Onion full-sterile seed production method

Abstract

The invention discloses an onion full-sterile seed production method which comprises the following steps of searching a sterile plant in an onion seed production field for bagging; screening out a maintainer plant from fertile plants by using a jnurf13 molecular marker; conducting bagging and hybridizing with plant flower balls above sterile flower balls, and conducting bagging and selfing on residues; enabling the seeding time to be 28-32 days earlier than the normal onion seeding time; conducting seedling stage identification, specifically, identifying fertility by using an orf725 molecularmarker, and removing fertile seedlings; conducting mulching film planting in rain sheltering facilities; and harvesting bulbs which are not bolted to be used as seed balls for seed reproduction, and harvesting sterile seeds in time when seed coats of bolted bulbs become brown from green. The onion seeds obtained by the method are fully-sterile onion seeds, male parent maintainer lines and female parent sterile lines are directly hybridized, manual castration is not needed, manpower is greatly saved, meanwhile, the produced seeds are hybridized seeds, the heterosis is obvious, the onion yield is increased, meanwhile, fully-sterile seed balls can protect varieties against invasion, and the breeding unit variety protection cost is greatly reduced.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to methods for creating vegetable male sterile lines and utilizing heterosis, in particular to a breeding process for onion male sterile lines and a method for producing total sterile seeds using the male sterile lines. Background technique [0002] Onion (Allium cepa L.) is a biennial herb whose product organs are composed of fleshy scales and scale buds in the Allium family (Alliaceae). Onion production areas often use self-reserved seeds for planting, which leads to year-by-year degradation of varieties, poor consistency of bulbs, and low yield. Using sterile lines of the same variety to carry out total sterile seed production can not only prevent the production of self-reserved seeds, but also increase production. [0003] In onion seed production fields, the frequency of sterile plants is 0.1%-24%, it is easy to find sterile plants, and it is relatively easy to select sterile lines. ...

AI Technical Summary

Problems solved by technology

Since it takes three years from onion seeds to the bulbs, and the bulbs bolt and bloom, the breeding period cannot be shortened. The traditional method of breeding maintainers is relatively difficult, and it is difficult to realize the total sterile seed production of this variety.