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Preparation method for sea anemone polysaccharide and application thereof to tumor resistance

A sea anemone polysaccharide and sea anemone technology, applied in the field of preparation of sea anemone polysaccharides with anti-tumor activity, can solve the problems of unreported research on the biological activity of A. good solubility effect

Active Publication Date: 2014-12-24
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The coast of Zhejiang Province is rich in A. elegans sea anemone, but there is no report on the extraction method and biological activity of the polysaccharides of A.

Method used

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  • Preparation method for sea anemone polysaccharide and application thereof to tumor resistance
  • Preparation method for sea anemone polysaccharide and application thereof to tumor resistance
  • Preparation method for sea anemone polysaccharide and application thereof to tumor resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0041] b) cutting the homogenate of crushed sea anemone for 10 minutes, then adding water with a volume 4 times the volume of the homogenate to the homogenate, and leaching at 30° C. for 2 hours to obtain an extract;

[0042] c) centrifugation of the extract: 8000rpm, 4°C, centrifugation for 5min, to obtain a supernatant;

[0043] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0044]e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 4 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it st...

Embodiment 2

[0049] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0050] b) Cut the homogenate of crushed sea anemone for 15 minutes, add 6 times the volume of water to the homogenate, and extract at 50° C. for 3 hours to obtain an extract;

[0051] c) centrifugation of the extract: 9000rpm, 4°C, centrifugation for 10min to obtain a supernatant;

[0052] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0053] e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 6 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it stand for the same time, centrifuge and sep...

Embodiment 3

[0058] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0059] b) Cut the homogenate of crushed sea anemone for 20 minutes, add 10 times the volume of water to the homogenate, and extract at 70° C. for 4 hours to obtain an extract;

[0060] c) centrifugation of the extract: 10000rpm, 4°C, centrifugation for 15min to obtain a supernatant;

[0061] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0062] e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 8 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it stand for the same time, centrifuge and s...

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Abstract

The invention discloses a preparation method for sea anemone polysaccharide. The preparation method comprises the following steps: crushing raw materials by adopting a constant-temperature high-speed shearing method; performing water extraction on the crushed raw materials; performing three-level alcohol precipitation of 30 percent, 60 percent and 80 percent; then performing enzymolysis by using papain and deproteinization by using sevag to obtain three kinds of sea anemone polysaccharide containing higher saccharide content. The three kinds of sea anemone polysaccharide prepared through the preparation method disclosed by the invention can be used for preparing tumor-resisting medicines and particularly can be used for preparing medicines for resisting lung cancer, prostatic cancer or ascitic tumor.

Description

1. Technical field [0001] The invention relates to the field of deep processing of marine products, in particular to a preparation method of sea anemone polysaccharide with anti-tumor activity. 2. Background technology [0002] The environment of high salinity, high pressure, low temperature, hypoxia, oligonutrition and insufficient light in the ocean has contributed to the richness and diversity of the structure and function of marine polysaccharides. Numerous studies have shown that marine animal polysaccharides have anti-tumor, anti-virus, anti-cardiac Vascular diseases, anti-oxidation and immune regulation and other biological activities, such as: abalone polysaccharides (Yin Hongling, Yang Jingfeng. Research on Enzymatic Extraction of Abalone Polysaccharides, Food and Fermentation Industry, 2006, 32(12): 158), sea cucumber polysaccharides (Cai Binxin , Wu Chengye. Separation and purification of polysaccharides from sea cucumbers and their main biological activities, Fuj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 何荣军赵月钧王茜孙培龙
Owner ZHEJIANG UNIV OF TECH
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