34 results about "Bacillus psychrosaccharolyticus" patented technology

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion / g to 1 billion / g and an organic matter content of 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

The invention relates to a Bacillus saccharophila used for degrading alginic acid, and an application method thereof. The application method comprises the following steps: 1, milling dried alga to form powder, adding water to the alga powder according to a certain ratio, filling a fermentation tank with the obtained solution, and sterilizing the solution at a certain temperature for a period of time; 2, preparing a seed liquid: screening a fermentation strain YIC~ALG3 capable of degrading alginic acid, inoculating the fermentation strain YIC~ALG3 to a 2216E liquid medium, and culturing the fermentation strain YIC~ALG3 overnight; and 3, adding the obtained seed liquid to the fermentation tank according to a certain mass ratio, fermenting the seed liquid under certain conditions, and processing a brown alga raw material through using microbial fermentation to prepare an alga liquid. The fermentation process is stable and controllable, the fermentation period is short, and the alga liquid has substantial efficacy, and is suitable for large-scale production.

The invention provides a microbial agent for preventing and treating cumquat huanglongbing and a preparation method thereof, and particularly provides a microbial agent for preventing huanglongbing of cumquat, citrus shantianju and citrus and a preparation method thereof. The microbial agent is formed by fermenting and then mixing bacteria like Gulake streptomyces, Kawuer streptomyces, bacillus psychrosaccharolyticus, nissl bacillus and tufted trichoderma proportionally. Procreation and propagation of gram negative bacteria are inhibited by means of root irrigation and foliage spray, bacteria are used to treat bacteria, and harmful pathogenic bacteria in soil are killed, so that tree root systems are more developed, tree cell tubes are smooth, yellow leaves of fruit trees are turned green, and healthy new treetops are enabled to grow; the microbial agent generates insecticidal antibiotics like flavensomycin and inhibits or damages development process of eubactericera and eggs of other disease-transmitting insect (like citrus aphid), so that adult rate is reduced, orchard pest occurrence quantity is reduced, transmission media of huanglongbing are cut off, and effects on preventing and controlling citrus huanglongbing are realized.

The invention belongs to the technical field of biology and particularly relates to a production process of bacillus psychrosaccharolyticus extracellular polysaccharide and an application thereof to fertilizers. The bacillus psychrosaccharolyticus extracellular polysaccharide has good water-retaining property on leaves, stems and peripheral soil of plants, can adsorb heavy metal ions to reduce the environment pollution and can also inhibit various fungus diseases, the extracellular polysaccharide can be widely applied to the fertilizers of leaf surface fertilizers, drop irrigation fertilizers, bacterial fertilizers and the like through being used as a novel functional fertilizer, and the application prospects are wide. The production process of the bacillus psychrosaccharolyticus extracellular polysaccharide comprises the following steps that bacillus psychrosaccharolyticus HD-3 strains are obtained through separation, the seed culture liquid is prepared, and polysaccharide products are obtained through fermentation, extraction and drying. Because the culture medium recipe and the production process are optimized, the extracellular polysaccharide has high yield and low cost, and the finished products contain more than 90 percent of polysaccharide.

The invention provides an organic fertilizer for improving saline soil and a preparation method thereof. The organic fertilizer is prepared through a technology of combining three-step fermentation and a compound pelletizing method by taking wheat straw as the main raw material and being assisted by effective active strains such as bacillus psychrosaccharolyticus, EM bacteria, nitrifying bacteria and bacillus megaterium; the organic fertilizer has the effects of organic fertilizers and bio-organic fertilizers, is rich in natural organic nutrients and contains a large number of probiotics, soil hardening can be broken, the balance of microorganisms in soil is effectively adjusted, the phenomena of soil salinization caused by relying on fertilizer abuse all year round and the like can be eliminated, water and fertilizer penetration of the oil is enhanced, stress resistance of crops is improved, the yield of the crops is increased, and the quality of agricultural products is improved; releasing of organic matter in the soil can be promoted by the organic fertilizer, the physical and chemical structure of the saline soil is improved, agglomerates and permeability are improved, the soil fertility is improved, and the organic fertilizer is a good plant area soil modifier.

The invention discloses a method for ensiling enteromorpha and a method for preparing enteromorpha crude polysaccharide. According to the method for ensiling the enteromorpha and the method for preparing the enteromorpha crude polysaccharide, a compound microbial agent which is a mixture of lactobacillus plantarum, lactobacillus brevis, bacillus siamensis and bacillus psychrosaccharolyticus is used for carrying out enteromorpha ensiling processing; after the enteromorpha is processed through the method for ensiling the enteromorpha, a solid cellulose structure can be easily resolved to be utilized; due to the fact that the acid hydrolysis technology is adopted, consumption of acid can be reduced, the degradation efficiency is also improved, the content of solid content in hydrolysate is increased, concentration does not need to be carried out, spray drying can be directly carried out, and production cost is reduced. The prepared enteromorpha crude polysaccharide can improve immunity and stress resistance capacity of crops. The method for ensiling the enteromorpha and the method for preparing the enteromorpha crude polysaccharide effectively solve the technical problems of enteromorpha ensiling processing, achieve long-term storage of the fresh enteromorpha, and are simple in operation, small in number of technological processes, low in production cost and applicable to industrialized mass production.

The invention discloses a microbial agent for preventing and controlling lepidopteran pests. A raw material for preparing the microbial agent is prepared from the following components in parts by weight: 20 to 30 parts of fermented liquid of bacillus thuringlensis, 10 to 15 parts of fermented liquid of brevibacillus laterosporus, 5 to 10 parts of fermented liquid of bacillus psychrosaccharolyticus, 5 to 10 parts of fermented liquid of bacillus cereus and 5 to 10 parts of fermented liquid of yeasts. In addition, the invention further provides a preparation method for the microbial agent for preventing and controlling the lepidopteran pests. According to the microbial agent and the preparation method, five microbial agents are matched for use for the characteristics of the lepidopteran pests and each microbial agent, wherein the bacillus thuringlensis is a main insecticide, the brevibacillus laterosporus, the bacillus psychrosaccharolyticus and the bacillus cereus have synergistic effects on the bacillus thuringlensis, and the yeasts have protection and repair effects on leaves damaged by the lepidopteran pests. In addition, the microbial agent is free of residuals after use, free of drug resistance and environment-friendly.

The invention discloses a plant resistance inducer containing neoagaro-oligosaccharides, and belongs to the technical field of plant nutrition. The plant resistance inducer comprises the following rawmaterials: neoagaro-oligosaccharides, Bacillus psychrosaccharolyticus and tamarindus polysaccharides, wherein the mass ratio of neoagaro-oligosaccharides to tamarindus polysaccharides is (20 to 40):(5 to 15). According to the plant resistance inducer, neoagaro-oligosaccharides and tamarindus polysaccharides are taken as main components, an efficient plant resistance inducer is prepared through the supplementation with Bacillus psychrosaccharolyticus, the effect of the product is promoted obviously through a synergistic interaction effect among the raw materials, the crop synergistic interaction of crops under the environmental stress conditions of low temperature, drought, salt and alkali and the like can be promoted obviously, and the production increasing capacity under the environmental stress conditions is promoted. The stress resistance effect of the product under the environmental stress conditions of salt and alkali, drought, low temperature and the like is obvious, and the inducer has extremely remarkable effects for field crops such as wheat and rice as well as fruits and vegetables such as cucumber and tomato.

The invention discloses a microbial bacterial agent and a preparation method thereof, and solve the technical problems that chemical pesticides are not safe on people and livestock, so that the environment pollution can be easily caused; the growth promotion and prevention effects are relatively low. The microbial bacterial agent and the preparation method belong to the technical field of microbial preparations. The microbial bacterial agent is prepared from microbial ingredients of bacillus thuringiensis fermentation bacterial liquid, brevibacillus laterosporus fermentation bacterial liquid,bacillus psychrosaccharolyticus fermentation bacterial liquid, bacillus cereus fermentation bacterial liquid, yeast fermentation bacterial liquid, bacillus subtilis fermentation bacterial liquid andbacillus megatherium fermentation bacterial liquid, and natural extract ingredients of bead tree seeds, fruit peel, radix sophorae flavescentis and hellebore grass. The preparation method has the advantages that the plant source pesticides and the microbial bacterial agent are effectively combined to be used as fertilization or leaf fertilizers to be used in vegetable planting; the prevention effect is good; the growth promotion is obvious; safety and environment protection are realized.

The invention discloses an extraction method of shark chondroitin sulfate, and belongs to the technical field of marine organisms. The shark chondroitin sulfate with the high oxidation resistance is researched and developed by taking shark cartilage powder as a raw material and integrating a biological enzymolysis technology, a mixed microbial fermentation technology and a modern biological separation technology, wherein a compound enzyme system adopted in the biological enzymolysis technology is one or a mixture of more of papain, trypsin, neutral protease, pepsin and flavourzyme, and fermentation strains for mixed microbial fermentation mainly include aspergillus oryzae, bacillus psychrosaccharolyticus and bacillus subtilis. The shark chondroitin sulfate prepared by the process is high in yield, has the relatively high oxidation resistance and the remarkable anti-tumor effect, and can be widely applied to medical health-care foods for preventing tumors and the like.

The invention relates to a production method of a liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method comprises: grinding dried seaweed into powder, adding the seaweed powder into water according to a certain ratio, putting the obtained product in a fermentation tank, and performing sterilization at a certain temperature for certain time; preparing a seed solution, which includes performing screening to obtain YIC-ALG3 saccharophilia bacillus as a fermentation strain for degrading alginic acid, inoculating a liquid medium with the YIC-ALG3 saccharophilia bacillus and performing cultivation for certain time; adding the seed solution into the fermentation tank according to a certain mass ratio; performing fermentation at a certain condition; adjusting the fermentation condition after the fermentation and performing preservation for certain time; adjusting the fermentation condition again and performing replenishment with the seed solution; performing filter pressing after the adjustment is carried out for three times to obtain a saccharophilia bacillus filtrate; and adding water, the saccharophilia bacillus filtrate and potassium glutamate in a stirring tank, and performing stirring till the mixture is completely dissolved to obtain the liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method is stable and controllable in fermentation process, short in fermentation period, and obvious in product effect, and is suitable for large-scale production.

The invention relates to a method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid. Dried seaweed is ground into powder, the seaweed powder is added to water according to a certain proportion, and the mixture is placed in a fermentation tank and then sterilized at a certain temperature for a certain period of time; a seed solution is prepared; a fermentation strain YIC-ALG3 glycotropic bacillus capable of degrading alginic acid is obtained through screening, and a liquid culture medium is then inoculated with the fermentation strain for culture lasting a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio to be fermented under a certain condition, the fermentation condition is adjusted after fermentation, the fermentation condition is adjusted again and the seed solution is supplemented after a certain period time of storage, glycotropic bacillus is obtained after three times of adjustment, and glycotropic bacillus and organic matter containing amino acid are evenly stirred to obtain the water-soluble fertilizer containing glycotropic bacillus and amino acid. The fermentation process is stable and controllable, fermentation period is short, product functions are remarkable, and the fertilizer is suitable for large-scale production.

The invention discloses a functional biological fertilizer which is prepared from 6 parts of isothiocyanate, 8 parts of ammonium molybdate, 11 parts of salicylic acid, 4 parts of zinc sulfate, 10 parts of silicon oxide, 4 parts of boric acid, 1 part of bacillus psychrosaccharolyticus, 4 parts of monopotassium phosphate, 5 parts of ethyl valerate, 4 parts of copper sulfate, 2 parts of manganese sulfate, 1 part of bacillus megatherium, 1 part of magnesium sulfate and 1 part of nitrobacteria. The functional biological fertilizer has the benefits that the fertilizer efficiency can be improved, insects can be prevented and treated, and the survival rate of microorganisms in the functional biological fertilizer can be also improved.

The invention discloses a preparation method of a biological organic fertilizer for loosening soil. The preparation method has the benefits that firstly, cheap organic materials are taken as main raw materials, and a microbial strain fermentation process is adopted for preparation, and a product has the characteristics of an organic fertilizer and the biological organic fertilizer and has better soil loosening and improving effects; secondly, bacillus psychrosaccharolyticus, nitrobacteria, EM bacteria and bacillus megatherium and other microorganism rotten strains are integrated step by step through a 'three-step fermentation method', so that the strain amplification and antagonism effects caused by simultaneous fermentation of multiple strains are avoided, the effective activities of the four strains are greatly retained, and the technical effects of the invention are obviously improved; thirdly, the production cost is low, the industrial production and the large-scale application are facilitated, and the defect of high cost existing in farmland soil compaction improved by the organic fertilizer and a soil conditioning agent product which are being sold in the current market is overcome.

The invention discloses a method for improving antioxidant ability of a trepang saponin extracting solution, and belongs to the technical field of marine organisms. Waste trepang internal organs obtained by deep processing of trepang are used as raw materials, and a modern bioseparation technique, a biological enzymolysis technique, a mixed bacterium microorganism fermentation technique and an ethanol precipitation and resin adsorption technique are integrated, so that the trepang saponin extracting solution high in in-vitro antioxidant ability and nutrient health-care efficacy is developed, wherein a compound enzyme preparation compound enzyme preparation is a mixture of one or more of proteases, non-starch polyase and pectinase, and mixed bacterium aerobic zymocyte mainly comprises candida utilis, bacillus licheniformis and bacillus psychrosaccharolyticus. The obtained trepang saponin has high antioxidant ability and high light spectrum properties, and can be efficiently utilized inthe fields of health care, foods, medicines and the like. The extraction method is high in extraction rate of the trepang saponin, can realize resource reusing of wastes obtained by processing of thetrepang, and is convenient for industrial production and broad in market prospects.

The invention relates to a production method of a water soluble fertilizer containing saccharophilous bacillus and a humic acid, wherein dried seaweed is grinded into powder, the seaweed powder is added into water at certain proportion, and is sterilized at certain temperature for certain time after packaged into a fermentation tank; a seed solution is prepared by screening to obtain a fermentation strain, saccharophilous bacillus YIC-ALG3, capable of degrading alginic acid, inoculating to a liquid medium and culturing for a period of time, adding the seed solution to the fermentation tank according to mass ratio, fermenting at certain conditions, adjusting the fermentation condition after fermentation, adjusting the fermentation condition again and supplementing the seed solution after storing for a period of time, and adjusting for three times to obtain the saccharophilous bacillus; the saccharophilous bacillus, a nitrogenous fertilizer, a phosphatic fertilizer, a potassic fertilizer and an organic matter containing the humid acid are evenly stirred to obtain the water soluble fertilizer containing the saccharophilous bacillus and the humic acid. The fermentation process is stable and controllable, the fermentation period is short, the effects of the product are remarkable, and the production method is suitable for scale production.

The invention relates to a Bacillus saccharophilus for degrading alginic acid and an application method thereof. The dried seaweed is ground into powder, and the seaweed powder is added into water according to a certain ratio, and after being put into a fermenter, at a certain temperature, a certain Sterilization under time; preparation of seed liquid: a fermentation strain YIC~ALG3 capable of degrading alginic acid was screened and inoculated into 2216E liquid medium for overnight cultivation; the seed liquid was added to the fermenter according to a certain mass ratio, and under certain conditions Fermentation, after fermentation, the raw material of brown algae is processed by microbial fermentation to prepare seaweed liquid. The fermentation process of the invention is stable and controllable, the fermentation period is short, the product has remarkable efficacy, and is suitable for large-scale production.

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion / g to 1 billion / g and an organic matter contentof 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

The invention discloses an extraction method of holothurin, and belongs to the technical field of marine organisms. Sea cucumber deep processing waste sea cucumber viscera are used as raw materials; amodern biological separation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology and an ethanol precipitation and resin adsorption technology are integrated, a holothurian saponin biological product with high in-vitro oxidation resistance and an anti-cancer effect is developed, and mixed bacteria aerobic fermentation strains mainly comprise saccharomyces cerevisiae, bacillus subtilis and bacillus psychrosaccharolyticus. The obtained holothurian saponin has relatively strong antioxidant capacity and anti-cancer function, is strong in spectral property, and can be efficiently utilized in the fields of health care, foods, medicines and the like. The extraction method is high in holothurian saponin extraction rate, capable of achieving resourcereutilization of holothurian processing waste, convenient for industrial production and wide in market prospect.

The invention relates to a biological preparation for degrading pesticide residue in plants and a preparation method thereof, and relates to the technical field of agriculture production. The biological preparation solves the problems of poor pesticide degradation effects, low degradation speed and no broad spectrum of the existing microbial bacterial agents, and is prepared from a plant source pesticide mixture and a mixed bacterial solution according to the mixture ratio of 3:2, wherein the plant source pesticide mixture is prepared from the following materials in parts by weight: 1 to 5 parts of cortex meliae, 1 to 5 parts of radix sophorae falvescentis, 1 to 5 parts of hellebore and 1 to 5 parts of mustard seed, wherein the mixed bacterial liquid comprises the following ingredients inparts by weight: 20 to 30 parts of bacillus thuringiensis fermentation bacterial liquid, 10 to 15 parts of bacillus brevis lateralis fermentation bacterial liquid, 5 to 10 parts of bacillus psychrosaccharolyticus fermentation bacterial liquid, 5 to 10 parts of bacillus cereus fermentation bacterial liquid, 5 to 10 parts of yeast fermentation bacterial liquid, 5 to 10 parts of bacillus subtilis fermentation bacterial liquid, 5 to 8 parts of bacillus megatherium fermentation bacterial liquid, 30 to 35 parts of pseudomonas putidafermentation bacterial liquid and 25 to 30 parts of pseudomonas stutzeri fermentation bacterial liquid.

The invention discloses a preparation method of a functional biofertilizer. The functional biofertilizer comprises, by weight, 6 parts of isosulfocyanide, 8 parts of ammonium molybdate, 11 parts of salicylic acid, 4 parts of zinc sulfate, 10 parts of silicon oxide, 4 parts of boric acid, 1 part of bacillus psychrosaccharolyticus, 4 parts of monopotassium phosphate, 5 parts of ethyl valerate, 4 parts of copper sulfate, 2 parts of manganese sulfate, 1 part of bacillus megaterium, 1 part of magnesium sulfate, and 1 part of nitrifying bacteria. Fertilizer efficiency of the functional biofertilizer is improved; the functional biofertilizer is capable of controlling insect attack; and survival efficiency of microorganisms in the functional biofertilizer is increased.

The invention belongs to the field of marine biotechnology, and discloses a method for improving the anticancer immunoenhancement efficacy of a Baolingsu extracted solution. Abalone is used as the rawmaterial, a modern biological separation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology, and an ethanol precipitation and resin adsorption technology are integrated to research and develop the Baolingsu biological product with high antitumor immunoenhancement efficacy, wherein mixed aerobic fermentation strains mainly comprise bacillus subtilis, bacillus licheniformis and bacillus psychrosaccharolyticus, and glucan is added in the enzymolysis process, so that the anti-cancer immunoenhancement efficacy of the Baolingsu extracted solution isremarkably improved. According to the process technology, the extraction rate of Baolingsu is high, and the obtained Baolingsu biological product is high in anti-cancer capability and convenient for industrial large-scale production.

The invention discloses a compounded fermented abalone-sea cucumber biological product and belongs to the technical field of marine organisms. The abalone-sea cucumber biological product disclosed bythe invention with high immunity strengthening capability and the effect of invigorating the kidney and supporting Yang is researched and developed through taking abalones and sea cucumbers as raw materials, assisting the raw materials with chitosan, and integrating a modern bioseparation technology, a bio-enzymolysis technology, a mixed-bacterium microbial fermentation technology and a marine biological resource nutrition blending technology; a blending ratio of the raw materials is as follows: 100 parts of abalones, 10-20 parts of chitosan and 50-80 parts of sea cucumbers; and mixed-bacterium aerobic fermentation strains mainly comprise bacillus psychrosaccharolyticus, bacillus subtilis and bacillus licheniformis. The abalone-sea cucumber biological product disclosed by the invention isrich in a variety of functional active substances such as functional polypeptides, glycosaminoglycan, flavone and saponin, has a remarkable immunity strengthening effect, has remarkable kidney invigorating and Yang supporting effects, is a novel efficient abalone-sea cucumber biological product and has a broad market application prospect.

The invention discloses a process for preparing stomach-invigorating marine health product by a fermentation and enzymolysis method and belongs to the technical field of marine organisms. By couplingof the fermentation technology and the enzymolysis technology, stomach invigorating function of rare marine products such as abalones and sea cucumbers is improved, and combination of enhancing the immunity and invigorating the spleen and the stomach is achieved. With integration of the modern bioseparation technology, the biological enzymolysis technology, the mixed microbial fermentation technology and living marine resource nutrition blending technology, the stomach-invigorating marine health product capable of enhancing the human immunity and invigorating the spleen and the stomach is developed by taking the marine products such as abalones and sea cucumbers as the raw material with marine oligosaccharides as accessories according to the proportion of 100 parts of abalones, 2-5 parts of marine oligosaccharides and 50-80 parts of sea cucumbers; the mixed aerobic fermentation strain primarily comprises bacillus psychrosaccharolyticus, bacillus subtilis and bacillus cereus. The stomach-invigorating marine health product contains various functional active substances such as functional peptides, glycosaminoglycan, flavone and saponin, has excellent immunity-enhancing function and remarkable spleen and stomach invigorating function, and accordingly is a novel marine health biological product with broad market application prospect.

The invention relates to a method for producing a liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid. Alga powder is added into water according to a certain proportion, and sterilization is conducted at a certain temperature for a certain period of time after the materials are contained in a fermentation tank; a seed solution is prepared, wherein a fermentation strain, namely YIC-ALG3 saccharophilous bacillus, capable of degrading alginic acid is obtained through screening, and the strain is inoculated to a liquid culture medium to be cultured for a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio and fermented under certain conditions, fermentation conditions are adjusted after fermentation, after preservation is conducted for a certain period of time, the fermentation conditions are adjusted again and the seed solution is supplemented, saccharophilous bacillus filtrate is obtained through filter pressing after three times of adjustment, water, the saccharophilous bacillus filtrate, nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and organic substances containing humic acid are added into a stirring kettle, and the liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid is obtained after sufficient stirring and dissolving. The fermentation process is stable and controllable, the fermentation cycle is short, product effects are remarkable, and the method is suitable for large-scale production.

The invention discloses a microbial rotten bacterium agent as well as a preparation method and an application thereof. The microbial rotten bacterium agent is formed by mixing and compounding bacillus psychrosaccharolyticus, geobacillus stearothermophilus and bacillus thuringiensis in proportion. The invention further provides the preparation method of the microbial rotten bacterium agent. Based on the optimization combination of the bacillus psychrosaccharolyticus, the geobacillus stearothermophilus and the bacillus thuringiensis, the rotten time is shortened by 10 to 20 days; the deodorization is carried out effectively during the rotten process, so that the environment pollution is avoided; the fatality rate of pests and worm eggs can reach 100%. The rotten agricultural / animal wastes or enteromorpha can directly serve as base fertilizers tilled into the soil, so that the soil structure is improved; the soil fertility is enriched; beneficial bacteria in the soil are increased. Thus, infectious diseases spreading via the soil are avoided.

The invention relates to a preparation method of a seaweed compound fertilizer containing a saccharophilous bacillus microbial inoculum. The preparation method comprises the following steps of grinding oven-dried seaweed into powder, adding the powder of the seaweed into water according to a certain proportion, loading an obtained first mixture in a fermentation tank, and then sterilizing the first mixture for a certain time at a certain temperature; the preparation of a seed solution: sieving to obtain a fermentation strain YIC-ALG3 saccharophilous bacillus capable of degrading alginic acid, inoculating the fermentation strain YIC-ALG3 saccharophilous bacillus into a liquid nutrient medium, and culturing the fermentation strain YIC-ALG3 saccharophilous bacillus for a certain time; adding the seed solution into the fertilization tank according to a certain mass ratio, fertilizing an obtained second mixture in a certain condition, after fertilization, adjusting a fertilization condition, preserving the second mixture for a certain time, then adjusting the fertilization condition once again, supplementing the seed solution, obtaining a saccharophilous bacillus after three times of adjustment, and adding the saccharophilous bacillus into a compound fertilizer through an external wrapping way, so as to obtain the seaweed compound fertilizer containing the saccharophilous bacillus microbial inoculum. According to the preparation method, the fertilization process is stable and controllable; the fertilization period is short; the function of a product is obvious; the preparation method is applicable to large-scale production.

The invention relates to a method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid. Dried seaweed is ground into powder, the seaweed powder is added to water according to a certain proportion, and the mixture is placed in a fermentation tank and then sterilized at a certain temperature for a certain period of time; a seed solution is prepared; a fermentation strain YIC-ALG3 glycotropic bacillus capable of degrading alginic acid is obtained through screening, and a liquid culture medium is then inoculated with the fermentation strain for culture lasting a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio to be fermented under a certain condition, the fermentation condition is adjusted after fermentation, the fermentation condition is adjusted again and the seed solution is supplemented after a certain period time of storage, glycotropic bacillus is obtained after three times of adjustment, and glycotropic bacillus and organic matter containing amino acid are evenly stirred to obtain the water-soluble fertilizer containing glycotropic bacillus and amino acid. The fermentation process is stable and controllable, fermentation period is short, product functions are remarkable, and the fertilizer is suitable for large-scale production.

The invention relates to a method for inducing strains to participate in enzymolysis to produce alginate oligosaccharides and application of a product. The method comprises the following steps: brownalgae are subjected to enzymolysis by using bacillus halosaccharovorans, and ventilatory capacity, rotating speed and temperature are controlled in different time periods to improve the process, so that the brown algae are degraded to produce the alginate oligosaccharides with the polymerization degree of 2-6. According to the process, the yield of the alginate oligosaccharides can be effectivelyincreased, the polymerization degree of the alginate oligosaccharides can be stably controlled, the cost is reduced, the process is low-carbon and environmentally friendly, the production efficiency is improved, and the industrial production of the alginate oligosaccharides is realized; and meanwhile, the alginate oligosaccharides produced with the method can be used as animal feed additives, theutilization efficiency of the alginate oligosaccharides is improved, the immunity of animals is enhanced, the growth and reproduction of the animals are promoted, and the quality of the animals is further improved.

The invention discloses biological products for benefitting spleen, invigorating stomach and resisting ageing and a preparation technology of the biological products, and belongs to the technical field of marine organisms. Ballonfish skin is used as raw materials, a modern bioseparation technique, a biological enzymolysis technique and a mixed bacterium microorganism fermentation technique are integrated, so that biological products for notably benefitting spleen, invigorating stomach and resisting ageing are developed. Mixed bacterium aerobic zymocyte mainly comprises aspergillus oryzae and bacillus psychrosaccharolyticus. The obtained biological products for benefitting spleen, invigorating stomach and resisting ageing have high antioxidant ability. The obtained biological products are subjected to concentration, adsorption and drying, alcohol precipitation or resin purification and the like according to an application way, and can be in efficient utilization in the fields of healthcare, foods, medicines and the like. The biological products are simple in production technology, high in antioxidant ability and high in free radical eliminating ability, and have the health-care efficacy of notably benefitting the spleen, invigorating the stomach and resisting ageing. The products are high in stability and have broad market application prospects.