DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device

Abstract

The present invention disclosed the use of single nucleotide polymorphism (SNP) as the detection assay for human identification. Using the reversed dot-blot format and the flow through hybridization process, the process can be more efficient, less expensive and with similar or better power of exclusion in definitive identification. The present method can be applied to any other organisms.

Description

CROSS REFERENCE [0001] This is a regular application of a provisional application, application No. 60 / 346,133, filed on Nov. 7, 2001.BACKGROUND OF THE PRESENT INVENTION [0002] 1. Field of Invention [0003] The present invention relates to method of making definitive identification of a human or any organism by DNA analysis and the device thereof. [0004] 2. Description of Related Arts [0005] DNA fingerprinting by RFLP first introduced in 1985 (Gill P, Jeffreys A J, Werrett D J) for human identification was subsequently applied to other organisms. In human it was widely accepted as the best forensic tool for identification of suspects in criminal cases, paternity disputes and often used as the distinct human ID code. Recently the relatively time consuming RFLP method is mostly replaced by the high throughput automation processes. Using PCR amplification of analyzing the number of short tandem repeat (STR), first discovered in 1991 (Edwards A, Civitello A, Hammond H A, Caskey C T), from...

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Benefits of technology

[0006] Now that the human genome and many other organisms have been sequenced and mapped. Although within any species the general DNA sequencing information is very similar but each and every one has each own distinct sets of information. Hence many scientists try to characterize disease-related variation among populations. Anthropologists use genetic variation to reconstruct our species' history, and to understand the role of culture and geography in the global distribution of human variation. Single nucleotide polymorphism (SNP) data can service these purpose (Weiss K M 1998). Indeed SNP can be used for genotyping (Brightwell G, Wycherley R, Waghom A. 2002). Hence with the use of allele specific oligonucleotide (ASO)-arrays, the number of SNP to provide adequate discriminating power is easily attainable. We use our membrane-based semi-array ASO-RDB Flow-Through hybridization format to achieve such goal (see U.S. Pat. No. 5,741,647 for details). In principle we could use the SNP of sufficient number anywhere in the genome for discriminating purpose. Ho

Problems solved by technology

However, this may compromises the accuracy of paternity and kinship analyses because of the variability of mutation rate in different part of the genome.

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