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Method for industrially roughly purifying toxins of living body sea anemones

A sea anemone and toxin technology, applied in the field of marine biology, can solve the problems of low utilization rate of sea anemone resources, enzymatic damage and other problems, and achieve the effects of short regeneration time of toxins, less possibility of pollution, and less damage to sea anemones

Inactive Publication Date: 2017-10-17
熊廷珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the sea anemone toxin proteins obtained by the above two methods are destroyed by enzymatic hydrolysis of their own proteases, and sea anemone tentacles need to be collected. If industrial production is to be carried out, a large number of sea anemone tentacles are needed, which makes the utilization rate of sea anemone resources low. The present invention In order to solve the problems existing in the above two methods, a more optimized method for extracting crude poison from sea anemones is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Put 15g of cornstarch, 1g of xanthan gum, and 84g of purified water into a stainless steel pot, heat and boil, stir continuously until there is no precipitation, then pour it into a stainless steel square tank with a length of 20cm, a width of 12cm, and a depth of 2cm to cool and solidify. Cover the nylon gauze with a mesh diameter of 2mm that has been washed. When it is not completely solidified, cover it with a layer of nylon gauze. After solidification, carefully demould. Fix the net-covered starch solidified on stainless steel or plastic extension sticks, close to 20 living sea anemones in natural waters or artificially cultured, slightly agitate, induce sea anemones to defend against them, collect each one for 10 minutes, and recover the netting Rinse the starch solidified body with purified water, carefully tear off the nylon mesh, cut off the starch solidified body with a thickness of 5mm on both sides with a stainless steel knife, crush it completely with a stain...

Embodiment 2

[0019] Add 16g of potato starch, 2g of carrageenan, and 82g of purified water into a stainless steel pot and heat to boil. Stir continuously until there is no precipitation, then pour it into a 20cm long, 12cm wide, and 2cm deep stainless steel square tank to cool and solidify. The nylon gauze with a mesh diameter of 2mm has been washed. Before it is completely solidified, cover it with a layer of nylon gauze. After solidification, carefully remove the mold. Fix the net-covered starch solidified on stainless steel or plastic extension sticks, close to 26 living sea anemones in natural waters or artificially cultured, slightly agitate, and induce the sea anemones to defend against them. After collecting each one for 10 minutes, recycle the netting Rinse the starch solidified body with purified water, carefully tear off the nylon mesh, cut off the starch solidified body with a thickness of 5mm on both sides with a stainless steel knife, crush it completely with a stainless steel ...

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Abstract

The present invention proposes a method for the industrialization of crude and pure toxins in live sea anemones, which uses the methods of starch coagulation preparation, coating, toxin collection, enzymolysis, centrifugation, nickel column chromatography, and ultrafiltration to remove impurities to obtain sea anemone protein toxoids Crude pure liquid. Compared with the prior art, the beneficial effect of the present invention is: without using protease or proteolytic agent, there are many types of sea anemone protein toxoids obtained; only sea anemone toxic substances are collected without collecting sea anemone tentacles, and the Sea anemone has little damage, its regeneration time is short, and its production sustainability is extremely advantageous, which provides conditions for the future industrial use of sea anemone toxins; the collected sea anemone toxins are less likely to be polluted by external proteins, and the protein isolated from the preliminary purification is only Create a good foundation for further purification of sea anemone toxin protein.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for industrializing crude and pure toxins from living sea anemones. Background technique [0002] Sea anemones belong to the phylum Coelenterata, class Anthozoa, and subclass Coralia. There are many kinds of sea anemones. There are more than 1,000 species in the world, accounting for about 1 / 7 of the total number of coelenterates, and the species of sea anemones in China account for about 1 / 10 of the world. Sea anemones have a wide range of habitats and are distributed in various sea areas around the world. They are most abundant in tropical and subtropical sea areas, and are more widely distributed in the South my country Sea. The tentacles and body of sea anemones contain a large number of stinging cells. When they are mechanically or chemically stimulated by the outside world, the chemical substances in the stinging cells are released to capture ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/22C07K1/14
CPCC07K1/36C07K1/145C07K1/22C07K1/34
Inventor 潘爱芹
Owner 熊廷珍
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