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Method for preparing congestin through fermentation of marine bacteria

A technology for marine bacteria and sea anemones, which is applied in the field of using marine bacteria to ferment sea anemone toxins, can solve the problems of high cost and long cultivation period, and achieve the effects of low cost, short period and environmental friendliness.

Active Publication Date: 2018-12-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, sea anemone toxins can be prepared through large-scale cultivation of dinoflagellates and product extraction. This method not only has a long cultivation period and high cost, but also requires special algae cultivation equipment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Shake flask seed solution: take the marine bacterial strain (Nioella sp.) LZ7-4 preserved on a solid slope, inoculate it into a 1L Erlenmeyer flask filled with sterile culture solution, liquid volume: 200mL, 28°C Cultivate for 12 hours, take the culture for microscopic examination, and confirm that the bacteria grow well without any bacterial contamination, and then inoculate into the seed tank;

[0028] (2) Preparation of seed solution: Prepare 5L of culture solution according to the formula of the shake flask seed solution, add it to the seed tank, heat it with steam, sterilize at 121°C for 28 minutes, cool to 28°C, inoculate with the flame method, and inoculate the shake flask seed The solution was inoculated on the seed medium in the seed tank, and the composition of the seed medium was: peptone 5g, yeast extract 1g, potassium dihydrogen phosphate 0.05g and natural seawater 1000mL; the pH of the seed medium was 7.2; the culture conditions were: maintain The vent...

Embodiment 2

[0033] (1) Shake flask seed solution: take the marine bacterial strain (Nioella sp.) LZ7-4 preserved on a solid slope, inoculate it into a 1L Erlenmeyer flask filled with sterile culture solution, liquid volume: 200mL, 28°C Cultivate for 12 hours, take the culture for microscopic examination, and confirm that the bacteria grow well without any bacterial contamination, and then inoculate into the seed tank;

[0034] (2) Preparation of seed solution: Prepare 5L of culture solution according to the formula of the shake flask seed solution, add it to the seed tank, heat it with steam, sterilize at 121°C for 28 minutes, cool to 28°C, inoculate with the flame method, and inoculate the shake flask seed The solution was inoculated on the seed medium in the seed tank. The composition of the seed medium was: peptone 5g, yeast extract 1g, potassium dihydrogen phosphate 0.05g and natural seawater 1000mL; the pH of the seed medium was 7.2; the culture conditions were: maintain The ventilat...

Embodiment 3

[0039] (1) Shake flask seed solution: take the marine bacterial strain (Nioella sp.) LZ7-4 preserved on a solid slope, inoculate it into a 1L Erlenmeyer flask filled with sterile culture solution, liquid volume: 200mL, 28°C Cultivate for 12 hours, take the culture for microscopic examination, and confirm that the bacteria grow well without any bacterial contamination, and then inoculate into the seed tank;

[0040] (2) Preparation of seed solution: Prepare 5L of culture solution according to the formula of the shake flask seed solution, add it to the seed tank, heat it with steam, sterilize at 121°C for 28 minutes, cool to 28°C, inoculate with the flame method, and inoculate the shake flask seed The solution was inoculated on the seed medium in the seed tank, and the composition of the seed medium was: peptone 5g, yeast extract 1g, potassium dihydrogen phosphate 0.05g and natural seawater 1000mL; the pH of the seed medium was 7.2; the culture conditions were: maintain The vent...

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Abstract

The invention relates to the technical field of marine microorganisms and particularly relates to a method for preparing congestin through the fermentation of marine bacteria. The method comprises thefollowing steps: (1) preparing bottle-shaking seed liquid; (2) preparing seed liquid; (3) carrying out fermentation cultivation; (4) extracting and purifying fermentation liquid; and (5) purifying acrude extract: carrying out chromatography elution, collection and rotary evaporation on a prepared liquid phase, so as to obtain white powder, namely congestin. According to the method, congestin isproduced through the fermentation of a marine bacteria strain Nioella sp.LZ7-4, so that the fermentation time is short and is only 36-40 hours, and the energy consumption can be effectively saved; theyield is relatively high and can reach over 38ug / L; and the method is simple in steps, low in workload and relatively short in period, and adopted reagents and materials are common reagents for microbial fermentation tests, harmless to a human body and environment-friendly, so that the method has relatively high market prospects and economic values.

Description

technical field [0001] The invention relates to the technical field of marine microbial fermentation, in particular to a method for preparing sea anemone toxin by fermentation of marine bacteria. Background technique [0002] Palytoxins (PLTXs) are one of the most toxic non-protein phycotoxins known, and are listed as one of the largest non-polymeric natural products. The currently isolated and identified components of sea anemone toxins include ovatoxin (OVTX), mascarenotoxin (McTX) and ostreocin, etc. PLTXs can enter the human body through inhalation or through skin contact, causing symptoms such as dyspnea, fever, and eye discomfort. Sea anemone toxins seriously endanger the safety of marine ecosystems and human health, and have become a worldwide research hotspot. Sea anemone toxin is a neurotoxin, which has important applications in the detection of harmful red tides, neurophysiology, medical diagnosis, drug development, and biochemical warfare agents. In terms of me...

Claims

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Application Information

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IPC IPC(8): C12P19/44C07H7/06C07H1/06C12R1/01
CPCC07H1/06C07H7/06C12P19/44
Inventor 杨桥张晓玲穆军蒋志伟张若男
Owner ZHEJIANG OCEAN UNIV
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