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Use of Shewanella as feed additive in abalone culture

A feed additive, Shewanella technology, applied in the application, animal feed, animal feed and other directions to achieve the effect of promoting nutrient absorption

Inactive Publication Date: 2012-10-10
DALIAN HAIBAO FISHERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there has been no application of Shewanella as a feed additive for abalone farming (mixed in the feed or sprinkled in the tank)

Method used

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  • Use of Shewanella as feed additive in abalone culture
  • Use of Shewanella as feed additive in abalone culture
  • Use of Shewanella as feed additive in abalone culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Isolation and screening of Shewanella WA64 and WA65 strains

[0018]1. Abalone source

[0019] Collected from Dalian sea area.

[0020] 2. Isolation of Shewanella WA64 and WA65 strains

[0021] Dissect and homogenize the intestinal tract of healthy abalone wrinkled under sterile conditions, spread it on 2216E solid medium after gradient dilution, and incubate at a constant temperature of 25°C for 72~96h; pick the strains with the morphology shown in Table 1 to isolate and purify, and then inoculate 2216E The slant medium was stored for later use; the components and proportions of the 2216E medium were: 0.5g peptone, 0.1g yeast extract, 0.001g ferric phosphate, 1000mL seawater, pH 7.2~7.4, sterilized at 121°C for 20min.

[0022] Table 1 Culture characteristics of Shewanella WA64 and WA65 strains

[0023]

[0024] 3. Screening of Shewanella WA64 and WA65 strains

[0025] Indicator pathogenic bacteria: 4 strains of indicator pathogenic bacteria were isolated ...

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Abstract

The invention discloses a use of Shewanella as a feed additive in the abalone culture. Shewanella (comprising WA64 and WA65 strains) has strong a capability of antagonism of abalone pathogenic bacteria and thus Shewanella can be used as a feed additive for abalone culture. The Shewanella as feed additive for abalone culture has the advantages that Shewanella promotes that juvenile abalones vie with pathogenic bacteria for adhesion sites, maintains a normal microecological balance of the intestinal canal, can colonize in the intestinal canal of an abalone, promotes nutrient absorption, improves immunity thereby improving a growth speed and disease resistance of a Haliotis discus hannai, can replace a chemical drug for disease treatment, and does not damage the ecological environment.

Description

technical field [0001] The invention relates to a new application of Shewanella, in particular to the application of Shewanella as a feed additive in abalone cultivation. Background technique [0002] Wrinkled plate abalone ( Haliotis discuss hannai ) is an important marine biological resource in my country. It is naturally distributed in the Bohai Sea, the Yellow Sea and the Sea of ​​Japan. It is known as the soft gold of the ocean and ranks first among the eight seafood treasures. It has high nutritional and medicinal values. However, the slow growth of abalone is one of the main problems faced by the abalone aquaculture industry. Although the growth rate of abalone can be accelerated through artificial feeding and bait, it still takes 3 to 4 years to reach the market standard of 80 mm; The application of compound bait will also bring problems such as rapid deterioration of water quality, low feed coefficient and serious disease occurrence. In order to further increase t...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16C12N1/20C12R1/01
Inventor 李丹刘小林常亚青姜海峰许淑芬王高学冷晓飞高洪涛邹闯于海洋宋爱玲
Owner DALIAN HAIBAO FISHERY
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