Avian adenovirus 4, 8 and 11 type trivalent vaccine as well as preparation method and application thereof

An avian adenovirus and vaccine technology, which is applied in biochemical equipment and methods, antiviral agents, viruses/phages, etc., can solve the problems of weak cross protection of avian adenoviruses and different avian adenoviruses, etc., and achieve stable immune effect and immunity. The effect of good originality and high virus titer

Pending Publication Date: 2022-04-26
乾元浩生物股份有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] Although pericardial effusion-hepatitis syndrome and inclusion body hepatitis are very similar in histopathological changes, the avian adenoviruses that cause the two diseases are not the same, and there are also certain differences in the prevalence and lesion characteristics, and avian adenoviruses Cross-protection between different serotypes is relatively weak

Method used

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  • Avian adenovirus 4, 8 and 11 type trivalent vaccine as well as preparation method and application thereof
  • Avian adenovirus 4, 8 and 11 type trivalent vaccine as well as preparation method and application thereof
  • Avian adenovirus 4, 8 and 11 type trivalent vaccine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation and screening of wild strains of avian adenovirus

[0040] 1. Isolation and identification of wild strains of avian adenovirus

[0041] The present invention collects multiple suspected poultry adenovirus-infected tissue samples in Liaoning, Hebei, Heilongjiang, Shandong, Henan, Jiangsu, Yunnan and Ningxia, etc., and the main apparent necropsy lesions of sick chickens: (1) Accumulation in the pericardial cavity can be seen About 5-10mL of light yellow clear water sample or jelly-like liquid; (2) The liver fades to light brown to yellow, brittle and brittle, swollen and fatty degeneration, the liver color is lighter, and there are local necrotic lesions , Kidney pale, swollen, hemorrhage etc.

[0042]The present invention collects the liver tissue with typical symptoms and pathological changes, adds sterilized PBS, shreds and grinds it according to the weight ratio of 1:3 after weighing, centrifuges at 12000r / min at 4°C for 10min, and passes through...

Embodiment 2

[0057] Example 2: Identification and properties of QYH2019-YN strain, QYH2020-HB strain and QYH2020-SY strain

[0058] 1. Sterility test

[0059] Tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", the F1 generation seed virus samples of QYH2019-YN strain, QYH2020-HB strain and QYH2020-SY strain were inoculated and grew aseptically.

[0060] 2. Determination of virus content

[0061] Dilute the F1 generation virus solution of QYH2019-YN strain, QYH2020-HB strain and QYH2020-SY strain 10 times serially to 10 -10 , take 10 -5 -10 -10 Four dilutions were inoculated into 96-well culture plates with good growth of LMH cells, and placed at 37°C, 5% CO 2 After 2 hours of incubation, maintenance solution was added and culture continued for 168 hours. After 168 hours, the cell lesions were observed hole by hole, and the typical lesions of the inoculated cells were judged as infection, and the virus content was calculated according to the Reed-Muench...

Embodiment 3

[0080] Example 3: Optimum inoculation amount and harvest time of QYH2019-YN strain, QYH2020-HB strain and QYH2020-SY strain

[0081] 1. Optimal inoculation volume

[0082] The seed viruses of QYH2019-YN strain, QYH2020-HB strain and QYH2020-SY strain were inoculated on well-growing monolayer LMH cells according to 1:100, 1:1000 and 1:10000 respectively, and each sample was repeated 3 times, and placed at 37 °C, 5% CO 2 Cultivate and observe cell lesions. When more than 80-90% of the cells are lesions, harvest the virus solution. After repeated freezing and thawing twice, use the real-time PCR method to measure the virus particles in the culture, and select the one with the highest concentration to determine the optimal inoculation dose. . The results showed that: QYH2019-YN strain was inoculated at 1:1000, QYH2020-HB strain and QYH2020-SY strain were inoculated at 1:100, and the virus titer was the highest.

[0083] 2. The best harvest time

[0084] According to the best i...

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Abstract

The invention relates to the technical field of veterinary biological products, in particular to an avian adenovirus 4, 8 and 11 type trivalent vaccine as well as a preparation method and application thereof. The fowl adenovirus trivalent vaccine comprises a fowl adenovirus type 4 vaccine strain QYH2019-YN, a fowl adenovirus type 8 vaccine strain QYH2020-HB and a fowl adenovirus type 11 vaccine strain QYH2020-SY. The invention provides a fowl adenovirus trivalent vaccine which is prepared from a fowl adenovirus type 4 vaccine strain, a fowl adenovirus type 8 vaccine strain and a fowl adenovirus type 11 vaccine strain which are obtained by newest separation, and has an effective prevention effect on fowl adenovirus type 4, fowl adenovirus type 8 and fowl adenovirus type 11 as well as chicken inclusion body hepatitis and hydropericardium-hepatitis syndrome caused by the fowl adenovirus type 4, fowl adenovirus type 8 and fowl adenovirus type 11.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an avian adenovirus type 4, 8 and 11 trivalent vaccine and its preparation method and application. Background technique [0002] Fowl adenovirus (FAV-I) belongs to the family Adenoviridae and belongs to the genus Fowl Adenovirus. Avian adenoviruses are common pathogens of infectious diseases and widely exist in the respiratory and digestive tracts of various birds. Only a few adenoviruses are pathogenic, and most adenoviruses can replicate in birds, but do not show clinical signs or symptoms Very mild, in the case of mixed infection, adenovirus can become a conditional pathogen, which can cause recessive infection in chicken flocks or cause morbidity and death in chicken flocks as a secondary pathogen. [0003] Avian adenoviruses are divided into three subgroups I, II, and III according to group-specific antigens. Currently, there are 5 species and 12 sero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/295A61K39/235A61P31/20C12R1/93
CPCC12N7/00A61K39/12A61P31/20C12N2710/10221C12N2710/10234A61K2039/70A61K2039/552A61K2039/5252A61K2039/54
Inventor 王增福李跃吴宗学孙雪史张艳赵成全程海波李海鹰倪娇
Owner 乾元浩生物股份有限公司
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