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Sea anemone polypeptide toxin Ap-GT, and preparation method and application thereof

A sea anemone polypeptide and toxin technology, which is applied in the preparation method of peptide, botanical equipment and method, application and other directions, can solve the problems of poor composition of ingredients, unfavorable to improve the efficacy and safety of medicine, and reduce the dosage of medicine. , Significant insecticidal effect, the effect of improving safety

Active Publication Date: 2021-12-03
HAINAN MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN111876457A discloses the preparation of mantle sea anemone enzymolyzed polypeptide and its insecticidal application. It is the extract of mantle sea anemone and is a mixture of polypeptides with various sequences. It is not easy to control the composition of the ingredients, which is not conducive to improving the efficacy and safety of the drug sex

Method used

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  • Sea anemone polypeptide toxin Ap-GT, and preparation method and application thereof
  • Sea anemone polypeptide toxin Ap-GT, and preparation method and application thereof
  • Sea anemone polypeptide toxin Ap-GT, and preparation method and application thereof

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Embodiment

[0038] 1. Materials and methods

[0039] 1.1 Experimental materials

[0040] Chromatographic-grade trifluoroacetic acid (TFA) and chromatographic-grade acetonitrile (Acetonitrile, ACN) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; water analytical C18 column (5 μm, 4.6mm×250mm) was purchased from Waters, USA; Elite preparative C18 column (10 μm, 10mm×250mm) was purchased from Dalian Elite Analytical Instrument Co., Ltd.; conventional molecular biology reagents such as plasmid extraction kits were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.

[0041] 1.2 Experimental Instruments

[0042] CEM automatic microwave peptide synthesizer (LibertyBlue, USA); reversed-phase high-performance liquid chromatography (ThermoFisher, Germany); triple quadrupole liquid chromatography-mass spectrometry (Shimadzu, Japan); desktop freeze-dryer (Saifei , China); microplate reader (MR-96A, Shenzhen Mindray); patch clamp system (Axon900A, USA).

[0043...

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Abstract

The invention relates to a sea anemone polypeptide toxin Ap-GT, and a preparation method and application thereof. The sea anemone polypeptide toxin Ap-GT is synthesized and has a more remarkable insecticidal effect for tenebrio molitor, and a half lethal dose is 14.8 nM. The sea anemone polypeptide toxin Ap-GT with a specific sequence is synthesized, the addition amount of the sea anemone polypeptide toxin Ap-GT can be effectively controlled in application, the effect of the sea anemone polypeptide toxin Ap-GT can be exerted to the maximum extent, the dosage of a medicament is reduced, and the safety is improved to the maximum extent. The marine polypeptide which has an efficient insecticidal effect and small toxicity to mammals is obtained, and a foundation can be laid for research and development of novel, efficient and safe biological insecticides.

Description

technical field [0001] The invention relates to the technical field of sea anemone, in particular to a sea anemone polypeptide toxin Ap-GT and its preparation method and application. Background technique [0002] Sea anemone (Sea Anemone), also known as sea chrysanthemum, belongs to the phylum Cnidaria, the class Anthozoa, and the subclass Coralia. Sea anemones are one of the most primitive metazoan groups and one of the oldest extant venomous marine animals. Molecular evidence and fossil data show that sea anemones originated 750 million years ago. There are many kinds of sea anemones. More than 1,100 species of sea anemones have been recorded in the world, belonging to 50 families and about 400 genera. The species of sea anemones in China account for about 1 / 10 of the world's total and are widely distributed in warm and tropical seas. The stinging cells on the tentacles of sea anemones can secrete venom to prey on fish, shellfish, copepods, crustaceans and worms. The veno...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/04C07K1/06A01N37/46A01P7/04
CPCC07K14/43595A01N37/46Y02P20/55
Inventor 高炳淼符金星廖燕玲吴鑫福
Owner HAINAN MEDICAL COLLEGE
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