex bacteria agent II (maintenance bacteria agent). Pr...
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