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Biological preparation for degrading pesticide residue in plants and preparation method thereof

A technology of pesticide residues and biological preparations, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problems of slow degradation, no broad-spectrum, and poor degradation of pesticides, and achieve Accelerated growth and development, significant degradation effect, and improved drug resistance

Inactive Publication Date: 2019-01-25
大连百事农生物有机肥研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor pesticide degradation effect, slow degradation speed and no broad spectrum existing in existing microbial bacterial agents, the present invention provides a biological agent for degrading pesticide residues in plants and a preparation method thereof

Method used

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Examples

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preparation example Construction

[0044] A method for preparing a biological agent for degrading pesticide residues in plants of the present invention, specifically comprising the following steps:

[0045] Step 1, preparation of botanical medicinal mixture

[0046] 1) Select and clean neem bark, flavescens, veratrum and mustard seeds respectively, then crush them to 10-50 mesh, mix them evenly, and obtain a mixed fine powder;

[0047] 2) Add the mixed fine powder into the reaction kettle, and then soak it in an acidic aqueous solution with pH 4.2-6.3 and temperature 40-75°C for 1-3 hours. The solid-liquid ratio of the mixed fine powder to the acidic aqueous solution is 1g: (3-7) ml;

[0048] 3) Using a subcritical water extraction method, extracting the substances in the reaction kettle under the conditions of 1-5Mpa and 100-150°C for 30-60 minutes, separating the substances in the reaction kettle, and obtaining a liquid plant-derived pharmaceutical mixture;

[0049] Step 2, preparation of mixed bacterial so...

Embodiment 1

[0065] The preparation of the biological preparation of the pesticide residue in the degraded plant of embodiment 1

[0066] 1) 3 kg of neem bark, 3 kg of sophora flavescens, 3 kg of veratrum, and 3 kg of mustard seeds are selected and cleaned respectively, then pulverized to 30 mesh, mixed uniformly, and mixed fine powder is obtained;

[0067] 2) Take 100g of mixed fine powder and add it to the reaction kettle, and then soak it in 500ml of acidic aqueous solution with pH 5.0 and temperature 55°C for 2h;

[0068] 3) Subcritical water extraction method is used to extract the substance in the reaction kettle under the condition of 3Mpa and 125°C for 45 minutes, and separate the substance in the reaction kettle to obtain a liquid plant-derived medicinal mixture;

[0069] 4) Prepare Bacillus thuringiensis seed liquid, Brevibacillus lateralosporus seed liquid, Bacillus coldolyticus seed liquid, Bacillus cereus seed liquid, yeast seed liquid, Bacillus subtilis seed liquid, Bacillus ...

Embodiment 2

[0072] Preparation of biological agents for degrading pesticide residues in plants in embodiment 2

[0073] 1) 1 kg of neem bark, 1 kg of sophora flavescens, 5 kg of veratrum, and 5 kg of mustard seeds are selected and cleaned respectively, then crushed to 50 mesh, mixed uniformly, and mixed fine powder is obtained;

[0074] 2) Take 100g of mixed fine powder and add it to the reaction kettle, and then soak it in 300ml of acidic aqueous solution with pH 6.3 and temperature 75°C for 3h;

[0075] 3) Using the subcritical water extraction method, extracting the substance in the reaction kettle under the conditions of 1Mpa and 150°C for 60 minutes, separating the substance in the reaction kettle, and obtaining a liquid botanical agent mixture;

[0076] 4) Prepare Bacillus thuringiensis seed liquid, Brevibacillus lateralosporus seed liquid, Bacillus coldolyticus seed liquid, Bacillus cereus seed liquid, yeast seed liquid, Bacillus subtilis seed liquid, Bacillus megaterium seed liqui...

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Abstract

The invention relates to a biological preparation for degrading pesticide residue in plants and a preparation method thereof, and relates to the technical field of agriculture production. The biological preparation solves the problems of poor pesticide degradation effects, low degradation speed and no broad spectrum of the existing microbial bacterial agents, and is prepared from a plant source pesticide mixture and a mixed bacterial solution according to the mixture ratio of 3:2, wherein the plant source pesticide mixture is prepared from the following materials in parts by weight: 1 to 5 parts of cortex meliae, 1 to 5 parts of radix sophorae falvescentis, 1 to 5 parts of hellebore and 1 to 5 parts of mustard seed, wherein the mixed bacterial liquid comprises the following ingredients inparts by weight: 20 to 30 parts of bacillus thuringiensis fermentation bacterial liquid, 10 to 15 parts of bacillus brevis lateralis fermentation bacterial liquid, 5 to 10 parts of bacillus psychrosaccharolyticus fermentation bacterial liquid, 5 to 10 parts of bacillus cereus fermentation bacterial liquid, 5 to 10 parts of yeast fermentation bacterial liquid, 5 to 10 parts of bacillus subtilis fermentation bacterial liquid, 5 to 8 parts of bacillus megatherium fermentation bacterial liquid, 30 to 35 parts of pseudomonas putidafermentation bacterial liquid and 25 to 30 parts of pseudomonas stutzeri fermentation bacterial liquid.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to a biological preparation for degrading pesticide residues in plants and a preparation method thereof. Background technique [0002] Since the Swiss Muller first discovered the insecticidal effect of DDT in 1938 and successfully used it to kill potato beetles, organochlorine pesticides began to appear in the development of pesticides. Since the 1940s, organochlorine pesticides such as DDT and HCH have been widely used in the control of plant diseases and insect pests, resulting in excessive pesticide residues in agricultural products and Chinese herbal medicines and environmental pollution, posing a serious threat to non-target organisms and human health. However, the pollution of organochlorine pesticides in crops has gradually emerged. The residues exceed the standard and the product price is low, which seriously restricts the export of crops in our country and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/32A01N65/42A01N63/02A01N63/04A01P21/00
CPCA01N25/32A01N63/10A01N63/30A01N65/00A01N65/08A01N65/20A01N65/26A01N65/42
Inventor 郝玉杰杨子涵
Owner 大连百事农生物有机肥研究所
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