Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish

Abstract

The invention discloses a breeding method for rapidly obtaining a cytoplasm male sterility line and a maintenance line of carmine radish. The method mainly comprises the following steps: selecting different types of carmine radish materials, performing first generation selfing, determining a selective breeding object, performing orientation selection on a single plant, and performing second generation selfing; performing orientation selection on the single plant for continuously selfing in a selfing offspring group, and performing orientation selection on the single plant according to the selective breeding object; selecting cytoplasm male sterility as a female parent, selecting the selected single plant as a male parent, performing interspecies cross, obtaining hybrid F1, thereby transferring CMS genes of the sterility to collard; continuously selfing and purifying the selfing offspring, and screening a sterile plant which is the closest to the selective breeding object of the male parent to serve as a female parent in a cytoplasm male sterility hybrid plant group, taking the screen single plant which is the closest to the selective breeding object in the selfing offspring as a male parent for continuously performing paired cross; and purifying the selfing offspring, transferring the male sterility, simultaneously performing paired cross on each generation, and selfing multiple generations to obtain the maintenance line and the male sterility line. According to the method, the labor and cost can be obviously reduced, and the breeding time is shortened.

Description

technical field [0001] The invention relates to a breeding method of rouge radish, in particular to a breeding method for quickly obtaining a cytoplasmic male sterile line and a maintainer line of rouge radish, and belongs to the technical field of plant breeding. Background technique [0002] Rouge radish (Raphanus Sativus L.Sinoruber Makino), also known as red radish, belongs to a local variety of radish in the family Brassicaceae. It is famous for its red carpel and rich in anthocyanins. It is mainly produced in Fuling District, Chongqing City, near It is also cultivated in cities and counties, Sichuan, Guizhou and other provinces. In recent years, many units have extracted natural anthocyanins from the fleshy root of rouge radish. Radish red pigment is a kind of natural food coloring, which is safe, non-toxic, rich in resources, and has certain nutritional and pharmacological effects. It is one of the natural pigments allowed to be used by the National Additive Standard...

AI Technical Summary

Problems solved by technology

Lu Fafa et al. (Chinese Vegetables, 2012(2): 104-106) and Tao Hongying et al. (Chinese Vegetables, 2012(1): 37) respectively prepared Rouge 1 and Rouge 2 Red Heart by using the male sterile lines selected. Radish hybrids, but the selection process for male sterile lines and maintainer lines is not elaborated

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