Application of molecule miR-150 in screening preparation of colorectal cancer detecting medicament

Abstract

The invention provides application of molecule miR-150 in screening preparation of a colorectal cancer detecting medicament. The molecule miR-150 is screened out by using a muParaflo microfluidic chip by the inventor of the invention; a paraffin section preparation of a clinical colorectal cancer patient is detected by an established digoxigenin labeled probe of miR-150 and an established detection method of qRT-PCR (Quantitative Real-Time-Polymerase Chain Reaction), respectively; results show that miR-150 low expression level in the clinical colorectal cancer patient indicates short lifetime of the patient and poor sensitivity of the patient to postoperative chemotherapy, and miR-150 high expression level indicates long lifetime of the patient and good sensitivity of the patient to postoperative chemotherapy. Compounds having high sensitivity to miR-150 can be screened by the method, and the compounds can be used for developing a kit for detecting the colorectal cancer. The kit is composed of compounds sensitive to the molecule miR-150 as active ingredients and a medicinal carrier. Simple detection of the miR-150 expression level is advantageous for monitoring of the colorectal cancer patient after healing and determination of chemotherapy sensitivity of the patient, and thus provides an effective method for clinical prevention and treatment of the colorectal cancer; high clinical application value and social benefit are created.

Description

technical field [0001] The invention relates to medicinal chemistry, in particular to the application of biomarker molecule miR-150 in screening, preparing and detecting colorectal cancer drugs. Background technique [0002] Colorectal cancer is one of the common malignant tumors, and its incidence rate ranks third among malignant tumors in the world. With the continuous improvement of living standards and changes in diet structure, the incidence of colorectal cancer in China is also increasing year by year, especially in big cities The growth rate is even faster. For example, during the 20 years from 1984 to 2004, the incidence of colorectal cancer in Shanghai increased from 16 / 100,000 to 23.61 / 100,000, and from 14.26 / 100,000 to 20.43 / 100,000 for women. At present, there are many factors that affect the curative effect of colorectal cancer, and the lack of effective indicators for prognosis and treatment sensitivity monitoring is still an important problem that needs to be ...

AI Technical Summary

Problems solved by technology

However, these existing studies only screen miRNAs related to colorectal cancer at the basic level, and use in vitro cell experiments and in vivo animal experiments to confirm their potential biological functions and molecular mechanisms of action. From the perspective of samples, screening and analysis of colorectal cancer biomarkers with potential clinical prognosis and treatment monitoring value failed to identify effective key miRNA molecules

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