45results about How to "Obvious heterosis" patented technology

The invention relates to a method for breeding a novel bee species by breeding a hybrid of east bees and west bees, comprising introducing a west bee brood comb going to come out cells into a swarm of east bees preparing for laying eggs, to make the number of east worker bees equal to that of west worker bees, and obtaning a mixed swarm; the east queen bee laying eggs on empty combs, moving out the east queen bee to make the swarm without a queen, after the first generation east worker bees have got out cells; putting in a queen cell frame 20-22 hours later, breeding a queen with four-day old east bee grubs of same swarm to get a novel east queen bee; and hybridizing said queen as mother with west male bee as father by artificial insemination or natural copulation to obtain a hybrid thereof. The method breaks the reproductive isolation between two different species, and the hybrid obtained thereby has a remarkble heterosis.

The invention discloses a method for breeding a meat and wool dual-purpose new variety of Gansu alpine merino, which comprises: crossing a South African mutton merino serving as a male parent and a Gansu alpine merino serving as a female parent, and selecting first-generation hybrid female merinos; crossing the select first-generation hybrid female merinos with South African mutton merinos, and selecting second-generation hybrid male and female merinos; crossing the selected second-generation hybrid female merinos with South African mutton merinos and selecting third-generation hybrid female merinos; mating the third-generation hybrid female merinos with the selected second-generation hybrid male merinos to breed the zero-generation merinos of the meat and wool dual-purpose new variety of Gansu alpine merino; selecting high-quality male merinos and female merinos; and performing 3 to 4 generations of crossing by a group secondary selection method and obtaining the meat and wool dual-purpose new variety of Gansu alpine merino. The merinos bred by the breeding method have the advantages of tall and big body, high lambing percentage, quick growth, high wool quality, high meat quality and the like, and are suitable for drylot feeding and pen feeding for fattening. The method is in accordance with the development trend of sheep breeding industry of China.

The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a hybrid seed production method of high uniformity yellow chicken three-line combination suitablefor chilled marketing. According to the method, a slow-feathering, yellow-feathering and green-shank chicken line is adopted as a first male parent, a fast-feathering, yellow-feathering, yellow-shank,high-yield and high-quality chicken line is adopted as a first female parent, and hybridization is conducted to obtain an F1 female parent; a local chicken line which is fast-feathering, and yellow-feathering, jute-feathering, sexual precocity and big cockscomb in appearance with the weight within + / -10% of the average weight is selected as a terminal sire, and hybridization is conducted betweenthe terminal sire and the F1 female parent to obtain commercial generation with high uniformity. According to the method, by means of regular and molecular breeding, selections of physical conformation, sexual precocity, weight uniformity and slaughter traits and comprehensive selection of meat quality traits are focused on and carried out, the bred yellow chicken commercial generation is high inuniformity and survival rate, and significant in heterosis; and the chicken has big cockscomb, green shank, fine pores and firm skin.

The invention relates to the technical field of animal breeding and particularly provides a breeding method of a high-quality synthetic white pig line. The method utilizes existing domestic and overseas pig breed resources, a specialized line is bred according to a trait differentiation and selection principle, thus commercial pigs quicker to grow, higher in lean meat percentage, good in meat quality, strong in resistance and high in slaughtering weight uniformity are obtained through hybrid breeding of the specialized line, the problem existing in 'inner binary' and 'inner ternary' breeding systems can be solved, meanwhile local pig breed resources can be also effectively protected and utilized, high-quality pig breeds are provided for industrial large-scale breeding, and the heavy demands for high-quality pork of masses of consumers are met.

The invention discloses a breeding method of a novel variety of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia includes using a male sterile line SH-B-18 of red-rooted salvia as a female parent and a fertile line A-107 of red-rooted salvia as a male parent, and preparing the female parent and the male parent into a hybrid of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia is applicable to novel high-yield, stable-yield, high-quality, disease-resistant and highly-applicable hybrid varieties of red-rooted salvia with fine comprehensive agronomic characters, which are planted in a base of Shangluo red-rooted salvia, even in same planting areas of China, and is applicable to the technical field of breeding.

The invention relates to the technical field of agriculture bioengineering and particularly relates to a cultivation method of new strawberry species. The method includes steps of: pushing petals aside of female parent flowers in a big bud stage; pulling up all the stamens; picking male parent flowers to pollinate the female parent flowers; covering the flower heads with paper bags after pollination; removing the paper bags when receptacles begin expand after 10-15 d and bear young fruits; picking mature hybrid fruits after 30-40 days successively; cleaning seeds, air-drying; putting the seeds into a refrigerator; refrigerating at 1-3 DEG C and keeping for further use; sieving sandy soil with a sieve having a size of 1-2.5 mm, autoclaving, disinfecting and seeding; and after the seeds survive, planting seedlings having 4-5 leaves to a greenhouse, accelerating culture, and performing strain evaluation screening in fields to obtain the screened new strawberry species. By adoption of a crossbreeding method, strawberry individuals with different excellent properties are hybridized, and through genetic recombination, individuals with heterosis are obtained.

The invention discloses a molecular marker related to chicken carcass characters and an acquisition method and application thereof. The molecular marker related to the chicken carcass characters is single base mutation of a ninth exon G+1479A positioned at a fat mass and obesity associated (FTO) gene. The molecular marker is obtained by cloning full length of a chicken FTO gene by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) technology, wherein a full-length sequence and a coding region sequence of the FTO gene are shown as SEQIDNO:1-2. Correlation analysis of the chicken carcass characters shows that the carcass weight, wing weight, leg muscle weight, half eviscerate weight and full eviscerate weight of a 90-day-old mutated individual are obviously higher than those of a wild type individual.

The invention discloses an artificial pollination and breeding method of salt-resisting and fast-growing fraxinus velutina. The artificial pollination and breeding method comprises the following steps: (1) selecting male and female parents; (2) determining pollination time; (3) collecting male parent pollen; (4) storing the male parent pollen; (5) carrying out an artificial pollination procedure;(6) obtaining hybrid seeds; (7) sowing the seeds and culturing seedlings. The method disclosed by the invention is characterized by determining an optimal pollination period; the pollination is artificially controlled and is purposefully polymerized with an existing breeding method for good properties of fraxinus chinensis germplasm; the difficulty of obtaining fraxinus velutina new germplasm withthe stable and good properties including salt resistance and fast growing and the like by artificial controlled pollination is effectively overcome, and the aims of accurately and rapidly breeding the fraxinus velutina is realized. By adopting the method disclosed by the invention, a lot of excellent germplasms can be obtained within short time and requirements on high-quality germchits of the fraxinus velutina in China are met.

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention provides a new ostrea rivularis species breeding method. Firstly, ostrea rivularis and crassostrea gigas parents are selected, and oyster parent ripening promotion breeding is conducted; secondly, ostrea rivularis and crassostrea gigas are manually dissected, female ostrea rivularis and male crassostrea gigas are selected in a microscopic examination mode, and eggs of ostrea rivularis and sperms of crassostrea gigas are taken out for fertilization to form hybrid fertilized ovums of ostrea rivularis; thirdly, after incubation of the hybrid fertilized ovums, larvae are selected and bred in a larva breeding pond; finally, settlement and metamorphosis are conducted on the bred larvae, and a first generation of hybrid fry of ostrea rivularis for breeding is bred, and through artificial breeding, new ostrea rivularis species which is high in disease resistance and growing speed and good in meat quality is screened out to be bred.

The present invention discloses a method of selectively breeding, reproducing and preparing hybrid seed of Caitai cytoplasm male sterile line. The present invention adopts sterile source Chinese cabbage CMS3411-7 as female parent and Liuzhou Caitai selfed line as male parent, to breed the Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 through backcross for several generations, pair matching and strict selection. The Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 have stable sterility, normal pistil function, healthy honey cup, no yellowing in seedling stage and excellent natural pollination. The bred hybrid varieties TC1-3-1x TS14 and TC2-8 x TS46 have fast growth speed, high yield, high quality and high disease resistance.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The present invention provides a method for culturing celery hybrid species by utilizing male sterility dual-purpose line. Said method utilizes steril plant in the male sterility dual-purpose line controlled by nuclear recessive gene as female parent, and selects several celery self-bred lines as male parent to respectively prepare hybridized combinations. Besides, said method adopts the following steps: in the initial stage of flowering identifying fertilizable plants in male sterility dual-purpose line and completely removing them, after the pollination is combleted; removing all the male parent plants, and collecting seeds from female parent plants so as to obtain the hybrid seeds with 10% purity.

The invention discloses a breeding method for rapidly obtaining a cytoplasm male sterility line and a maintenance line of carmine radish. The method mainly comprises the following steps: selecting different types of carmine radish materials, performing first generation selfing, determining a selective breeding object, performing orientation selection on a single plant, and performing second generation selfing; performing orientation selection on the single plant for continuously selfing in a selfing offspring group, and performing orientation selection on the single plant according to the selective breeding object; selecting cytoplasm male sterility as a female parent, selecting the selected single plant as a male parent, performing interspecies cross, obtaining hybrid F1, thereby transferring CMS genes of the sterility to collard; continuously selfing and purifying the selfing offspring, and screening a sterile plant which is the closest to the selective breeding object of the male parent to serve as a female parent in a cytoplasm male sterility hybrid plant group, taking the screen single plant which is the closest to the selective breeding object in the selfing offspring as a male parent for continuously performing paired cross; and purifying the selfing offspring, transferring the male sterility, simultaneously performing paired cross on each generation, and selfing multiple generations to obtain the maintenance line and the male sterility line. According to the method, the labor and cost can be obviously reduced, and the breeding time is shortened.

The invention relates to a chicken breed producing method, in particular to a method for producing high survival rate san huang chicken breeds. According to the method, a yellow feather chicken is used as a first male parent of terminal lines, a high-yield high-quality san huang chicken is used as a first female parent of the terminal lines, an F1 generation is produced, hens with yellow feather, yellow feet and yellow skin are selected among the F1 generation for seed reservation to form a second female parent of the terminal lines, a local san huang chicken breed is selected as a second male parent of the terminal lines, and the local san huang chicken breed is subjected to hybridization with the F1 generation hens to obtain an F2 generation which is a high survival rate san huang chicken. The method for producing the high survival rate san huang chicken breeds has the advantages that the san huang chicken survival rate, the growing speed and the weight uniformity are increased, and hybrid vigor is remarkable.

The invention relates to a method for breeding cucumis allotriploid cucumbers, belonging to the field of biotechnological breeding. The method is special for chromosome engineering and ploidy breeding of cucumbers. Cucumis diploid new species are positively and negatively hybridized with diploid cucumbers; and the seeds harvested from the hybridized fruit are the seeds of the allotriploid cucumbers. The seedling allotriploid cucumbers have strong growth potential and excellent economic characteristic, and can serve as low-temperature-resistant, weak-light-resistant and Meloidogyne incognita Chitwood-resistant processing type greenhouse culture cucumber new species to be popularized and applied. The seedling allotriploid cucumbers provide precious materials for researching scientific problems such as cucumber cotyledon genetic development law, fruit development, parthenocarpy mechanism, heterosis mechanism and the like, facilitates utilization of the allotriploid cucumbers, and contributes to transducing wild type excellent genes to culture the cucumber new species.

The invention discloses a method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines, comprising: hybridizing a cytoplasmic male-sterile line DHCMS08-5-3-6-9 trans-bred with a Brassica oleracea DH line as a female parent and the Brassica oleracea DH line DH05X-1-5-9-8 line as a male parent to prepare DHCMS08-5-5-6-9*DH05X-1-5-9-8 hybrid first-generation combination, named as Qingan 1562. The method of the invention is a method to breed a mid-early maturity novel variety of Brassica oleracea by using two DH lines, a novel hybrid first-generation variety of Brassica oleracea is bred through DH liens with genotype purity of 100% created by culturing isolated microspores of Brassica oleracea, the novel hybrid first-generation variety of Brassica oleracea has highly uniform economic traits and completely exhibited target traits, and the breeding of disease-resistant high-quality high-yield variety breeding targets is achieved.

The invention discloses an outstanding fairy mushroom crossbreeding method which comprises the following steps that (1) a white jade mushroom bacterial strain and a gray crab mushroom bacterial strainare cultivated respectively to obtain corresponding right-age hyphae; (2) a protoplast is prepared and generated: under the aseptic condition, the right-age hyphae obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purification is performed, a protoplast regeneration culture solution is added, and sealing culture is performed; (3) a tip hyphaof the regenerated protoplast is selected for culture, a mononuclear regeneration bacterial strain is screened under the aseptic condition, then the white jade mushroom bacterial strain and the graycrab mushroom bacterial strain are subjected to pairing and crossbreeding, inoculated culture and purification are performed, and the outstanding fairy mushroom is obtained.

The invention discloses an onion full-sterile seed production method which comprises the following steps of searching a sterile plant in an onion seed production field for bagging; screening out a maintainer plant from fertile plants by using a jnurf13 molecular marker; conducting bagging and hybridizing with plant flower balls above sterile flower balls, and conducting bagging and selfing on residues; enabling the seeding time to be 28-32 days earlier than the normal onion seeding time; conducting seedling stage identification, specifically, identifying fertility by using an orf725 molecularmarker, and removing fertile seedlings; conducting mulching film planting in rain sheltering facilities; and harvesting bulbs which are not bolted to be used as seed balls for seed reproduction, and harvesting sterile seeds in time when seed coats of bolted bulbs become brown from green. The onion seeds obtained by the method are fully-sterile onion seeds, male parent maintainer lines and female parent sterile lines are directly hybridized, manual castration is not needed, manpower is greatly saved, meanwhile, the produced seeds are hybridized seeds, the heterosis is obvious, the onion yield is increased, meanwhile, fully-sterile seed balls can protect varieties against invasion, and the breeding unit variety protection cost is greatly reduced.

The invention discloses a pond cultivation method for current-year fries of half-bred takifugu, and the method is characterized in that the method comprises the steps: carrying out desilting and disinfecting of a pond before the fries are put in the pond; splashing compound fertilizer cultivation biology baits after water enters into the pond, wherein the body length of each fry needs to be greater than 8 mm, and the activity of the fries is high; enabling the water salinity of the pond to be gradually increased to the pond cultivation salinity before the fries are put in the pond, wherein the fries needs to be in water for carrying, and the cultivation density of the fries is 4500 / 1000 square meters; casting no bait at the initial stage of fry throwing; splashing soybean milk and frozen big artemias when the biology baits in the pond is not sufficient, thereby reducing the mutual killing of the fries, and improving the survival rate of the fries; casting cluster eel cooperation baits after the body length of the fries reaches 20mm; changing water one time per 15 days at the early stage of cultivation; adjusting the water-changing frequency to be one time per 7 days at the middle and later stages of cultivation; splashing a biological agent into the pond in a cultivation process, so as to prevent parasites; transferring half-bred fishes to a thermal-insulation greenhouse for overwintering when the temperature of water in the pond decreases to 18 DEG C or less, wherein the catching mode is a mode of trawling, and a knotless net is used for trawling, thereby avoiding the scratching damage to the fish body.

The invention discloses a method for breeding and producing a new variety of a commercial line of eriocheir sinensis. The method comprises the following steps: (1) parent selection of the commercial line: selecting commercial selection lines A and B of eriocheir sinensis for serving as parents; (2) production of the commercial line: performing diallel crossing on the commercial selection lines A and B to produce the commercial line; (3) screening of the commercial line: screening an A-female and B-male commercial line which takes line A as a female parent and takes line B as a male parent through growth contrast test; (4) performing productive verification on the commercial line; (5) applying the new variety of the commercial line. By adopting the new variety of the commercial line bred and produced through the method, the major advantages of the female parent A and the male parent B are well combined, high growth speed, high group yield and remarkable hybrid vigor are realized, and excellent provenance is provided for the river crab industry in China. The new variety is the first variety of the commercial line of eriocheir sinensis in China.

The invention disclose a new product of edible mushrooms. The new product of the edible mushrooms is obtained from hybridization breeding by adopting the following steps (1), a white beech mushroom bacterial strain and a gray crab mushroom bacterial strain are cultivated respectively and corresponding hypha of the right age are obtained; (2), a protoplast is prepared and regenerated, specifically,under an aseptic condition, the hypha of the right age obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purifying is conducted, a protoplast regeneration culture solution is added, and sealing culture is conducted; and (3), top end hypha of the regenerative protoplast is selected and taken to be subjected to culture, mononuclear regenerative bacterial strains are screened under an aseptic condition, then a white beech mushroom mononuclear bacterial strain and a grey cab mushroom mononuclear bacterial strain are subjected to matching hybridization, and the new product can be obtained after inoculated culture and purification are conducted.

The invention discloses a method for selecting a transport-resistant mid-maturation new variety from CMS (cytoplasmic male sterile) and DH (double haploid) series of cabbages. The method includes the steps of selecting a CMS DH09-MP01-78CMS series from the cabbages to serve as a female parent; selecting a DH DH11-52-81M3 series to serve as a male parent, and enabling the female parent and the male parent to hybridize to obtain a CMS DH09-MP01-78CMS x DH11-52-81M3 combination. The CMS DH09-MP01-78CMS x DH11-52-81M3 combination is named 'Gaoshan 1st' variety. The new variety is subjected to variety comparison testing, variety regional testing and production demonstration in main high-mountain vegetable farming areas including Fengxian County in Baoji, Taibai County and the like of Shanxi Province, proved by the tests, the new variety is remarkable in heterosis, excellent in comprehensive economic characters, mid-mature, resistance to transport, remarkable in disease resistance and yielding ability, high in adaptability and stable in product increasing, and the new variety is rarer and 9.7%-14.1% more in yield as compared with a compared cabbage variety.

The invention discloses a method of utilizing cabbage CMS and DH lines to selectively breed early-ripening new variety. The method includes following steps: selecting cabbage cytoplasm male sterile line DH08-GW100M3CMS as a female parent and DH line DH12-3-M2 as a male parent for hybridization to breed a DH08-GW100M3CMSxDH12-3-M2 combination which is named as a qin'gan 658 variety. Variety comparison experiments, variety area experiments and production demonstration of the variety in Yang Ling, Xi'an and Baoji Taibai which are major vegetable cultivation areas in Shanxi province show that the variety is remarkable in hybrid vigor, excellent in comprehensive economic character, resistant to head bursting, high in quality, disease-resistant, outstanding in yielding ability, high in adaptability and stable in yield increase advantage, and yield is increased by 10.2-14.3% when compared with that of a cabbage variety 8398.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.