Sea anemone polypeptide toxin Hc-GQ as well as preparation method and application thereof

A technology of sea anemone peptides and toxins, which is applied in the field of peptides and can solve problems such as agricultural pollution, pesticide resistance of insect species, and excessive pesticide residues in agricultural products.

Active Publication Date: 2022-04-01
HAINAN MEDICAL COLLEGE
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Problems solved by technology

In recent years, with the abuse of chemical pesticides, many insect species have developed resistance and pesticide re

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  • Sea anemone polypeptide toxin Hc-GQ as well as preparation method and application thereof
  • Sea anemone polypeptide toxin Hc-GQ as well as preparation method and application thereof
  • Sea anemone polypeptide toxin Hc-GQ as well as preparation method and application thereof

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Embodiment Construction

[0034] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0035] The present invention proposes a purple spot sea anemone polypeptide toxin Hc-GQ, whose sequence is KCQCSACGAETSLCNFTSGACLCK, has 6 cysteines to form 3 disulfide bonds, and the disulfide bonds are C1-C6, C2-C5, C3-C4 .

[0036] The present invention also provides a method for preparing the polypeptide toxin Hc-GQ of Anemone anemone, said method comprising the following contents:

[0037] 1. Materials and methods

[0038] 1.1 Experimental materials

[0039] Chromatographic-grade trifluoroacetic acid (TFA) and chromatographic-grade acetonitrile ...

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Abstract

The invention discloses a sea anemone polypeptide toxin Hc-GQ as well as a preparation method and application thereof, linear peptide Hc-GQ is synthesized by adopting a polypeptide solid-phase synthesis method (SPPS), the linear peptide is subjected to HPLC purification, then the synthesized linear peptide is subjected to one-step oxidation folding by utilizing an air oxidation method, and the sea anemone polypeptide toxin Hc-GQ is obtained. Oxidized peptide Hc-GQ (C1-C6, C2-C5 and C3-C4) containing three disulfide bonds is obtained after directional oxidation of the disulfide bonds, and the final oxidized and folded product is subjected to HPLC (high performance liquid chromatography) purification and mass spectrum identification to obtain the sea anemone polypeptide toxin Hc-GQ, namely the oxidized peptide Hc-GQ. The insecticidal effect of the synthesized oxidized peptide Hc-GQ on tenebrio molitor is tested by an insect injection method, and a foundation can be laid for research and development of novel, efficient and safe biological insecticides.

Description

technical field [0001] The invention belongs to the technical field related to polypeptides, and relates to a purple spot sea anemone polypeptide toxin Hc-GQ and its preparation method and application. Background technique [0002] Arthropod pests are distributed in all corners of the world, destroy about 20-30% of the global food supply, and they are also the main culprit in the transmission of many human diseases. In recent years, with the abuse of chemical pesticides, many insect species have developed resistance and pesticide residues in agricultural products have exceeded the standard, and agricultural pollution has become increasingly serious. In order to solve the agricultural environmental crisis and ecological security problems, it is imminent to develop new, efficient and safe biopesticides. Nowadays, many countries in the world screen and identify various effective physiologically active ingredients from natural biological resources such as snake venom, scorpion ...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/08C07K1/06C07K1/04A01N37/46A01P7/04
CPCY02P20/55
Inventor 高炳淼廖燕玲符金星李明郭琦琦
Owner HAINAN MEDICAL COLLEGE
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