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Fowl adenovirus group I immunity related fusion protein as well as encoding gene and application thereof

A technology for fusion proteins and coding genes, which can be used in gene therapy, antiviral agents, hybrid peptides, etc., and can solve problems such as threats to the poultry industry

Active Publication Date: 2012-07-25
GUANGXI VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, group I poultry adenoviruses have caused diseases such as chicken inclusion body hepatitis, pericardial effusion hepatitis syndrome, and gizzard erosion. Although group I poultry adenoviruses are often negatively infected, they act together with other pathogens on poultry. threat to the poultry industry

Method used

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  • Fowl adenovirus group I immunity related fusion protein as well as encoding gene and application thereof
  • Fowl adenovirus group I immunity related fusion protein as well as encoding gene and application thereof
  • Fowl adenovirus group I immunity related fusion protein as well as encoding gene and application thereof

Examples

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Effect test

Embodiment 1

[0046] Example 1. Obtaining of DNA Fragments Containing Hexon-encoding Genes and chIL2-Encoding Genes

[0047] 1. Acquisition of Hexon-encoding gene and chIL2-encoding gene

[0048] According to the Hexon gene sequence and the gene sequence of chIL2 of group I fowl adenovirus (fowl adenovirus group I, FAVI), utilize the software primerprimer5.0 to design the primers Ha, Hb of Hexon gene and the primers chIL2a, chIL2b of chIL2 gene, underline is linker sequence. Primers were synthesized by Shanghai Invitrogen Company.

[0049] The primer sequences are shown in Table 1:

[0050] Table 1 Primer sequences

[0051]

[0052] 1. Acquisition of chIL-2 coding gene

[0053]Using the recombinant plasmid PMD-18T-IL-2 (the 2825-3265 nucleotides from the 5' end of the sequence 5 in the synthetic sequence table, connected to the PMD-18T vector.) as a template, add TaqDNA polymerase, primers chIL2a and chIL2b , dNTPs for PCR reaction, the reaction system is: 10×PCR Buffer 5ul, 2.5umol...

Embodiment 2

[0079] Example 2, the application of the DNA fragment containing the coding gene of Hexon and the coding gene of chIL2

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Abstract

The invention discloses a fowl adenovirus group I immunity related fusion protein as well as an encoding gene and application of the fowl adenovirus group I immunity related fusion protein. The fusion protein provided by the invention comprises Hexon protein and chicken interleukin-2. The Hexon protein is the protein composed of an amino acid sequence from the 1st position to the 914th position of N' tail end of the sequence 6 in a sequence table, and the chicken interleukin-2 is the protein composed of the amino acid sequence from the 933rd position to the 1075th position of the N' tail end of the sequence 6 in the sequence table. Experiments show that a DNA (deoxyribonucleic acid) molecule composed of a Hexon gene and a chicken interleukin-2 (chIL-2) gene, which contain fowl adenovirus group I and are connected in series, is provided, a Hexon specific antibody can be generated when the DNA molecule is injected to chicken muscle by an expression vector, and the DNA molecule has good immunity protection effect and can be used for effectively preventing the fowl adenovirus group I after fowl adenovirus group I challenge experiments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune-related fusion protein with group I poultry adenovirus, its coding gene and application. Background technique [0002] Fowl adenovirus group I (FAVI) includes a total of 12 serotypes of viruses isolated from chickens, turkeys, geese and other birds, among which the more famous virus strains include chicken embryo lethal orphan virus, quail bronchitis Virus, chicken inclusion body hepatitis virus. In recent years, group I poultry adenoviruses have caused diseases such as chicken inclusion body hepatitis, pericardial effusion hepatitis syndrome, and gizzard erosion. Although group I poultry adenoviruses are often negatively infected, they act together with other pathogens on poultry. pose a greater threat to the poultry industry. [0003] Compared with traditional vaccines, the production of genetically engineered vaccines is completely carried out in vitro, and there is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A61K39/235A61K39/39A61K48/00A61P31/20
Inventor 谢芝勋谢丽基唐熠谢志勤庞耀珊邓显文刘加波范晴
Owner GUANGXI VETERINARY RES INST
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