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Bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof

A technology of Bacillus amylogenus and ZFH-3, which is applied in the fields of disease control and plant protection, and can solve the problems of products not involved in the processing of microbial agents and low bacterial content

Active Publication Date: 2015-05-20
GUANGDONG GEOLONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no biological product of Bacillus amyloliquefaciens for soil-borne diseases, only the patent of Jiangsu Academy of Agricultural Sciences "Bacillus amyloliquefaciens strain B-1619 and its application in the prevention and treatment of soil-borne diseases of Solanaceae vegetables", but its disadvantage is that : The bacteria powder produced by fermentation is measured by the amount of bacteria, and the amount of bacteria is relatively low. It does not involve the processing of microbial inoculum products, and it is still a long way from practical application.

Method used

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  • Bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof
  • Bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof
  • Bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0067] 1. Preparation and identification of Bacillus amyloliquefaciens subsp. plantarum ZFH-3

[0068] 1. Sample collection

[0069] Collect soil samples from old forests in Datian County, Sanming City, Fujian Province, collect edible fungi of the Basidiomycetes Agaricaceae Russulaceae, and dry them naturally; cut off the roots of edible fungi, grind them into powder, and mix them evenly to obtain root samples of edible fungi; soil samples Grind into powder, mix evenly, and obtain soil samples;

[0070] 2. Isolation of strains and screening and purification of resistant bacteria

[0071] (1) Sample dilution: From the 2 samples (fungus root sample and soil sample) that were mixed uniformly, each weighed 5g and dissolved it in a conical flask filled with 45ml sterile water, and vibrated with a 150rp oscillator to form a uniform 10 -1 diluent, and then use a 1ml pipette gun to draw 1ml of the diluent into a test tube filled with 9ml sterile water, and shake it well to make 10 ...

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Abstract

The invention relates to a bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof and belongs to the field of plant protection and disease prevention and treatment. The microbial agent per g contains 2*10<10>-1.2*10<11>cuf bacillus amyloliquefaciens plant subspecies ZFH-3 living spores, and the ZFH-3 strain is classified and named Bacillus amyloliquefaciens subsp.Plantarum, wherein the collection number is CGMCC No. 9884. The agent has a remarkable inhibiting effect on various pathogens of crops, shows a relatively good preventing and treating effect on prevention and treatment of soil-borne diseases and can effectively prevent and treat soil-borne diseases of vegetables, so that the loss is damaged and the field efficacy is over 59%. The microbial agent is non-toxic and safe to human bodies, replaces chemical agents for preventing and treating soil-borne diseases, conditioning soil and promoting plant growth, and has a good application prospect.

Description

technical field [0001] The invention belongs to the fields of plant protection and disease prevention and control, and mainly relates to a microbial bacterial agent of bacillus amyloliquefaciens plant subspecies ZFH-3 and its application, especially the application in the prevention and control of soil-borne diseases. Background technique [0002] Soil-borne diseases are a general term for plant diseases that are transmitted through the soil. They refer to diseases caused by pathogens living in the soil, such as fungi, bacteria, nematodes and viruses, that attack crops from the roots or stem bases of crops when conditions are suitable. There are many types of soil-borne diseases in China. Common soil-borne diseases include damping-off, blight, fusarium wilt, verticillium wilt, soft rot, blight, root rot, clubroot, bacterial wilt, etc. The general symptoms of plants infected by pathogens are blight type, damping type, blight type, wilting type and verticillium wilting type. T...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N25/14A01N25/04A01P3/00A01P1/00C12R1/07
CPCA01N25/04A01N25/14A01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 吴日章
Owner GUANGDONG GEOLONG BIOTECH
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