Trichoderma reesei strain capable of producing cellulase with high yield through space mutation
A technology of Trichoderma reesei and cellulase, applied in the direction of enzymes, enzymes, fungi, etc., can solve the problems of high blindness, heavy workload, and many test materials, and achieve the effect of broad industrial application prospects
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Embodiment 1
[0028] 1) Preliminary screening: The spore freeze-dried powder after space mutagenesis was gradiently diluted with sterile saline to make the spore concentration 10 3 cells / mL, take 0.05mL of the diluted sample, spread it on sterile cellulose-Congo red screening medium (121°C, sterilize for 30min), culture at 28°C for 36h, and select the transparent circle / colonies with larger diameter 1000 bacterial strains; wherein, the composition of the cellulose-Congo red screening medium is uniform: microcrystalline cellulose 10g, glucose 2g, agar 18g, Congo red 0.2g, Mandels inorganic nutrient salt 1000mL, pH value natural;
[0029]2) Re-screening: The 1,000 strains selected in step 1) were inserted into the PDA slant medium, and the spores were covered with the slant, and the spores were washed with sterile saline to prepare 10 strains. 7 Each / mL spore suspension was inserted into the enzyme production medium (50mL / 250mL liquid volume) of the Erlenmeyer flask with 10% inoculum volume (...
Embodiment 2
[0039] 1) Preliminary screening: The spore freeze-dried powder after space mutagenesis was gradiently diluted with sterile saline to make the spore concentration 10 3 cells / mL, take 0.05mL of the diluted sample, spread it on sterile cellulose-Congo red screening medium (121°C, sterilize for 30min), culture at 30°C for 42h, and pick out the transparent circle / colonies with larger diameter 1000 bacterial strains; wherein, the composition of the cellulose-Congo red screening medium is uniform: microcrystalline cellulose 10g, glucose 2g, agar 18g, Congo red 0.2g, Mandels inorganic nutrient salt 1000mL, pH value natural;
[0040] 2) Re-screening: The 1,000 strains selected in step 1) were inserted into the PDA slant medium, and the spores were covered with the slant, and the spores were washed with sterile saline to prepare 10 strains. 7 Each / mL spore suspension was inserted into the enzyme production medium (50mL / 250mL liquid volume) of the Erlenmeyer flask with 10% inoculum volum...
Embodiment 3
[0049] 1) Preliminary screening: The spore freeze-dried powder after space mutagenesis was gradiently diluted with sterile saline to make the spore concentration 10 3 cells / mL, take 0.05mL of the diluted sample, spread it on sterile cellulose-Congo red screening medium (121°C, sterilize for 30min), culture at 30°C for 48h, and select the transparent circle / colonies with larger diameter 1000 bacterial strains; wherein, the composition of the cellulose-Congo red screening medium is uniform: microcrystalline cellulose 10g, glucose 2g, agar 18g, Congo red 0.2g, Mandels inorganic nutrient salt 1000mL, pH value natural;
[0050] 2) Re-screening: The 1,000 strains selected in step 1) were inserted into the PDA slant medium, and the spores were covered with the slant, and the spores were washed with sterile saline to prepare 10 strains. 7 Each / mL spore suspension was inserted into the enzyme production medium (50mL / 250mL liquid volume) of the Erlenmeyer flask with 10% inoculum volume ...
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