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Production method of water soluble fertilizer containing saccharophilous bacillus and humic acid

A technology of Bacillus saccharophila and production method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, organic fertilizers, etc., can solve problems such as inability to exert the effect of microbial inoculants, inability to form population effects, soil compaction and acidification, etc. Achieve significant product efficacy, stable and controllable fermentation process, and promote root growth.

Inactive Publication Date: 2016-12-07
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Production method of water soluble fertilizer containing saccharophilous bacillus and humic acid
  • Production method of water soluble fertilizer containing saccharophilous bacillus and humic acid
  • Production method of water soluble fertilizer containing saccharophilous bacillus and humic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0043]Put seaweed powder and water into a fermenter according to the mass ratio of 2:100, sterilize at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 20 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 2:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 1:100 to obtain product B; the product B is fermented at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is After 7 days, product C was o...

Embodiment 2

[0046] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0047] Seaweed powder and water are put into the fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into the liquid medium and cultivate for 22 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 5:100. Add the seed liquid to the product A, wherein the mass ratio of the seed liquid to the product A is 5:100 to obtain the product B; the product B is fermented at a tem...

Embodiment 3

[0050] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0051] Put seaweed powder and water into a fermenter according to the mass ratio of 6:100, sterilize at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 24 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 8:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days , to obtain product C; adjust th...

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Abstract

The invention relates to a production method of a water soluble fertilizer containing saccharophilous bacillus and a humic acid, wherein dried seaweed is grinded into powder, the seaweed powder is added into water at certain proportion, and is sterilized at certain temperature for certain time after packaged into a fermentation tank; a seed solution is prepared by screening to obtain a fermentation strain, saccharophilous bacillus YIC-ALG3, capable of degrading alginic acid, inoculating to a liquid medium and culturing for a period of time, adding the seed solution to the fermentation tank according to mass ratio, fermenting at certain conditions, adjusting the fermentation condition after fermentation, adjusting the fermentation condition again and supplementing the seed solution after storing for a period of time, and adjusting for three times to obtain the saccharophilous bacillus; the saccharophilous bacillus, a nitrogenous fertilizer, a phosphatic fertilizer, a potassic fertilizer and an organic matter containing the humid acid are evenly stirred to obtain the water soluble fertilizer containing the saccharophilous bacillus and the humic acid. The fermentation process is stable and controllable, the fermentation period is short, the effects of the product are remarkable, and the production method is suitable for scale production.

Description

technical field [0001] The invention relates to a production method of water-soluble fertilizer containing bacillus saccharophilus and humic acid. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturers are mainly...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05F11/08C12N1/20C12R1/07C05G3/80
CPCC05D1/00C05F11/08C12N1/20C05G3/80C12N1/205C12R2001/07
Inventor 王学江马宁李峰张志凯
Owner WUZHOUFENG AGRI SCI & TECH
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