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Microbial bacterial agent and preparation method thereof

A microbial agent and microbial technology, which is applied in the field of microbial agent and its preparation, can solve the problems of low control efficiency, environmental pollution, and unsafe chemical pesticides for humans and animals, and achieve no damage to the ecological balance, good disease prevention effect, and low The effect of chemical fertilizer dosage

Inactive Publication Date: 2018-10-23
JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems that chemical pesticides are unsafe to humans and animals, easily cause environmental pollution, and have relatively low growth-promoting and control effects, and provide an agricultural compound microbial bacterial agent with functions of promoting growth and preventing diseases and its The preparation method is to effectively combine botanical pesticides and biological bacteria agents, and use them as flushing or foliar fertilizers in vegetable planting, with good control effect, obvious growth promotion, safety and environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 fermentation liquid

[0043] Prepare Bacillus thuringiensis seed liquid, Brevibacillus lateralosporus seed liquid, Saccharomyces pyrolyticum seed liquid, Bacillus cereus seed liquid, yeast seed liquid, Bacillus subtilis seed liquid and Bacillus megaterium seed liquid respectively by using existing technology liquid;

[0044] Take the seed liquids of the above-mentioned various bacteria, use the existing technology to inoculate them into their respective culture substrates, and then culture them at 28°C at a shaking speed of 200r / min until the formation of spores and paraspore crystals, and the separation of crystal spores, respectively. Obtain the fermentation broth of the above various bacteria.

[0045] Wherein, the formula (in parts) of the culture substrate of various bacteria is as follows:

[0046] Bacillus thuringiensis: beef extract 1%; peptone 1%, agar 2%, sodium chloride 0.2%;

[0047] Brevibacillus sporogenes: Glucose 1.5%, ...

Embodiment 2

[0053] The preparation of embodiment 2 microbial bacterial agents

[0054] 1. Prepare the raw materials neem seeds, peel, sophora flavescens and veratrum and grind them respectively;

[0055]2. Add the crushed neem seeds, pericarp, flavescens, and veratrum into the reaction kettle according to the weight ratio of 2:3:3:2, and add the neem seeds, pericarp, flavescens, and veratrum to the weight of water Add water at a ratio of 1:3, adjust the pH to 6.3 with acetic acid, soak for 3 hours at a temperature of 40°C, and then use alcohol extraction to extract the substances contained in the neem seed peel, flavescens, and veratrum to obtain a mixed solution;

[0056] 3. Using the subcritical water extraction method, under the conditions of 1Mpa and 150°C, extract the material in the reactor for 30 minutes, and then separate the upper layer of the material in the reactor to obtain the natural extract component;

[0057] 4. According to parts by weight, take respectively 20 parts of ...

Embodiment 3

[0059] The preparation of embodiment 3 microbial bacterial agents

[0060] 1. Prepare the raw materials neem seeds, peel, sophora flavescens and veratrum and grind them respectively;

[0061] 2. Add the crushed neem seeds, pericarp, flavescens, and veratrum into the reaction kettle according to the weight ratio of 2:3:3:2, and add the neem seeds, pericarp, flavescens, and veratrum to the weight of water Add water at a ratio of 1:7, adjust the pH to 4.2 with acetic acid, soak for 1 hour at a temperature of 75°C, and then use alcohol extraction to extract the substances contained in the neem seed peel, flavescens, and veratrum to obtain a mixed solution ;

[0062] 3. Using the subcritical water extraction method, under the conditions of 5Mpa and 100°C, extract the material in the reactor for 60 minutes, and then separate the upper layer of the material in the reactor to obtain the natural extract component;

[0063] 4. According to parts by weight, take respectively 30 parts o...

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Abstract

The invention discloses a microbial bacterial agent and a preparation method thereof, and solve the technical problems that chemical pesticides are not safe on people and livestock, so that the environment pollution can be easily caused; the growth promotion and prevention effects are relatively low. The microbial bacterial agent and the preparation method belong to the technical field of microbial preparations. The microbial bacterial agent is prepared from microbial ingredients of bacillus thuringiensis fermentation bacterial liquid, brevibacillus laterosporus fermentation bacterial liquid,bacillus psychrosaccharolyticus fermentation bacterial liquid, bacillus cereus fermentation bacterial liquid, yeast fermentation bacterial liquid, bacillus subtilis fermentation bacterial liquid andbacillus megatherium fermentation bacterial liquid, and natural extract ingredients of bead tree seeds, fruit peel, radix sophorae flavescentis and hellebore grass. The preparation method has the advantages that the plant source pesticides and the microbial bacterial agent are effectively combined to be used as fertilization or leaf fertilizers to be used in vegetable planting; the prevention effect is good; the growth promotion is obvious; safety and environment protection are realized.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to a microbial preparation and a preparation method thereof. Background technique [0002] The extensive use of chemical pesticides and chemical fertilizers has caused serious environmental pollution and obstacles to agricultural cultivation, including the reduction of soil organic matter, the aggravation of soil salinization, the increase of resistance to diseases and insect pests, and excessive drug residues in agricultural products. Insect pests are one of the important reasons for crop yield reduction. First, reducing the harm of pests is an important way to increase the yield and quality of food and feed crops. [0003] According to statistics, the annual loss of global food and feed crops due to insect pests reaches 14%, and the economic losses directly caused by agricultural production are as high as hundreds of billions of dollars. Spraying chemic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/07C12R1/085C12R1/125C12R1/11
CPCC12N1/16C12N1/20
Inventor 朱博邹建国张中成崔朝贺徐贺隋原邹丽辉
Owner JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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