Plant resistance inducer containing neoagaro-oligosaccharides
A new technology of agar oligosaccharide and plant resistance, applied in plant growth regulators, plant growth regulators, animal repellents, etc., can solve the problem of less application of agar oligosaccharides, and improve the induction of plant resistance. , Improve the production capacity, improve the effect of the effect
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Embodiment 1
[0028] A kind of plant resistance inducer of new agar oligosaccharide, described plant resistance inducer is prepared by following steps:
[0029] (1) Preparation of Bacillus coldolyticus fermentation broth
[0030] Prepare a nitrogen-free solid medium, inoculate the Bacillus coldolyticus strain into the nitrogen-free solid medium for streak culture, and transfer the activated Bacillus coldolyticum on the nitrogen-free solid medium to the nitrogen-free liquid medium , culturing to obtain a first-level seed liquid, inoculating the first-level seed liquid into a seed fermentation tank, and culturing to obtain a second-level seed liquid; then inoculating the seed liquid into a fermentation medium for fermentation to obtain a fermentation liquid of Saccharomyces pyrolyticum; The composition of described solid nitrogen-free medium is sucrose 10g / L, potassium dihydrogen phosphate 0.6g / L, magnesium sulfate 0.4g / L, sodium chloride 0.15g / L, calcium carbonate 0.5g / L, agar 20g / L ; the p...
Embodiment 2
[0034] A kind of plant resistance inducer of new agar oligosaccharide, described plant resistance inducer is prepared by following steps:
[0035] (1) Preparation of Bacillus coldolyticus fermentation broth
[0036] Prepare a nitrogen-free solid medium, inoculate the Bacillus coldolyticus strain into the nitrogen-free solid medium for streak culture, and transfer the activated Bacillus coldolyticum on the nitrogen-free solid medium to the nitrogen-free liquid medium , culturing to obtain a first-level seed liquid, inoculating the first-level seed liquid into a seed fermentation tank, and culturing to obtain a second-level seed liquid; then inoculating the seed liquid into a fermentation medium for fermentation to obtain a fermentation liquid of Saccharomyces pyrolyticum; The composition of described solid nitrogen-free medium is sucrose 10g / L, potassium dihydrogen phosphate 0.6g / L, magnesium sulfate 0.4g / L, sodium chloride 0.15g / L, calcium carbonate 0.5g / L, agar 20g / L ; the p...
Embodiment 3
[0040] A kind of plant resistance inducer of new agar oligosaccharide, described plant resistance inducer is prepared by following steps:
[0041] (1) Preparation of Bacillus coldolyticus fermentation broth
[0042] Prepare a nitrogen-free solid medium, inoculate the Bacillus coldolyticus strain into the nitrogen-free solid medium for streak culture, and transfer the activated Bacillus coldolyticum on the nitrogen-free solid medium to the nitrogen-free liquid medium , culturing to obtain a first-level seed liquid, inoculating the first-level seed liquid into a seed fermentation tank, and culturing to obtain a second-level seed liquid; then inoculating the seed liquid into a fermentation medium for fermentation to obtain a fermentation liquid of Saccharomyces pyrolyticum; The composition of described solid nitrogen-free medium is sucrose 10g / L, potassium dihydrogen phosphate 0.6g / L, magnesium sulfate 0.4g / L, sodium chloride 0.15g / L, calcium carbonate 0.5g / L, agar 20g / L ; the p...
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