Lactobacillus gasseri for preventing and treating urogenital infection and application thereof

A technology for Lactobacillus gasseri and urinary infection, applied in the field of microorganisms, can solve problems such as inconvenience during use

Active Publication Date: 2022-03-22
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, most Lactobacillus gasseri are directly administered through the vagina to treat vaginal inflammation, and it is inevitably inconvenient during use

Method used

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  • Lactobacillus gasseri for preventing and treating urogenital infection and application thereof
  • Lactobacillus gasseri for preventing and treating urogenital infection and application thereof
  • Lactobacillus gasseri for preventing and treating urogenital infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0082] Basic experiment example 1 Isolation of Lactobacillus gasseri strain

[0083] The composition of MRS medium is as follows: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; sodium acetate (CH 3 COONa·3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0g; Magnesium sulfate (MgSO4 7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjusted to pH6.2-6.6.

[0084] The separation steps are as follows:

[0085] (1) Collect 80 cases of vaginal secretion samples from healthy women without symptoms and signs of vaginitis clinically, number the samples sequentially, inoculate them in MRS liquid medium, and incubate anaerobically at 37°C for 24 hours.

[0086] (2) For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially.

[0087] (3) Amplify bacteria for bacteri...

Embodiment 1

[0096] Example 1 Lactobacillus gasseri MH-501 biochemical reaction experiment

[0097] Physiological and biochemical tests on Lactobacillus gasseri MH-501, including catalase test, gelatin liquefaction test, H 2 S test, nitrate reduction test and exercise test for identification:

[0098] 1. Catalase test

[0099] Pick out a single purified colony, place it on a new glass slide, and add a few drops of 0.3% hydrogen oxide solution. As a result, on the contrary, no bubbles are generated as a negative (-) result.

[0100] 2. Gelatin liquefaction experiment

[0101] Pick the purified single colony with an inoculation needle and inoculate it on a gelatin solid medium by puncture, the puncture depth is about 2 / 3 of the medium, culture in an incubator at 20°C for 7 days, take it out every 24h, put it in a refrigerator at 4°C for 30min, and then take it out again. It is a positive (+) result if it does not coagulate within a certain period of time, and a negative (-) result if coa...

Embodiment 2

[0111] Example 2 Carbohydrate acid production test of Lactobacillus gasseri MH-501

[0112] Lactobacillus gasseri MH-501 was biochemically identified by the Mérieux API50 CH identification system, and the fermentation reactions were positive: D-glucose, D-fructose, D-mannose, D-galactose, N-acetyl - Glucosamine, amygdalin, arbutin, escin, salicin, cellobiose, maltose, lactose, sucrose, trehalose, raffinose, starch, gentiobiose. Those that do not ferment and have a negative reaction are: glycerin, erythritol, D-arabinose, L-arabinose, D-ribose, D-xylose, L-xylose, asdol, β-methyl-D- Xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, mannitol, sorbitol, α-methyl-D-mannitol glycoside, α-methyl-D-glucoside, honey Disaccharide, inulin, melezitose, glycogen, xylitol, D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabino Sugar alcohol, L-arabinitol, gluconate, 2-keto-gluconate.

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Abstract

The invention provides a strain of lactobacillus gasseri, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.20696, and the lactobacillus gasseri is preserved in China General Microbiological Culture Collection Center on September 22, 2020. The lactobacillus gasseri developed by the lactobacillus gasseri provided by the invention has the advantages of safety, reliability, less adverse reaction, good effect and the like, has a good treatment effect on urogenital infection, particularly has a treatment effect of improving female urogenital tract infection, can also recover the micro-ecological environment of vagina fundamentally, and is beneficial to female health.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Lactobacillus gasseri used for preventing and treating urogenital infection and its application. Background technique [0002] Urogenital tract infection is one of clinical common diseases, common clinical types include bacterial vaginosis (BV), vulvovaginal candidiasis (VVC) and trichomoniasis etc., genitourinary tract infection is mostly Escherichia coli or golden Staphylococcus aureus, Gardnerella vaginalis and Candida albicans infection have a prevalence rate as high as 40%; their treatment mainly relies on antibiotics, but the cure rate is low, and the recurrence rate can reach more than 40%. [0003] At present, when antibiotics are used clinically to prevent and treat urogenital infections, although a large number of pathogenic bacteria can be effectively killed in a short period of time, a healthy micro-ecological environment cannot be restored, and t...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20C12N1/02C12N1/04A61K35/747A61K9/19A61K47/42A61P13/00A61P15/00A61P15/02A61P31/04A61P31/02A61P31/10A23L33/135A23C9/123A23L2/39A23L7/10A23L19/09A23L2/38A23G9/363
Inventor 林枫翔侯莹秦琦韩雪
Owner 哈尔滨美华生物技术股份有限公司
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