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A Desert Oligotrophic Bacteria dob150 and Its Application in Sand Fixation

An oligotrophic, CGMCCNO.11925 technology, applied in the direction of application, bacteria, and microbial-based methods, can solve the problems of rare oligotrophic microorganism reports and no application of oligotrophic bacteria, and achieve fast growth rate and good growth , The effect of stabilizing genetic characteristics

Inactive Publication Date: 2018-10-19
XINJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature search showed that, from an environmental point of view, the current oligotrophic bacteria are mainly researched on the water environment, but there are not many reports on oligotrophic microorganisms resistant to barren environments in dry desert areas, and there are no new discoveries similar to oligotrophic bacteria. A report on the application of vegetative bacteria in the fixation of quicksand in biological crusts

Method used

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  • A Desert Oligotrophic Bacteria dob150 and Its Application in Sand Fixation
  • A Desert Oligotrophic Bacteria dob150 and Its Application in Sand Fixation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation, screening and identification of oligotrophic bacteria Bacillus sp.DOB150CGMCC NO.11925

[0020] 1. Isolation and screening of strains

[0021] The desert oligotrophic bacteria used in the present invention are sampled and isolated from the Gurbantunggut Desert in Junggar Basin, Xinjiang, and the strains capable of growing on LB medium are preliminarily screened out according to the analysis of the action mechanism of the bacteria. The microorganisms in the Gurbantunggut Desert in the Junggar Basin of Xinjiang were isolated by the traditional plate culture method, the strains were screened by primary screening and secondary screening, and the strains were purified by plate streaking method, and the bacterial strains were compared on the LB medium. For growth and reproduction, a batch of well-grown microbial strains were screened out, and a desert oligotrophic bacterial strain numbered DOB 150 was selected with good comprehensive performance.

[002...

Embodiment 2

[0035] Example 2: Molecular identification of oligotrophic bacteria Bacillus sp.DOB150CGMCC NO.11925

[0036] 1. PCR amplified DNA sequence of oligotrophic bacteria DOB150 and its sequencing

[0037] Pick a small amount of single colony of oligotrophic bacteria DOB150 strain, put it into an EP tube filled with 25 μL of sterile water, boil it at 100°C for 8-10 minutes, and then quickly put it into the ice-water mixture for 5 minutes. Centrifuge at 10000r / min for 5min, store at 4°C, and take the supernatant when used.

[0038] 16S rDNA gene sequence determination and phylogenetic tree construction: Extract the total DNA of oligotrophic bacteria DOB150 strain according to conventional methods, and use deionized water to dilute the universal primers for PCR amplification of 16S rDNA segments.

[0039] The 50 μl reaction system contained: 5 μl of 10×PCR buffer, 20 pmol of each primer, 1 μl of template DNA, 0.5 U of TaqTM (TaKaRa Company), and 8 μl of dNTP. PCR amplification condi...

Embodiment 3

[0045] Example 3: Characteristic research and application of oligotrophic bacteria Bacillus sp.DOB150CGMCC NO.11925

[0046] 1. Experimental process

[0047] The oligotrophic bacterium DOB150 was repeatedly streaked and activated on LB medium, and then used for the preparation of liquid bacterial agent. After inoculation, it was cultured in a shake flask at 37°C for 60 hours.

[0048] (1) Sand fixation test

[0049] Use quicksand to pack 2 disks with a length of 30cm × width 20cm × height 5cm, put the quicksand into the tray, and spray the oligotrophic bacteria DOB150 cultivated by shaking on the shaker on the quicksand surface of one of the sand trays, and the other sand tray. An equal amount of tap water served as a control.

[0050] (2) Water retention test

[0051] Take 20 crucibles with known drying constant weight (wherein 10 are sprayed with water for comparison, and 10 are sprayed with bacterium liquid of oligotrophic bacteria DOB150) and weighed, denoted as W 1 , ...

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Abstract

The invention discloses a desert oligotrophic bacterium DOB150 and its application in sand fixation. An oligotrophic bacterium Bacillus sp.DOB150 CGMCC NO.11925 was obtained through isolation, screening, breeding, and domestication in the Gurbantunggut Desert in the Junggar Basin, Xinjiang. It is a new strain. By using the isolated After the bacterial agent prepared by the oligotrophic bacteria DOB150 strain is sprayed in the desert, it can not only play a role in sand fixation, but also may provide better water conditions for the germination of some plant seeds with a slow germination rate, so that some plants that cannot germinate due to insufficient soil moisture The germinated plants grow and develop; after the prepared bacterial agent is sprayed into the desert, it not only plays a role in sticking sand particles and preventing the activation of quicksand, but also effectively increases the number of main microorganisms in the soil, increases the content of main nutrients in the soil, and enhances the activity of some soil enzymes. And can obviously restore the algae crust, and has wide applicability.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to the technical field of the application of a new oligotrophic bacterium in biological crust fixing quicksand. Background technique [0002] Oligotrophic bacteria are a kind of microorganisms that grow under the condition of very low nutrient concentration. They belong to special environmental microbial groups and have very low nutritional requirements for survival. Their diversity and biomass are in the composition of the entire biosphere. It has great advantages and thus plays an important role in the biogeochemical cycle. Since the 1990s, the oligotrophic mechanism of oligotrophic bacteria in natural or artificial environments, their physiological responses to starvation, and their role in ecosystems have always been one of the frontiers of microbial ecology research. It has been widely valued by microbial ecologists and environmental scientists in various countri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/205C12R2001/07
Inventor 毛培宏吕杰王军钱卫东
Owner XINJIANG UNIVERSITY
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