Geobacterium galactose BWTGW1.1 and application thereof

A galactose and geological technology, applied in the field of environmental microorganisms, can solve the problems of harmlessness and low fertility, long time-consuming natural fermentation, serious odor pollution, etc., achieve great application potential, promote the fermentation process and the duration of the high-temperature stage , the effect of strong growth adaptability

Pending Publication Date: 2021-11-30
广东博沃特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional composting method mainly uses the fermentation of indigenous microorganisms in organic waste, which has many disadvantages such as long time-consuming natural fermentation, serious odor pollution, harmlessness and low fertility.

Method used

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  • Geobacterium galactose BWTGW1.1 and application thereof
  • Geobacterium galactose BWTGW1.1 and application thereof
  • Geobacterium galactose BWTGW1.1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation, purification and preservation of Geobacter galactosus BWTGW1.1

[0030] In March 2020, three mid-layer samples were collected from Dongguan City Center Designated Slaughterhouse Co., Ltd. during the high-temperature stage (50°C) of composting and fermentation of pig manure and sludge organic waste.

[0031] Isolation, purification and preservation of high-temperature bacteria: Weigh 10 g of the collected three samples and suspend them in 50 mL of PBS (pH 7.4, concentration 0.2 mol / L), shake vigorously for 5 min, centrifuge at 1000 r / min for 3 times, each time for 5 min, and collect the supernatant to the centrifuge tube. Dilute the supernatant to different concentrations, take 10 -3 、10 -4 、10 -5 Gradient bacterial solution was spread on LB agar plate and incubated at 60°C for 48 hours. Pick the strains with better growth and larger colonies, continue to streak and separate on the LB agar plate, and purify repeatedly.

[0032] The purified colo...

Embodiment 2

[0033] Example 2 Identification of 16S rDNA of Geobacter galactosus BWTGW1.1

[0034] (1) Morphological identification: Spread the screened bacterial strain BWTGW1.1 on an LB plate, culture at 60°C for 24 hours, and observe its colony characteristics; take a single colony smear, Gram staining, and observe its cell morphology under a microscope , see the result figure 1 .

[0035] (2) Molecular identification of 16S rRNA gene:

[0036]Genomic DNA of strain BWTGW1.1 was extracted, and the PCR product was amplified with bacterial 16S rDNA gene amplification general primer 27F / 1492R (5'-AGAGTTTGATCCTGGCTCAG-3' and 5'-TACGACTTAACCCCAATCGC-3') and sent to Shanghai Meiji Biomedical Technology Co., Ltd. (Guangzhou Branch) performed sequence sequencing, and the length of the 16S rRNA sequence obtained after sequencing was 1447bp, and the sequence is shown in SEQ ID NO.1. The sequencing results were compared with the 16SrDNA sequences in the NCBI and EzBioCloud databases for homology...

Embodiment 3

[0039] Example 3 Evaluation of the ability to produce thermostable protease of Geobacter galactosus BWTGW1.1

[0040] The ability to produce high-temperature-resistant protease was evaluated by the plate hydrolysis circle detection method:

[0041] The main media and reagents used are as follows:

[0042] Protein culture medium: 40.0g skimmed milk powder, 10.0g soluble starch, 3.0g yeast extract, 2.0g potato flour, 15.0g agar, add distilled water to 1000mL, pH 7.0-7.2. Sterilize at 121℃ for 20min at high temperature and high pressure, pour plate .

[0043] Spot the activated Geobacter galactosus BWTGW1.1 on the protein medium plate respectively, place them in a 60°C incubator and cultivate them for 48 hours, and observe whether there is a hydrolyzed transparent circle around the colonies, which can be directly reflected qualitatively. Whether the strain has the ability to secrete and produce thermostable protease.

[0044] Observation obtained by the above-mentioned method ...

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Abstract

The invention discloses a geobacterium galactose BWTGW1.1 and an application thereof. Geobacillus galactosidassius BWTGW1.1 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on November 11, 2020, the address of the GDMCC is the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC No. 61374. The Geobacterium galactosidasius BWTGW1.1 disclosed by the invention is strong in growth adaptability in high-temperature compost of pig manure sludge wastes, is active in a high-temperature stage, can generate high-temperature-resistant protease, and has a relatively great application potential in aerobic compost of livestock and poultry breeding organic wastes.

Description

Technical field: [0001] The invention relates to the technical field of environmental microorganisms, in particular to a Geobacter galactosus BWTGW1.1 and its application in the treatment of solid organic waste from livestock and poultry breeding. Background technique: [0002] In recent years, domestic and foreign experience in the treatment of aquaculture organic waste has shown that high-temperature aerobic composting of aquaculture waste to produce high-quality organic fertilizer is an important way to recycle this type of waste. In the process of aerobic composting, on the one hand, the biodegradable high-molecular organic matter is decomposed by the rapid reproduction of microorganisms, and a new stable high-molecular organic matter --- humus (an important active factor that constitutes soil fertility) is synthesized; on the other hand The massive reproduction of microorganisms releases heat, which makes the heap continue to heat up in a short period of time, thereby k...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/52C05F3/00C05F17/20C12R1/01
CPCC12N9/52C05F3/00C05F17/20Y02W30/40Y02A40/20
Inventor 陈猛谢小林周莲李成江刘玉敏朱红惠陈美标
Owner 广东博沃特生物技术有限公司
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