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Method for producing liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid

A technology of Bacillus saccharophilus and a production method, which is applied in the field of liquid water-soluble fertilizer production, can solve problems such as the inability to exert the effect of microbial agents, the inability to form a population effect, soil compaction and acidification, etc., and achieve remarkable product efficacy and stable and reliable fermentation process. The effect of controlling and promoting root growth

Inactive Publication Date: 2016-12-14
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Method for producing liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid
  • Method for producing liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid
  • Method for producing liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0043]Put seaweed powder and water into a fermenter according to the mass ratio of 2:100, sterilize at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 20 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 2:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 1:100 to obtain product B; the product B is fermented at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is After 7 days, product C was o...

Embodiment 2

[0046] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0047] Seaweed powder and water are put into the fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into the liquid medium and cultivate for 22 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 5:100. Add the seed liquid to the product A, wherein the mass ratio of the seed liquid to the product A is 5:100 to obtain the product B; the product B is fermented at a tem...

Embodiment 3

[0050] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0051] Put seaweed powder and water into a fermenter according to the mass ratio of 6:100, sterilize at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 24 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 8:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days , to obtain product C; adjust th...

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Abstract

The invention relates to a method for producing a liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid. Alga powder is added into water according to a certain proportion, and sterilization is conducted at a certain temperature for a certain period of time after the materials are contained in a fermentation tank; a seed solution is prepared, wherein a fermentation strain, namely YIC-ALG3 saccharophilous bacillus, capable of degrading alginic acid is obtained through screening, and the strain is inoculated to a liquid culture medium to be cultured for a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio and fermented under certain conditions, fermentation conditions are adjusted after fermentation, after preservation is conducted for a certain period of time, the fermentation conditions are adjusted again and the seed solution is supplemented, saccharophilous bacillus filtrate is obtained through filter pressing after three times of adjustment, water, the saccharophilous bacillus filtrate, nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and organic substances containing humic acid are added into a stirring kettle, and the liquid water-soluble fertilizer containing saccharophilous bacillus and humic acid is obtained after sufficient stirring and dissolving. The fermentation process is stable and controllable, the fermentation cycle is short, product effects are remarkable, and the method is suitable for large-scale production.

Description

technical field [0001] The invention relates to a production method of liquid water-soluble fertilizer. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturers are mainly brown algae, including Ascophyllum nodosum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C12N1/20C12R1/07C05G3/80
CPCC05F9/04C12N1/20C05G3/80C05F11/02C05F11/08
Inventor 王学江刘斌袁蕊迟艳
Owner WUZHOUFENG AGRI SCI & TECH
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