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New product of edible mushrooms

A new variety, edible fungus technology, applied in the direction of fungal products, applications, fungi, etc., can solve the problems of easy-to-contaminate bacteria, poor stress resistance, long production cycle, etc.

Inactive Publication Date: 2018-03-30
JINAN PENGSHENG AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new variety of edible fungus to overcome the problems of long production cycle, easy to infect bacteria, and poor stress resistance in the existing industrial cultivation of commonly used mushroom species, and to provide a new variety of edible fungus, specifically a kind of mushroom with A New Variety of Shiji Mushroom with Excellent Performance (Cuixian Mushroom)

Method used

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Embodiment 1

[0042] A new variety of edible fungus is obtained by cross-breeding through the following methods:

[0043] (1) Cultivate the white jade mushroom strain and the gray crab mushroom strain respectively to obtain the corresponding age-appropriate mycelia:

[0044]Inoculate it into the PDA medium on the slant of the test tube, culture at 25°C for 10 days, then transfer to the plate PDA medium, culture at 25°C for 5-7 days, then transfer to a 250mL Erlenmeyer flask liquid enriched medium, at 25°C Shake culture on a constant temperature shaker for 4 days, and finally use a sterile pipette tip to pipette 5 mL of fresh young culture solution into another 250 mL Erlenmeyer flask with liquid enriched medium, and culture at a constant temperature of 25°C for 4 days.

[0045] (2) Preparation and regeneration of protoplasts: Under sterile conditions, the age-appropriate mycelia obtained in step (1) were subjected to enzymatic hydrolysis treatment to obtain protoplasts, purified, added to p...

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Abstract

The invention disclose a new product of edible mushrooms. The new product of the edible mushrooms is obtained from hybridization breeding by adopting the following steps (1), a white beech mushroom bacterial strain and a gray crab mushroom bacterial strain are cultivated respectively and corresponding hypha of the right age are obtained; (2), a protoplast is prepared and regenerated, specifically,under an aseptic condition, the hypha of the right age obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purifying is conducted, a protoplast regeneration culture solution is added, and sealing culture is conducted; and (3), top end hypha of the regenerative protoplast is selected and taken to be subjected to culture, mononuclear regenerative bacterial strains are screened under an aseptic condition, then a white beech mushroom mononuclear bacterial strain and a grey cab mushroom mononuclear bacterial strain are subjected to matching hybridization, and the new product can be obtained after inoculated culture and purification are conducted.

Description

technical field [0001] The invention relates to a new variety, in particular to a new edible fungus variety. It belongs to the technical field of edible fungi. Background technique [0002] There are a large number of edible large fungi in nature. Specifically, edible fungi generally refer to edible mushrooms, which can form large fleshy / colloid fruiting bodies or sclerotia-like tissues and can be eaten by humans. Due to the limited varieties of artificially cultivated edible fungi, the cultivation methods of known varieties and the development of new varieties have always been research hotspots in the field of edible fungi. [0003] Shiji mushroom, also known as seafood mushroom, is crisp and tender in texture, delicious in taste, rich in nutrition, and has the taste of sea crab. Since the 1980s, Shiji mushroom has been commercially planted in my country and has become an important edible fungus in the country. One of the factory products. In cultivation and production, i...

Claims

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Application Information

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IPC IPC(8): C12N15/04C12N1/14A01H15/00
CPCA01H15/00C12N15/04C12N1/145C12R2001/645
Inventor 刘克东刘振涛于秀英杨晶闫卫荣单书凯
Owner JINAN PENGSHENG AGRI TECH
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