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Preparation method of seaweed compound fertilizer containing saccharophilous bacillus microbial inoculum

A technology of Bacillus saccharophilus and compound fertilizer, applied in organic fertilizers, urea compound fertilizers, nitrogen fertilizers, etc., can solve the problems of soil compaction and acidification, inability to exert the effect of microbial agents, and inability to form population effects, etc., to promote root growth , The effect of the product is remarkable, and the fermentation process is stable and controllable

Active Publication Date: 2016-12-07
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Preparation method of seaweed compound fertilizer containing saccharophilous bacillus microbial inoculum
  • Preparation method of seaweed compound fertilizer containing saccharophilous bacillus microbial inoculum
  • Preparation method of seaweed compound fertilizer containing saccharophilous bacillus microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0044] Put seaweed powder and water into a fermenter according to the mass ratio of 2:100, sterilize at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 20 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 2:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 1:100 to obtain product B; the product B is fermented at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is After 7 days, product C was obtained; adju...

Embodiment 2

[0046] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0047] Seaweed powder and water are put into the fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into the liquid medium and cultivate for 22 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 5:100. Add the seed liquid to the product A, wherein the mass ratio of the seed liquid to the product A is 5:100 to obtain the product B; the product B is fermented at a temperature of 25...

Embodiment 3

[0049] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0050] Put seaweed powder and water into a fermenter according to the mass ratio of 6:100, sterilize at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 24 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 8:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days , to obtain product C; adjust the pH of produc...

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Abstract

The invention relates to a preparation method of a seaweed compound fertilizer containing a saccharophilous bacillus microbial inoculum. The preparation method comprises the following steps of grinding oven-dried seaweed into powder, adding the powder of the seaweed into water according to a certain proportion, loading an obtained first mixture in a fermentation tank, and then sterilizing the first mixture for a certain time at a certain temperature; the preparation of a seed solution: sieving to obtain a fermentation strain YIC-ALG3 saccharophilous bacillus capable of degrading alginic acid, inoculating the fermentation strain YIC-ALG3 saccharophilous bacillus into a liquid nutrient medium, and culturing the fermentation strain YIC-ALG3 saccharophilous bacillus for a certain time; adding the seed solution into the fertilization tank according to a certain mass ratio, fertilizing an obtained second mixture in a certain condition, after fertilization, adjusting a fertilization condition, preserving the second mixture for a certain time, then adjusting the fertilization condition once again, supplementing the seed solution, obtaining a saccharophilous bacillus after three times of adjustment, and adding the saccharophilous bacillus into a compound fertilizer through an external wrapping way, so as to obtain the seaweed compound fertilizer containing the saccharophilous bacillus microbial inoculum. According to the preparation method, the fertilization process is stable and controllable; the fertilization period is short; the function of a product is obvious; the preparation method is applicable to large-scale production.

Description

technical field [0001] The invention relates to a preparation method of seaweed compound fertilizer containing bacillus saccharophilus bacterial agent. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer made of natural seaweed as raw material, which is processed by special biochemical process to extract and retain the natural active ingredients of seaweed, refined and concentrated. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCC05B7/00C05G5/30C05C9/00C05D1/02C05D9/00C05F11/08
Inventor 王学江迟艳王明鹏李峰
Owner WUZHOUFENG AGRI SCI & TECH
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