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Method for breeding cucumis allotriploid cucumbers

A technology of triploid and cucumber, which is applied in the direction of botanical equipment and methods, applications, plant gene improvement, etc., can solve the problem of low affinity and cannot obtain viable seeds, and achieve neat fruit shape, high germination rate, and strong growth potential Effect

Inactive Publication Date: 2011-10-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0007] The present invention is aimed at the process of creating an allotriploid by crossing a new double diploid species of the genus Melon with a diploid cucumber. In order to solve the problem of energetic seeds, a method of breeding new species of allotriploid cucumbers of the genus Melon, which is hybridized with diploid cucumbers to produce vigorous seeds, was proposed, which further explored the utilization value of allotriploids and promoted the development of allotriploid Popularization and Application of Ploid Cucumber

Method used

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  • Method for breeding cucumis allotriploid cucumbers

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Embodiment 1

[0023] Diploid new species of Melon and common diploid cucumber P01, P02, P03, CC1, CC4, EC1, Hazerd, 627, Changchun dense thorn, Pingwang cucumber, Xishuangbanna cucumber for seed soaking, germination, seeding and planting And normal plant management, and new planting should be carried out 1 month in advance to make the new plant meet the flowering period of ordinary diploid cucumber.

[0024] The new diploid species of the genus Muskmelon is obtained by crossing the wild species of the genus Muskmelon and the common cultivated cucumber through somatic clone mutation (Chen Jinfeng, Zhuang Feiyun, Qian Chuntao. A new species of the genus Muskmelon (double diploid) was synthesized and Qualitative[J].Wuhan Botany Research, 2001,19(5):357-362); P01 was selected from 6 generations self-bred of Jinchun 5 cucumber variety (Zhang Xiaoqing. Creation of Cucumber Autotetraploid And related research on different ploidy materials [D]. Nanjing: Nanjing Agricultural University, 2007: 16); P02 ...

Embodiment 2

[0032] Common diploid cucumber Changchun dense thorn, Pingwang small cucumber, Xintai dense thorn, Youjia whole female 09, 7011A, HH1-8-57, HH1-8-1-2, CC1, EC1, P03 and Melon Diploid new species should be soaked, germinated, planted, planted, and normal plant management, and the new species should be carried out 1 month in advance so that the flowering period of the two will meet.

[0033] Changchun dense thorn is a local variety of Changchun City (Luo Xiangdong. Cultivated cucumber ( Cucumis sativus L.) and pickled cucumber ( C. hystrix Chakr.) interspecific hybrids and the creation and evaluation of hetero-staining systems[D]. Nanjing: Nanjing Agricultural University, 2006: 32), Pingwang cucumber is a local farm variety (Pu Yemin. Pingwang cucumber[J]. Jiangsu Agricultural Sciences, 1985, (7): 13); Xintai Misi is an excellent local variety (Cao Qinghe, Chen Jinfeng, Li Ying, etc. Cucumber-pickled cucumber introgressive system downy mildew resistance and several enzymes befo...

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Abstract

The invention relates to a method for breeding cucumis allotriploid cucumbers, belonging to the field of biotechnological breeding. The method is special for chromosome engineering and ploidy breeding of cucumbers. Cucumis diploid new species are positively and negatively hybridized with diploid cucumbers; and the seeds harvested from the hybridized fruit are the seeds of the allotriploid cucumbers. The seedling allotriploid cucumbers have strong growth potential and excellent economic characteristic, and can serve as low-temperature-resistant, weak-light-resistant and Meloidogyne incognita Chitwood-resistant processing type greenhouse culture cucumber new species to be popularized and applied. The seedling allotriploid cucumbers provide precious materials for researching scientific problems such as cucumber cotyledon genetic development law, fruit development, parthenocarpy mechanism, heterosis mechanism and the like, facilitates utilization of the allotriploid cucumbers, and contributes to transducing wild type excellent genes to culture the cucumber new species.

Description

1. Technical Field [0001] The invention relates to a breeding method for allotriploid cucumbers of the genus Muskmelon, which belongs to the field of biotechnology breeding and is specially used for chromosome engineering and ploidy breeding of cucumbers. 2. Background technology [0002] In the patent "Method for Synthesis of New Species and Cucumber Breeding from Wild Species of Melon" (Application No. 00112271.1) applied by our research group, we have overcome the gap between wild species and cultivated species of Melon through immature embryo rescue. Inter-hybridization obstacles, and the use of somatic clonal mutation to successfully double the chromosomes of double-haploid inter-species hybrids. After fertility restoration screening, the world's first inter-species hybrid double diploid of Muskmelon was cultivated. New species C. hytivus . [0003] Allotriploids are obtained by crossing new diploid species with cultivated cucumbers, and have a set of wild species C. hytrix ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈劲枫张淑霞陈龙正娄群峰钱春桃
Owner NANJING AGRICULTURAL UNIVERSITY
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