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Black skin termitomyces albuminosus mycelium culture method

A culturing method and mycelium technology are applied in the field of cultivating the mycelium of A. black-skinned fungus, which can solve the problems that artificial cultivation is difficult to overcome, and no artificial batch cultivation is successful, and achieves high organic germanium content and high-efficiency medical and health care effects. , the effect of short fermentation cycle

Inactive Publication Date: 2015-09-09
FUJIAN WANYI DIANZHONGDIAN E COMMERCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its artificial cultivation is difficult to conquer, so far there is no precedent for successful artificial batch cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Described method comprises as follows:

[0018] 1. After activation, select the mother species of Gallinarum nigritiformis and inoculate it on the slant medium, and expand the culture until the mycelium covers more than 2 / 3 of the slant;

[0019] 2. Select a strain with good mycelium growth and inoculate it into the liquid medium, and place it in a shaker at 24°C for constant temperature cultivation for 4 days;

[0020] 3. Put the bacteria cultivated in step (2) into a fermenter for cultivation. The fermentation temperature is 24°C, the rotation speed is 125rpm / min, and the cultivation time is 96h. Dry in an oven to constant weight.

[0021] The composition of the slant medium is: 200g of potatoes, 25g of glucose, 2.5g / L of yeast extract, 3.5mg / L of VB1, 1.5g / L of peptone, 1L of water, and a pH of 6.5.

[0022] The composition of the liquid medium is: corn flour 25g / L, bran 15g, Vc 2mg / L, VB1 3.5mg / L, yam extract 4g / L, water 1L, pH 6.5, and the liquid culture After t...

Embodiment 2

[0025] Described method comprises as follows:

[0026] 1. After activation, select the mother species of Gallinarum nigritiformis and inoculate it on the slant medium, and expand the culture until the mycelium covers more than 2 / 3 of the slant;

[0027] 2. Select a strain with good mycelium growth and inoculate it into the liquid medium, and place it in a shaker at 24°C for constant temperature cultivation for 3 days;

[0028] 3. Put the bacteria cultivated in step (2) into a fermenter for cultivation. The fermentation temperature is 24°C, the rotation speed is 120rpm / min, and the cultivation time is 72h. Dry in an oven to constant weight.

[0029] The composition of the slant medium is: 200 g of potatoes, 30 g of glucose, 3.5 g / L of yeast extract, 14 mg / L of VB, 2 g / L of peptone, 1 L of water, and a pH of 7.0.

[0030] The composition of the liquid medium is: corn flour 30g / L, bran 20g, Vc 3.5mg / L, VB1 4mg / L, yam extract 6g / L, water 1L, pH 7.0, and the prepared liquid cultu...

Embodiment 3

[0033] 1. After activation, select the mother species of Gallinarum nigritiformis and inoculate it on the slant medium, and expand the culture until the mycelium covers more than 2 / 3 of the slant;

[0034] 2. Select the strains with good mycelium growth and inoculate them into the liquid medium, and place them in a shaker at 23°C for constant temperature cultivation for 5 days;

[0035] 3. Put the bacteria cultivated in step (2) into a fermenter for cultivation. The fermentation temperature is 25°C, the rotation speed is 125rpm / min, and the cultivation time is 96h. Dry in an oven to constant weight.

[0036] The composition of the slant medium is: 200g of potatoes, 27g of glucose, 3g / L of yeast extract, 4mg / L of VB1, 1.7g / L of peptone, 1L of water, and a pH of 7.5.

[0037] The composition of the liquid culture medium is: corn flour 27g / L, bran 18g, Vc 3mg / L, VB1 3.7mg / L, yam extract 6g / L, water 1L, pH 7.5, and the prepared liquid culture After the base was sterilized at hig...

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Abstract

The invention provides a black skin termitomyces albuminosus mycelium culture method which includes the steps: selecting and activating black skin termitomyces albuminosus mother strains, inoculating the strains on an inclined medium and enlarging culture until mycelia overgrow an inclined surface by more than 2 / 3; selecting strains with fine mycelium growth, inoculating the strains in a liquid medium and placing the liquid medium in a swing bed with the temperature of 23-24 DEG C to perform constant temperature culture for 3-5 days; throwing the strains cultured in the second step into a fermentation tank for culture to obtain mycelia by filtering, and drying the mycelia in a drying oven with the temperature of 65 DEG C to reach constant weight. The fermentation temperature is 22-25 DEG C, the rotating speed is 120-125rpm / min, and culture time is 72-96h. Fermentation cycle is short, mycelium yield is high, pollutants, waste and the like in the production process are avoided, organic germanium content is high, and germanium can be supplemented.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for cultivating the mycelia of Gallia nigris. Background technique [0002] Gallium fir fungus has high edible and medicinal value, and it is also one of the traditional medicinal fungi in my country. It is favored by people because of its tender and mellow aroma, tender meat, white as jade, unique taste, edible both raw and cooked, dual-purpose food and medicine, and rich nutrition. Gallinarum has the effects of "benefiting the stomach, clearing the mind, treating hemorrhoids and lowering blood fat", nourishing blood and moistening dryness, strengthening the spleen and stomach, etc., and can be used to treat loss of appetite, chronic diarrhea, and blood from hemorrhoids. Now medical research has found that the ergosterol substance and the effective ingredients for treating diabetes are contained in the gallium fir, which has obvious effect on loweri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05G3/00
Inventor 张可池
Owner FUJIAN WANYI DIANZHONGDIAN E COMMERCE CO LTD
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