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A kind of Bacillus saccharophilus for degrading alginic acid and its application method

A technology of Bacillus saccharophila and alginic acid, applied in the field of Bacillus saccharophila, can solve the problems of difficulty in meeting industrial production requirements, poor controllability of the fermentation process, and difficulty in guaranteeing product quality, and achieves remarkable product efficacy and stable fermentation process. Control, the effect of short fermentation cycle

Active Publication Date: 2019-08-13
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are relatively few studies on the use of microbial fermentation to produce seaweed fertilizers. There are only a few studies that use a variety of microorganisms to perform composting or aerobic fermentation on seaweed raw materials. Unclear problems, it is difficult to meet the unified, stable and controllable standards required by industrial production, and it is difficult to guarantee the quality of products

Method used

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  • A kind of Bacillus saccharophilus for degrading alginic acid and its application method
  • A kind of Bacillus saccharophilus for degrading alginic acid and its application method
  • A kind of Bacillus saccharophilus for degrading alginic acid and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0052] Seaweed powder and water are put into a fermenter according to the mass ratio of 2:100, and sterilized at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate the above-mentioned Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 20 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogenphosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 2:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 1:100 to obtain product B; ferment product B at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is 7 days; collecting the ferment...

Embodiment 2

[0054] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0055] Seaweed powder and water are put into a fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate the Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 22 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 5:100. The seed liquid is added to product A, wherein the mass ratio of seed liquid to product A is 5:100, and product B is obtained; product B is fermented at a temperature of 25°C and a ...

Embodiment 3

[0057] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0058] Seaweed powder and water are loaded into a fermenter according to the mass ratio of 6:100, and sterilized at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate the Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 24 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days ; Collecting the fermentation liquid ...

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Abstract

The invention relates to a Bacillus saccharophilus for degrading alginic acid and an application method thereof. The dried seaweed is ground into powder, and the seaweed powder is added into water according to a certain ratio, and after being put into a fermenter, at a certain temperature, a certain Sterilization under time; preparation of seed liquid: a fermentation strain YIC~ALG3 capable of degrading alginic acid was screened and inoculated into 2216E liquid medium for overnight cultivation; the seed liquid was added to the fermenter according to a certain mass ratio, and under certain conditions Fermentation, after fermentation, the raw material of brown algae is processed by microbial fermentation to prepare seaweed liquid. The fermentation process of the invention is stable and controllable, the fermentation period is short, the product has remarkable efficacy, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a bacillus saccharophilus for degrading alginic acid, and also relates to a method for preparing seaweed liquid by using the bacillus saccharophilus and a method for preparing fertilizer by using the bacillus saccharophilus. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G1/00C12R1/07
CPCC05B7/00C12N1/205C12R2001/07C05C3/00C05D1/02C05F5/00C05F5/008
Inventor 王明鹏王学江刘正一李峰秦松迟艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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