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Preparation method of Muscovy duck parvo novel vaccines

A technology of Muscovy duck parvovirus and Muscovy duck, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve problems such as uncontrollable disease and economic losses in the duck industry

Active Publication Date: 2010-11-10
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

The use of various antibiotics, Muscovy duck parvovirus vaccine and high immune yolk antibody can not control the disease, causing great economic losses to the duck industry

Method used

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  • Preparation method of Muscovy duck parvo novel vaccines
  • Preparation method of Muscovy duck parvo novel vaccines

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with specific examples. (but not a limitation of the invention).

[0011] Derived from the artificially attenuated Muscovy duck parvovirus attenuated P1 strain (MPV-P1 strain), it is artificially bred and weakened by using biotechnologies such as Muscovy duck embryos and Muscovy duck embryo fibroblasts to pass alternately. The pathogenicity of young Muscovy ducks, and has the following characteristics (1) Virus titer: MPV-P1 strain was inoculated with 12-day-old Muscovy duck embryos through the allantoic cavity, and its ELD 50 Should be ≥10 -4.5 / 0.1ml; TCID for muscovy duck embryo fibroblast monolayer cells 50 ≥10 -5.0 / 0.1ml; (2) safety: use 10 feathers of 1-day-old healthy susceptible young muscovy ducks, inject 0.2 milliliters of the poison seed stock solution into the legs of each feather, observe for 20 days, and all should survive; (3) immunogenicity: One-day-old healthy sensitive muscovy du...

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Abstract

The invention relates to a preparation method of Muscovy duck parvo novel vaccines, which comprises the following steps of: using Muscovy duck parvovirus attenuated virus P1 strain (MPV-P1 strain with a preservation number of CCTCC NO:V201013) and Muscovy duck origin goose parvovirus attenuated virus D strain ( GPV-D strain with a preservation number of CCTCC NO:V201014) as seed viruses; proliferating viruses by applying the muscovy duck embryone fibroblast spinner culture technology to obtain cell toxic liquids; mixing the cell toxic liquids according to the proper proportion and freezing a dry protective agent to freeze and dry to research safe and effective Muscovy duck parvo novel vaccines. The young Muscovy ducks are inoculated once so as to prevent and control the Muscovy duck parvovirus infection and the Muscovy duck gosling blast dieases at the same time, thereby the stress reaction of the Muscovy ducks caused by immunization for many times is solved. The invention is suitable for scale production under the GMP (Good Manufacturing Practices) condition, and saves the production cost of the vaccines.

Description

technical field [0001] The invention relates to a preparation method of a new type of Muscovy duck parvo vaccine. Background technique [0002] Muscovy duck parvovirus disease (commonly known as "three-week disease") is a new disease first discovered in our province in 1987 in 1-3 week-old muscovy ducks with diarrhea as the clinical symptom. The disease mainly affects young muscovy ducks of 7-21 days of age, with a morbidity rate of 27-62% and a case fatality rate of 22-43%. In terms of research on the pathogen, diagnosis and prevention of this disease, our institute is at the leading level in the same kind of research in the world. We isolated and identified Muscovy duck parvovirus (MPV) at home and abroad for the first time, and confirmed that MPV is the pathogen of Muscovy duck parvovirus disease. It is a new member of the genus Parvovirus in the family Parvoviridae, which enriches the content of animal virology. On this basis, five monoclonal antibodies were establishe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/23A61P31/20C12R1/93
Inventor 陈少莺程晓霞林锋强陈仕龙程由铨朱小丽林天龙胡奇林王劭
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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