60 results about "Sea anemone" patented technology

The invention provides a method for extracting ceramide from sea anemone, which comprises the following steps: crushing, extracting, leaching, adsorbing, eluting, drying by evaporation, recrystallizing and the like to obtain the high-purity ceramide. The method is simple to operate and easy for scale-up production.

The invention relates to a preparing method of sea anemone crude extract. The preparing method includes the steps of 1, washing bred live sea anemones in pure water, placing the washed sea anemones in a beaker, adding pure water into the beaker according to a sea anemone to pure water ratio being 1:1.5 to 1:2, and performing freeze thawing twice to three times at -60 DEG C; 2, moderately unfreezing the sea anemones obtained in the step 1, and performing coarse filtration; 3, centrifuging filtrate obtained in the step 2 at 4 DEG C to 6 DEG C at the speed of 12000-14000 r / min for 10 to 15 min, and collecting supernate to obtain sea anemone crude venom; 4, subjecting the sea anemone crude venom obtained in the step 3 to fractional precipitation with acetone, centrifuging precipitate obtained in each step for 15min, collecting final precipitate, and freeze-drying the final precipitate to obtain sea anemone crude toxin. The invention further provides application of the sea anemone crude toxin in the preparation of anti-lung cancer medicaments.

The invention discloses a preparation method for sea anemone polysaccharide. The preparation method comprises the following steps: crushing raw materials by adopting a constant-temperature high-speed shearing method; performing water extraction on the crushed raw materials; performing three-level alcohol precipitation of 30 percent, 60 percent and 80 percent; then performing enzymolysis by using papain and deproteinization by using sevag to obtain three kinds of sea anemone polysaccharide containing higher saccharide content. The three kinds of sea anemone polysaccharide prepared through the preparation method disclosed by the invention can be used for preparing tumor-resisting medicines and particularly can be used for preparing medicines for resisting lung cancer, prostatic cancer or ascitic tumor.

The invention discloses a preparation method of sea anemone low molecular polypeptides. The preparation method comprises the following steps: immersing and cleaning sea anemones, crushing the sea anemones by using a crusher, adding to an industrial grade colloid mill, carrying out ultramicro crushing to a particle size of 30-50mum, uniformly mixing with water having a volume 2-8 times the volume of obtained particles, adding to a centrifuge, continuously centrifuging at 8000-12000rpm for 30min, removing the obtained supernatant, adding the obtained precipitate and water having a volume 3-5 times the volume of the obtained precipitate to a biological enzymatic hydrolysis pot, carrying out enzymatic hydrolysis under conditions comprising a temperature of 37-65DEG C and a pH value of 7.5-8.0 for 2-8h, processing the obtained enzymatic hydrolysis product by a 3KD ultrafilter membrane, carrying out nanofiltration membrane concentration of the part having a molecular weight below 3KD in the product, and carrying out spray drying to obtain the finally-finished sea anemone low molecular polypeptides, wherein the addition amount of a composite enzyme in the above enzymatic hydrolysis reaction is 0.1-3% of the amount of a raw material. The part having a molecular weight above 3KD in the enzymatic hydrolysis product returns to the biological enzymatic hydrolysis pot for enzymatic hydrolysis.

The invention provides instant sea anemone food which can be instantly edible with convenient transportation and storage. The following raw materials in weight ratio are cooked in water, sterilized and vacuum packaged into the food: peeled sea anemone, ginger, garlic, cassia bark, star aniseed, brown sugar, Chinese wolfberry, chili, salt and rice wine. The invention also provides a processing method for the instant seat anemone food. The instant sea anemone food is tasty.

The invention provides a preparation method and application of sea anemone polypeptide toxin, and the preparation method comprises the following steps: 1, extraction of sea anemone crude venom: after live sea anemone is hungry, extracting an aqueous solution containing sea anemone crude toxin by using a manual extrusion method or / and an electrical stimulation method, freeze-drying the aqueous solution containing sea anemone crude toxin, and dialyzing and desalting by using a biological CE membrane dialysis bag; 2, filtering sea anemone crude toxin molecules: centrifuging the extracted sea anemone crude toxin by using an ultrafiltration tube to obtain a filtered component; and 3, purification of the sea anemone toxin: filtering the filtered component obtained in the step 2 through a microfiltration membrane, carrying out gradient elution through a reversed-phase high performance liquid chromatograph to obtain F1-F3 components, collecting the F1 component, and carrying out freeze drying to obtain the purified sea anemone polypeptide toxin, The sea anemone polypeptide toxin prepared by the invention not only can inhibit the activity of colon cancer cells HCT-116, but also can promote the apoptosis of the colon cancer cells HCT-116.

The invention discloses a method for breeding clownfish in a subtropical zone. The method comprises a daily breeding method and a reproduction period breeding method. The daily breeding method comprises the following steps that firstly, breeding water is prepared, wherein the temperature is 25-27 DEG C, the salinity is 29-31%o, and the pH value is 7.6-8.4; secondly, a breeding environment is prepared, wherein sea anemone or corallite is placed in the water, and sufficient light is guaranteed; thirdly, bait is prepared, wherein the bait comprises shrimp meat, Spanish mackerel meat, opossum shrimps or brine shrimps, and the bait is used for feeding the clownfish in a matched mode. The reproduction period breeding method comprises the steps that objects are placed in the water to provide attachment points for fish eggs, and the light shielding state is guaranteed; parent fishes are selected, wherein the largest one in a population is a female, and strong individuals with more contact withthe female are selected as males; bait is prepared, wherein the bait comprises shrimp meat, Spanish mackerel meat, opossum shrimps or brine shrimps, and the bait is used for feeding the clownfish ina matched mode. The clownfish breeding method suitable for the subtropical zone is formed, and the survival rate and reproduction rate of the clownfish are increased.

A method for sea anemone culture in a pond is characterized in that a net cage suspended under the water surface of the pond is used for artificial culture. The net cage is shaped like a cuboid with dimensions of 2*1*1m, the upper face of the net cage is opened, the bottom face of the net cage and the surrounding side faces are fenced by net plates, and the net size is eight meshes; the multiple net cages are disposed and arranged in a line along the length direction of the net cages, the upper edge of each net cage is connected by two parallel main ropes, multiple supporting rods are fixed at an interval between the two main ropes, and a floating ball is connected at the joint between each supporting rod and the main rope; auxiliary ropes are connected to two end heads of the main rope, and the end head of each auxiliary rope is connected to a rope anchor located at the bottom of the pond; and an attaching baseplate is disposed in the net cage, the attaching baseplate comprises a bottom plate, a corrugated plate is fixed on the bottom plate, and through holes are arranged in the corrugated plate. Materials could be obtained easily; the method is simple; operations are easy; management is easy; sea anemones could be bred separately or bred together with trepang to eat trepang enemies such as copepod and gammarid; space in a pond water area is fully used; the survival rate is high; and the growth speed is high.

The invention relates to actinocongestin gene Sr1 and its coded protein, and also the uses of the said protein in preparation of drugs for cardiovascular disease. The invention employs RT-PCR method, extracts sequence 1 from total RNA of tentacles of sagartia rosea ( sequence 1 indicates) the protein coded by the gene( sequence 2 indicate) Recombinant actinocongestin protein in the invention has cardiac and anti-arrhythmia action, can prepare the cardiovascular disease drugs.

The invention provides a method for preparing an instant food. According to the method, an instant finished product is prepared from kelp, sea anemone and other marine plants serving as raw materials by the steps of washing, cooking to be tasty, chopping for modeling, freeze-drying and dehydrating in vacuum to fix the shape, post-treating and the like, and can be manufactured into multiple types. The instant food is a novel health product with unique modeling and rich taste, is advantageous to popularization and promotion, has nutritional values, is convenient for a consumer to eat, and has relatively high economic and nutritional values.

The present invention proposes a method for the industrialization of crude and pure toxins in live sea anemones, which uses the methods of starch coagulation preparation, coating, toxin collection, enzymolysis, centrifugation, nickel column chromatography, and ultrafiltration to remove impurities to obtain sea anemone protein toxoids Crude pure liquid. Compared with the prior art, the beneficial effect of the present invention is: without using protease or proteolytic agent, there are many types of sea anemone protein toxoids obtained; only sea anemone toxic substances are collected without collecting sea anemone tentacles, and the Sea anemone has little damage, its regeneration time is short, and its production sustainability is extremely advantageous, which provides conditions for the future industrial use of sea anemone toxins; the collected sea anemone toxins are less likely to be polluted by external proteins, and the protein isolated from the preliminary purification is only Create a good foundation for further purification of sea anemone toxin protein.

The invention relates to the technical field of marine microorganisms and particularly relates to a method for preparing congestin through the fermentation of marine bacteria. The method comprises thefollowing steps: (1) preparing bottle-shaking seed liquid; (2) preparing seed liquid; (3) carrying out fermentation cultivation; (4) extracting and purifying fermentation liquid; and (5) purifying acrude extract: carrying out chromatography elution, collection and rotary evaporation on a prepared liquid phase, so as to obtain white powder, namely congestin. According to the method, congestin isproduced through the fermentation of a marine bacteria strain Nioella sp.LZ7-4, so that the fermentation time is short and is only 36-40 hours, and the energy consumption can be effectively saved; theyield is relatively high and can reach over 38ug / L; and the method is simple in steps, low in workload and relatively short in period, and adopted reagents and materials are common reagents for microbial fermentation tests, harmless to a human body and environment-friendly, so that the method has relatively high market prospects and economic values.

The invention provides an epoxide sterol composition comprising epoxide sterol A and epoxide sterol B, of which the total content is greater than 95%. The epoxide sterol composition is prepared by percolation extraction, concentration and column chromatography isolation purification of a marine animal, namely green sea anemone. Experimental results show that the epoxide sterol composition (ESC) remarkably refrains the growth of rat glioma cells C6 and human glioma cells U251, induces tumor cell apoptosis or necrosis, and can be used for preparing medicines for curing brain gliomas.

The invention relates to the technical field of marine organisms, in particular to a recombinant expression method of actinoporins proteins. Actinoporins are of proteins with the molecular weight of 20kDa and have very extensive physiological activity. At present, a main method for getting actinocongestin proteins is realized by separation and purification of sea anemone tentacle proteins. The invention provides the recombinant expression method of the actinoporin proteins, and the actinoporin proteins obtained by the recombinant expression method disclosed by the invention have the advantages of biological activity and high purity. The invention further discloses application of the actinoporin proteins in preparation of medicaments for preventing or treating tumors.

The invention belongs to the technical field of fungus extraction compounds, and particularly relates to sea anemone co-epiphytic fungus SYSU-MS5127, a fermentation compound and application of the seaanemone co-epiphytic fungus and the compound. The sea anemone co-epiphytic fungus (Fusarium graminearum) strain SYSU-MS5127 can metabolize and synthesize the compound Fusarielin M; through experimental verification, the compound Fusarielin M can reduce the expression of inflammatory factors NO and PGE 2 in inflammatory macrophages and has remarkable anti-inflammatory activity; and experiments prove that thecompound Fusarielin M is free of cytotoxicity and high in safety, and has good application prospects in the aspect of preparing and developing anti-inflammatory medicines.

The invention relates to the technical field of marine organisms, aims at the problem of the technological gap of a rapid screening method of microorganisms capable of producing sea anemone toxins, and discloses a rapid screening method of microorganisms capable of producing sea anemone toxins. The rapid screening method comprises the following steps: (1) acquiring bacterial strain DNA; (2) obtaining DNA probe hybridization solution, i.e., after placing digoxin labeled DNA probe solution into boiling water, placing the digoxin labeled DNA probe solution into an ice bath, and taking the DNA probe solution to add into hybridization solution; (3) carrying out hybridization, i.e., placing a DNA membrane into the DNA probe hybridization solution, carrying out incubation and hybridization, and placing the hybridized DNA membrane into a container containing a 2*SSC solution for washing; (4) developing; (5) carrying out result determination. The DNA probe and bacterial colony in-situ hybridization method is used for rapid screening of microbial strains capable of producing the sea anemone toxins; the strain capable of producing the sea anemone toxins are accurately screened out through theDNA probes subjected to specific amplification and simple and ordered operation steps; and the method is simple, efficient, short in screening period and high in accuracy.

The invention discloses a modified product of reconstructed sea-anemone neurotoxin rhk2a, a modifying method and an application thereof. The modified product of the reconstructed sea-anemone neurotoxin rhk2a is prepared by connecting a polyglycol chain with single site specifically at an amino N end of the reconstructed sea-anemone neurotoxin rhk2a. The modified product has good equality, simple separation and purification technologies and high activated coefficient of recovery, and polyglycol site-modified sea-anemone neurotoxin rhk2a has extremely good application prospects in an aspect of treating congestive heart failure.

The invention provides a sea anemone breeding device. The device is a flexible belt, depressions are formed along the belt, and the depressions (a) are located on the cylindrical surface, at the same side, of the flexible belt (1); in other word, after the flexible belt is laid on the ground, the depressions are located on the side facing upwards at the same time. According to the method, the flexible belt is laid at the bottom of a pool, a young sea anemone is placed at the midpoint of each depression and temporarily reared in the pool, and the young sea anemones are transferred into a sea area to be suspended on a floating body to be cultivated after being adsorbed onto the depressions. According to the sea anemone breeding device and method, the flexible belt serves as the breeding substratum and can be adapted to influences of various hydraulic drags in the ocean, and the young sea anemones in the depressions can not climb out or turn out easily due to the restriction of the shape of the substratum. Compared with the prior art, the device and method have the advantage that sea anemone breeding can be conducted in the ocean so that more products with higher quality can be provided in the market.

The invention relates to a sea anemone polypeptide toxin Ap-GT, and a preparation method and application thereof. The sea anemone polypeptide toxin Ap-GT is synthesized and has a more remarkable insecticidal effect for tenebrio molitor, and a half lethal dose is 14.8 nM. The sea anemone polypeptide toxin Ap-GT with a specific sequence is synthesized, the addition amount of the sea anemone polypeptide toxin Ap-GT can be effectively controlled in application, the effect of the sea anemone polypeptide toxin Ap-GT can be exerted to the maximum extent, the dosage of a medicament is reduced, and the safety is improved to the maximum extent. The marine polypeptide which has an efficient insecticidal effect and small toxicity to mammals is obtained, and a foundation can be laid for research and development of novel, efficient and safe biological insecticides.

The invention relates to actinocongestin gene Sr6 and its coded protein, and also the uses of the said protein in preparation of drugs for cardiovascular disease. The invention employs RT-PCR method, extracts sequence 1 from total RNA of tentacles of sagartia rosea ( sequence 1 indicates) the protein coded by the gene( sequence 2 indicates) Recombinant actinocongestin protein in the invention has cardiac and anti-arrhythmia action, can prepare the cardiovascular disease drugs.

The invention relates to actinocongestin gene Sr5 and its coded protein, and also the uses of the said protein in preparation of drugs for cardiovascular disease. The invention employs RT-PCR method, extracts sequence 1 from total RNA of tentacles of sagartia rosea ( sequence 1 indicates) the protein coded by the gene( sequence 2 indicates) Recombinant actinocongestin protein in the invention has cardiac and anti-arrhythmia action, can prepare the cardiovascular disease drugs.

The invention discloses a preparation method of a sea anemone wine. The method comprises the following steps: (a) selecting Cereus sinensis which is healthy, strong in activity and free of damage, washing with disinfected seawater, and temporarily breeding in the disinfected seawater with a temperature of 18-30 DEG C for 72 hours, so that the Cereus sinensis can fully discharge digestion residues in intestines; (b) taking out the clean Cereus sinensis, putting into a water tank filled with a solid-type liquid, staying for 20-30 minutes, and taking out the Cereus sinensis when tentacles of the Cereus sinensis have no spontaneous reaction if touched; (c) rinsing with clean water for the Cereus sinensis with fixed shapes, draining, and immersing with high-degree grain liquor for half a month; and (d) taking the Cereus sinensis immersed by the liquor, washing away skin of the Cereus sinensis with clean water, filtering the high-degree grain liquor which have immersed the Cereus sinensis, filling into a container, adding the treated Cereus sinensis, shaping, sealing, and preserving at cool and dark places. The prepared sea anemone wine has no residual toxins, the sea anemone in the wine is good in shape, and the wine can be used for blood pressure lowering and blood clotting, has analgesic effects on cardiac nerves and muscles, as well as medicinal values for tranquillize and calming, pain relieving, aging resistance and cancer resistance.

The invention relates to actinocongestin gene Sr3 and its coded protein, and also the uses of the said protein in preparation of drugs for cardiovascular disease. The invention employs RT-PCR method, extracts sequence 1 from total RNA of tentacles of sagartia rosea ( sequence 1 indicates) the protein coded by the gene( sequence 2 indicates) Recombinant actinocongestin protein in the invention has cardiac and anti-arrhythmia action, can prepare the cardiovascular disease drugs.

The invention relates to actinocongestin gene Sr4 and its coded protein, and also the uses of the said protein in preparation of drugs for cardiovascular disease. The invention employs RT-PCR method, extracts sequence 1 from total RNA of tentacles of sagartia rosea ( sequence 1 indicates) the protein coded by the gene( sequence 2 indicates) Recombinant actinocongestin protein in the invention has cardiac and anti-arrhythmia action, can prepare the cardiovascular disease drugs.