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Desert oligotrophic bacterium DOB150 and application thereof to sand stabilization

An oligotrophic, CGMCCNO.11925 technology, applied in the direction of application, bacteria, and microbial-based methods, can solve problems such as the application of oligotrophic bacteria that have not been seen, and the few reports of oligotrophic microorganisms, and achieve fast growth rate and good growth , the effect of strong growth and reproduction ability

Inactive Publication Date: 2016-06-08
XINJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature search showed that, from an environmental point of view, the current oligotrophic bacteria are mainly researched on the water environment, but there are not many reports on oligotrophic microorganisms resistant to barren environments in dry desert areas, and there are no new discoveries similar to oligotrophic bacteria. A report on the application of vegetative bacteria in the fixation of quicksand in biological crusts

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  • Desert oligotrophic bacterium DOB150 and application thereof to sand stabilization
  • Desert oligotrophic bacterium DOB150 and application thereof to sand stabilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation, screening and identification of oligotrophic bacteria Bacillussp.DOB150CGMCCNO.11925

[0020] 1. Isolation and screening of strains

[0021] The desert oligotrophic bacteria used in the present invention are sampled and isolated from the Gurbantunggut Desert in Junggar Basin, Xinjiang, and the bacterial strains capable of growing on LB medium are preliminarily screened out according to the analysis of the action mechanism of the bacteria. The microorganisms in the Gurbantunggut Desert in the Junggar Basin of Xinjiang were isolated by the traditional plate culture method, the strains were screened by primary screening and secondary screening, and the strains were purified by the plate streaking method. For growth and reproduction, a batch of well-growing microbial strains were screened out, and a desert oligotrophic bacterial strain numbered DOB150 with good comprehensive performance was selected from them.

[0022] Separation steps: adopt the grad...

Embodiment 2

[0035] Example 2: Molecular level identification of oligotrophic bacteria Bacillussp.DOB150CGMCCNO.11925

[0036] 1. PCR amplified DNA sequence of oligotrophic bacteria DOB150 and its sequencing

[0037] Pick a small amount of single colony of oligotrophic bacteria DOB150 strain, put it into an EP tube filled with 25 μL of sterile water, boil it at 100°C for 8-10 minutes, and then quickly put it into the ice-water mixture for 5 minutes. Centrifuge at 10000r / min for 5min, store at 4°C, and take the supernatant when used.

[0038] Determination of 16SrDNA gene sequence and construction of phylogenetic tree: The total DNA of oligotrophic bacteria DOB150 strain was extracted according to conventional methods, and the diluted universal primer was used for PCR amplification of 16SrDNA segment with deionized water.

[0039] The 50 μl reaction system contained: 5 μl of 10×PCR buffer, 20 pmol of each primer, 1 μl of template DNA, 0.5 U of TaqTM (TaKaRa Company), and 8 μl of dNTP. PCR...

Embodiment 3

[0045] Example 3: Research and Application of Oligotrophic Bacteria Bacillussp.DOB150CGMCCNO.11925

[0046] 1. Experimental process

[0047] The oligotrophic bacterium DOB150 was repeatedly streaked and activated on LB medium, and then used for the preparation of liquid bacterial agent. After inoculation, it was cultured in a shake flask at 37°C for 60 hours.

[0048] (1) Sand fixation test

[0049] Use quicksand to pack 2 disks with a length of 30cm × width 20cm × height 5cm, put the quicksand into the tray, and spray the oligotrophic bacteria DOB150 cultivated by shaking on the shaker on the quicksand surface of one of the sand trays, and the other sand tray. An equal amount of tap water served as a control.

[0050] (2) Water retention test

[0051] Take 20 crucibles with known drying constant weight (wherein 10 are sprayed with water for comparison, and 10 are sprayed with bacterium liquid of oligotrophic bacteria DOB150) and weighed, denoted as W 1 , respectively weig...

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Abstract

The invention discloses a desert oligotrophic bacterium DOB150 and application thereof to sand stabilization. Through isolation, screening, selective breeding and acclimatization in Gurbantunggut Desert of Junggar Basin of Xinjiang, the oligotrophic bacterium Bacillus sp. DOB150 CGMCC NO.11925 is obtained as a novel bacterium. The desert oligotrophic bacterium DOB150 and application thereof to sand stabilization have the advantages that after being sprayed into the desert, a bacterium agent prepared from the isolated oligotrophic bacterium DOB150 can play a role in sand stabilization, sand grain adhesion and quicksand activation control and provides good moisture conditions for germination of some plant seeds slow in germination to enable plants incapable of germinating due to insufficient moisture of soil to grow and develop, the number of major microorganisms in the soil can be increased effectively, major nutrient content of the soil can be increased, partial soil enzyme activity can be enhanced, algal crust can be recovered obviously, and accordingly the desert oligotrophic bacterium DOB150 is widely applicable.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to the technical field of the application of a new oligotrophic bacterium in biological crust fixing quicksand. Background technique [0002] Oligotrophic bacteria are a kind of microorganisms that grow under the condition of very low nutrient concentration. They belong to special environmental microbial groups and have very low nutritional requirements for survival. Their diversity and biomass are in the composition of the entire biosphere. It has great advantages and thus plays an important role in the biogeochemical cycle. Since the 1990s, the oligotrophic mechanism of oligotrophic bacteria in natural or artificial environments, their physiological responses to starvation, and their role in ecosystems have always been one of the frontiers of microbial ecology research. It has been widely valued by microbial ecologists and environmental scientists in various countri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/205C12R2001/07
Inventor 毛培宏吕杰王军钱卫东
Owner XINJIANG UNIVERSITY
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