119results about How to "Good insect resistance" patented technology

The invention discloses a litsea cubeba oil microcapsule and a preparation method thereof, and belongs to the technical field of mouldproof and insecticidal capsules. The litsea cubeba oil microcapsule is characterized by being prepared by the complex coacervation of litsea cubeba oil taken as a core material, sodium alginate taken as a main wall material and an aqueous solution which contains 1% to 5% of chitosan and 2% to 5% of anhydrous calcium chloride and is taken as a coagulating bath solution, wherein the mass ratio of the sodium alginate to the litsea cubeba oil is 1:(1-4). The preparation method mainly comprises the following steps: firstly preparing an emulsion A of the sodium alginate and the litsea cubeba oil; then preparing the aqueous solution of the chitosan and the anhydrous calcium chloride as the coagulating bath solution B; dripping the emulsion A into the coagulating bath solution B; and solidifying the mixture for certain time and then filtering the mixture, thereby obtaining the litsea cubeba oil microcapsule. Thus, the litsea cubeba oil microcapsule which is high in litsea cubeba oil content, long in service time and good in mouldproof and insecticidal effect and the preparation method of the litsea cubeba oil microcapsule are provided. The litsea cubeba oil microcapsule is mainly used for performing mouldproof and insecticidal functions on grains as well as on clothes.

To provide an Italian rye grass with excellent characteristics, and in particular, excellent insect resistance and disease resistance, an endophyte, which is a filamentous endophytic fungus living together with a wild plant occurring in nature, is isolated and artificially grown, and made to live symbiotically in Italian rye grass by inoculating and infecting the grass with the artificially grown endophyte.

The invention discloses a mothproof veneer board, a mothproof faced veneer board and a manufacturing method thereof, relating to veneer boards used for industries of building materials, furniture, interior decoration, and the like. The mothproof performance of the existing veneer boards and faced veneer boards is reduced because of reducing the usage amount of free formaldehyde. In the invention,adhesive containing a pyrethroid moth proofer is coated on the surface of a dried veneer, and then processes of assembly, pressing and molding are carried out, wherein the pyrethroid moth proofer is in a microcapsule shape, and the weight ratio of the pyrethroid moth proofer to the adhesive is (0.15-1.0):100. An obtained product has good mothproof performance and long service life.

The invention relates to the technical field of special sections required by manufacturing of building doors and windows, in particular to a wood-plastic co-extruded section for doors and windows, which is integrally formed by a PVC-U(polyvinyl chloride unplasticized) plastic section and a wood-plastic section. Wood-plastic section raw materials and PVC-U plastic section raw materials are dried in a drier at a temperature of 70-80 DEG C, then sent into a wood-plastic extruder and a PVC-U section extruder respectively to be gradually heated at an initial temperature of 110-120 DEG C, and melted and plasticized at a temperature of 175-180 DEG C in a main unit metering section, the materials synchronously extruded by two plastic extruders are inputted into a composite co-extruding section forming mould for forming at a temperature of 175-190 DEG C, then subjected to vacuum forming in a vacuum forming mould before water cooling forming in a vacuum water tank at a temperature of 5-15 DEG C, and finally subjected to drawing, cutting and conventional subsequent processing procedures to make the wood-plastic co-extruded section. By means of wood indoors and plastic outdoors, each performance index and heat insulation performance required by windows and doors are improved and the gap in the technical field of window-door special section plastic-wood composite can be filled.

The invention provides a corn and soybean narrow strip channel intercropping planting method. The method comprises the following steps that corn and soybeans are sown at the same time, an intercropping planting mode is adopted to the corn and the soybeans, every 2-3 rows of the corn form a corn planting row, 3-5 rows of the soybeans are planted between every two adjacent corn planting rows, and the soybeans and the corn are the same in the planting direction; the corn row spacing, the soybean row spacing and the distance between the adjacent soybeans and the corns are equal. By scientifically and reasonably determining the planting distance of the corn and the soybeans, interaction between the corn and the soybeans is enhanced, the radiation light condition needed by the corn and the soybeans in the intercropping group are met fully, and therefore the purpose of increasing the yield of the corn and the yield of the soybeans is achieved.

The invention discloses a Foeniculum vulgare cultivation method. The Foeniculum vulgare cultivation method includes the following steps: a first step, selecting a land, turning up the land, and applying a fertilizer; a second step, soaking seeds of Foeniculum vulgare; a third step, using a drill seeder to sow the processed seeds of the Foeniculum vulgare in the second step to the land covered by amulching film in the first step; a fourth step, watering the Foeniculum vulgare in a day and night alternative manner watering manner after the Foeniculum vulgare germinate, wherein a spraying watering manner is adopted at daytime, and an atomizing watering manner is adopted at nighttime; spraying leaves with a foliage fertilizer during the 2-4 leaf period of seedlings of the Foeniculum vulgare;thinning the seedlings timely after all the seedlings of the Foeniculum vulgare emerge, wherein two parallel Foeniculum vulgare plants should be thinned into a single Foeniculum vulgare plant, the plant spacing is about 5 cm, and seedling thinning and weeding are performed. The Foeniculum vulgare cultivation method can perform management from seed processing, can improve the germination rate of the seeds through scientific processing, can shorten the germination period of the seeds of the Foeniculum vulgare, and can increase the yield of the Foeniculum vulgare; and the foliage fertilizer is applied to the Foeniculum vulgare, thereby promoting microelement absorption of the Foeniculum vulgare and improving the nutrimental value of the Foeniculum vulgare.

To provide an Italian rye grass with excellent characteristics, and in particular, excellent insect resistance and disease resistance, an endophyte, which is a filamentous endophytic fungus living together with a wild plant occurring in nature, is isolated and artificially grown, and made to live symbiotically in Italian rye grass by inoculating and infecting the grass with the artificially grown endophyte.

The invention relates to an excrement insect culture device capable of automatically separating insects from excrement and a culture method thereof. The device and the method are used for culturing maggot excrement and insect excrement in a large amount and capable of achieving automatic separation of insect excrement. The culture device mainly comprises a culture pool, the bottom of the culture pool is of a gravel cement structure, the peripheral wall of the culture pool is of a brick mixing structure, the pool face of the culture pool is provided with a slope, two sides are high, and the middle is low. A water discharge pipe as long as the culture pool is embedded under the low middle of the culture pool, sewage discharge holes vertically connected with the water discharge pipe are arranged above the water discharge pipe at intervals, and an insect collection bag capable of leaking water is sleeved in the sewage discharge holes. A row of plate blocks are arranged above the middle water discharge pipe of the culture pool, the laying length of the plate blocks is identical with that of the culture pool, and excrement is arranged in ridge in the culture pool. The excrement insect culture device has the advantages of reducing device construction cost of insect culture by excrement, reducing employment cost, improving insect breeding efficiency and being high in space utilization ratio, simple in structure, capable of conducting mechanical large-scale production, capable of automatically separating insect and excrement and conveniently collecting insects, easy in cleaning and the like.

The invention relates to fire-resistant imitated 'Xuan paper'. The fire-resistant imitated 'Xuan paper' comprises an ultra-long hydroxyapatite nanowire, an inorganic fiber and a nano-scale inorganic adhesive; the diameter of the ultra-long hydroxyapatite nanowire is 2-100nm, and the length thereof ranges from 20 micrometers to 2 millimeters; the diameter of the inorganic fiber is 1-40 micrometers,and the length thereof is 1-12mm; the particle diameter of the nano-size inorganic adhesive in the form of nanoparticles is 5-100nm.

The present invention provides a RNA interference vector and an application thereof on genetically modified nematode-resistant plants, which belong to the technical field of plant protection. The RNA interference vector is characterized in that a plant expression vector contains a nucleotide sequence which is more than 74 percent similar to a sequence shown by Sequence No:1 and has the same function as the protein expressed by the Sequence No:1 sequence does, and the nucleotide sequence is inserted into the plant expression vector in the positive and negative directions. The constructed vector transforms a receptor plant; the transcribed exogenous gene can form a foldback double-stranded hairpin loop RNA structure; after the genetically modified plant is eaten by nematode, the target gene in the body of the nematode, MiMPK1 gene, interacts with the dsRNA expressed by the exogenous gene in the body of the plant, so that a silencing effect occurs; and as a result, the nematode cannot normally develop and breed. Thus the effect of inhibiting the dissemination of nematode to protect crops is achieved.

A preparation method for a carbon-plastic fiber composite material comprises the steps: (1) selecting carbon powder, baking to dry and storing at a dry place before mixing, and selecting plastic coming from one of high density polyethylene, polyvinyl chloride, polypropylene, PET, ABS and ultrahigh molecular weight polyethylene; (2) getting carbon powder, thermoplastic plastic, an auxiliary agent and basalt fiber according to the ratio of 80:16:3:1, 70:26:3:1, 60:36:3:1 or 50:46:3:1; and (3) pouring the mixture obtained in the step (2) into an extruder, conveying hot blank plates formed through extrusion by a rotating conveyor, and cooling and cutting during conveying. The material is wide in raw material source, simple in manufacturing technology, simple in equipment, low in cost, stable in dimension, excellent in flame resistance, resistant to mildew and corrosion, good in pest resistance and recyclable.

The invention relates to a building material for home furnishing, in particular to a method for manufacturing a moth-proof and low-formaldehyde wardrobe plate. The method for manufacturing the moth-proof and low-formaldehyde wardrobe plate is characterized by comprising the steps of lathing a plank into pieces, conducting drying, trimming edges, applying adhesive, conducting prepressing, conducting hot-pressing, sanding cut edges, conducting moth proofing treatment, attaching abrasion resistant paper, conducting decoration veneering and sealing the edges. According to the method for manufacturing the wardrobe plate, the formaldehyde residual amount is small, an aluminum alloy frame is inlaid at the periphery of the wardrobe plate, moisture in the air can be effectively prevented from permeating the plate, the moisture resistance and water resistance of the wardrobe plate are improved, the wardrobe plate obtained through the manufacturing method has good moth prevention performance, mold prevention performance, moisture protection performance and abrasion resistant performance, the formaldehyde residual amount is small, damage to a human body is reduced, the obtained wardrobe plate is stable in quality, the dimension stability is good, the plate is not prone to being cracked, damaged or deformed, and the service life is long.

The invention discloses an early-maturing high-ginning-outturn cotton line breeding method which comprises the steps: A, utilizing early-maturing gene resources to breed an early-maturing novel material in a growth period: performing middle 5168 parent selection of an early-maturing variety; B, increasing spacetime selection pressure and breeding a very early-maturing cotton novel material with high pre-frost yield, high yield and stable yield; 1) increasing time selection pressure; 2) increasing space selection pressure; C, a breeding method in which low-generation mixed selection, high-generation single selection and Hainan island progeny planting are combined: 1) performing low-generation mixed selection; 2) performing high-generation single selection; D, a molecular marker screening method of middle 5168: utilizing identified SSR markers of HAU3318, MGHES-73 and NAU3913 related to early-maturing characters to distinguish the middle 5168 from short season cotton varieties like middle 425, China cotton research institute 16, China cotton research institute 36 and China cotton research institute 74 and spring cotton varieties like deltapine cotton 15, Shandong research center 28,China cotton research institute 12, China cotton research institute 41, China cotton research institute 45 and Shandong cotton research center 1. The method has the advantages of easiness, practicability, convenience in operation, quick selection speed and high selection efficiency; the selected novel variety has the characteristics of early maturity and high ginning outturn.

The invention relates to the field of edible mushroom breeding, in particular to an oyster mushroom breeding method. The oyster mushroom breeding method includes the following steps that oyster mushroom spores are collected and sown and grow into oyster mushroom fruit bodies in the mature period, mushroom context tissues are cut from the oyster mushroom fruit bodies for cultivation, then hyphae are obtained, the hyphae are cut into slices for cultivation, and the obtained hyphae are oyster mushroom cultures. According to the oyster mushroom breeding method, the hyphae are changed by a physical technological means, the hyphae meet the requirement gradually, and differentiation of the fruit bodies is decreased; the prepared cultures are used for cultivation, good disease resistance and insect resistance are achieved, and the oyster mushroom fruit bodies obtained by planting are thick in hull, deep in color, big, fleshy, not prone to being over ripe, glossy, shiny, rich in mushroom flavor and delicious in taste; the biotransformation efficiency of the first crop of oyster mushrooms reaches up to 70%, differentiation of the fruit bodies is effectively reduced by 30%, yellow mushrooms and dead mushrooms are avoided in the whole growth process, bud removal is avoided, and labor is saved.

The invention discloses a planting method for pollution-free green paddy. Weeding, intertillage, fertilizing on the paddy are conducted through a method to breed ducks in a paddy field, and green, pollution-free rice is planted. The ducks are bred with the weed in the field and insects as fodder, and the water in the paddy field and the characteristics that the ducks can splash in the water and ducks can eat the weed are utilized, so that the planting industry and the breeding industry are bonded organically, and a growing environment in which the paddy and the ducks are mutually complementary and beneficial is constructed. The rice which is good in taste and safe for eating is facilitated to produce, so that it is an efficient way for producing pollution-free paddy to breed ducks in the paddy field.

The present invention discloses a water retention fertilizer for peanut planting, wherein the water retention fertilizer is characterized in that raw materials comprise, by weight, 8-10 parts of urea, 20-30 parts of tea leaf residue, 8-12 parts of iron slag, 3-5 parts of a filler, 5-7 parts of plant ash, 0.6-0.8 part of abamectin, 0.3-0.5 part of an microorganism bacterial agent, 2-4 parts of potassium chloride, 2-4 parts of magnesium sulfate, 3-5 parts of zinc nitrate, 2-4 parts of manganese phosphate, 1-2 parts of ferrous sulfate, 0.8-1.2 parts of copper sulfate, 3-4 parts of boric acid, 2-3 parts of sodium tripolyphosphate, 1.2-1.4 parts of lysine, 3-5 parts of humic acid, and 3-5 parts of an auxiliary agent. According to the present invention, the water retention fertilizer has beneficial effects of soil structure improving, soil gas permeation improving, soil fertility improving, and soil water retention capacity improving; the abamectin in the adhesive provides the significant effects of insect disinfestation and pest prevention so as to substantially reduce the harm of nematodes; and the water retention fertilizer further has effects of soil pH value balance adjusting, plant root microenvironment improving, plant disease resistance and stress resistance enhancing, reasonable nutrient ratio, high fertilizer efficiency, significantly-improved crop fertilizer absorption, and achievement of strong disease resistance and stress resistance of roots.

The invention discloses an efficient, corrosion-resisting and mothproof wicker sol-gel surface treating agent containing traditional Chinese medicines and used for wickerwork. The efficient, corrosion-resisting and mothproof wicker sol-gel surface treating agent containing the traditional Chinese medicines and used for the wickerwork is prepared by the following raw materials including, by weight, 1-2 parts of fructus xanthii, 1-2 parts of radix stemonae, 1-2 parts of peppers, 0.5-1 part of prunella vulgaris, 1-2 parts of rhubarb, 0.5-1 part of mangnolia officinalis, 0.5-1 part of honeysuckle, 1-2 parts of chinaberry seeds, 3-5 parts of ethyl orthosilicate, 0.1-0.15 part of octadecyltriethoxysilane, 1-2 parts of boric acid, 10-15 parts of absolute ethyl alcohol, 0.1-0.2 part of polysorbate-80 and 80-100 parts of water. The wicker surface treating agent is good in corrosion-resisting performance and mothproof performance, high in effect continuity, capable of effectively prolonging the service life of the wickerwork artware and expanding the application range of the wickerwork artware and environment-friendly.

The invention discloses a brown planthopper gene Nl19243 as well as an encoding product and application thereof. The gene Nl19243 has the DNA sequence shown in SEQ ID No. 1. The DNA sequence is composed of 787 nucleotide bases and contains a complete encoding frame composed of 654 nucleotides, and the coded proteins contains a small-molecular protein with 217 amino acid residues. Research finds that the gene is related to the survival of brown planthopper. An RNAi vector for the Nl19243 is constructed and then is transferred into rice to express dsRNA of the gene Nl19243 in a transgenic rice body, so as to achieve the purpose of improving the resistance of rice to brown planthopper. The brown planthopper gene Nl19243 can be widely applied to crop breeding, particularly breeding of insect-resistant rice.

An insect-proofing and antibacterial finishing agent for fabric comprises the following raw materials by weight: 10-12 parts of tetrachloro dihydroxy triphenylmethane sodium sulfonate, 5-8 parts of ethyl p-hydroxybenzoate, 6-8 parts of 2-[2-(2-aminophenoxy)ethoxy]phenylamine, 1-5 parts of sucrose fatty acid ester, 50-70 parts of ethylene glycol and 68-74 parts of water. The sucrose fatty acid ester is sucrose laurate or sucrose dilaurate. The insect-proofing and antibacterial finishing agent for fabric provided by the invention has substantial pest control effect, and can effectively repel insects and protect the integrity of the fabric. The invention also has good antibacterial property, and can inhibit the growth of bacteria and mold; and the invention has reasonable formula, no toxic or side effect on the human body, and can be popularized and applied.

The invention relates to a method for producing white wash wood floor, wherein it comprises A, selecting floor blank material, that using the ones whose water content lower than 12%; B, shaping the blank that boring the blank into smooth plate, opening groove on it into semi-product; C, baking finish that baking finish on the surface; D, oil process that processing oil high-temperature wood fiber treatment; E, distantly reducing temperature, and checking water quality and packing the product. The invention can fully utilize the character of wood fiber cell, while the floor can absorb water quickly, without deformation.

To provide a plant not showing livestock toxicity due to symbiotic fungi with which it is infected, and having strong resistance to pests, symbiotic fungus comprising a filamentous fungus belonging to the genus Neotyphodium, wherein a filamentous fungus whose final metabolic product is chanoclavine, is artificially introduced into a plant to infect the plant.

The invention relates to a Chinese cabbage planting method. The Chinese cabbage planting method includes the following steps that 1, seeds are selected and pretreated; 2, the seeds are thoroughly flushed with clear water and then are subjected to soaking treatment by using an ammonium bicarbonate solution; 3, the seeds treated in the step 2 are dried at the temperature of 35-38 DEG C till the moisture content reaches 60-62% and are cultured at the temperature of 22-25 DEG C for 2 days; 4, the seeds treated in the step 3 are planted in land, the seed burying depth is 0.5-1 cm, and watering is performed by using the sodium bicarbonate solution 2 days later after planting; 5, weeding and insect prevention operations are performed after seed budding. According to the Chinese cabbage planting method, the treatment starts from seed treatment, adopts different treating agents for seeds and is carried out at different temperatures, the germination rate of Chinese cabbages is improved, and the nutritional value of the Chinese cabbages is improved.

The invention relates to an orchidaceae plant three-dimensional multi-layer cultivation device comprising more than two vertical cultivation barrels arranged in a stacking mode from bottom to top and partition frames supported between each two vertically adjacent vertical cultivation barrels. A barrel inner cavity of each vertical cultivation barrel is a compost cavity used for containing culture media, and a barrel body side wall of each vertical cultivation barrel is provided with a seedling planting hole penetrating through the barrel body side wall so that each compost cavity is communicated with the outside. The orchidaceae plant three-dimensional multi-layer cultivation device can timely adjust the height position and the horizontal angle position of planting according to environment requirements such as light, humidity and temperature required by growth of orchidaceae plants such as dendrobium, facilitates planting management such as watering and fertilizing, and is beneficial for improvement of product quality and planting efficiency.

The present invention discloses a composing prescription of a compound ivermectin injection and a preparation method of the compound ivermectin injection, and belongs to the veterinary medicine technology. The compound ivermectin injection comprises main drugs, a solvent and an antioxidant so as to prepare the stable high-concentration compound ivermectin injection. The injection has characteristics of advanced technology, good stability, broad spectrum, high efficiency, low irritation on the injected site, easy use, and clinical efficiency superior to the single-formula injection. According to the present invention, the raw materials comprise 0.5-5.0% of ivermectin, 2-20.0% of mebendazole, 0.5-5.0% of levamisole hydrochloride, 0.1-1% of the antioxidant, and the balance of the solvent, wherein the antioxidant is one or a mixture selected from thiourea, L-cysteamine hydrochloride, vitamin C, ethylenediamine, sodium hydrogen sulfite, anhydrous sodium sulfite, sodium formaldehydesulfoxylate dihydrate, sodium metabisulfite and the like, and the solvent is one or a mixture selected from propylene glycol, ethanol, glycerol, glycerol formal, dimethylformamide, dimethylacetamide, 2-pyrrolidone and the like.

The fragrant coloring tomb filling material consists of lime 80-95 wt%, realgar 0.5-5 wt%, sulfur 0.5-5 wt%, dry lacquer 0.5-5 wt%, pricklyash 0.5-5 wt%, atractylodes rhizome 0.5-5 wt%, white calamus 0.5-5 wt%, basket kern 0.5-5 wt% and perfume 0.5-10 wt%. The perfume is saffron, methanol, fennel, sandalwood, laurel, benzoin, eagle wood, dalbergia wood or their arbitrary combination. The present invention has the preserving, mothproof, moisture-proof and dirty-avoiding functions.

The invention provides a method for preparing fruit growth bagging paper by utilizing tobacco stalk, including the following steps: (1) soaking tobacco stalk; (2) making tobacco stalk pulp; (3) mixing and pulp blending; (4) wet end moulding; (5) dehydration drying. The fruit growth bagging paper (fruit-bagged paper) prepared by the invention has the advantages of high air permeability, high dry / wet strength, good waterproof performance, good shading performance and difficult black drainage, the problem that the air permeability of the existing fruit-bagged paper is lower is solved, the paper can be widely applied to implantation cultivation of various fruit, fruit anti-pollution protection can be realized, and colour and lustre of fruit is maintained to be fresh.

The invention provides date wood soaking treatment fluid which is prepared from, by weight, 30-40 parts of iodine, 2-3.8 parts of soybean peptide, 2-3.8 parts of anthocyanin, 0.5-1 part of lithium carbonate, 0.2-0.3 part of zinc oxide, 4-6 parts of dried ginger, 4-6 parts of white pepper, 5-10 parts of citrus aurantium, 2-3 parts of trimethyl silicone oil, 5-10 parts of a mulberry leaf extract, 5-10 parts of snowbell-leaf tickclover herbs, 2-4 parts of rhizoma atractylodis and 5-8 parts of an anti-corrosion preparation. The treatment fluid does not contain irritant chemicals, can permeate into date wood completely, fully kills worm eggs and infectious microbe in the date wood, and does not destroy wood textures of the date wood, special fragrance of the date wood and the dark red color of the date wood, the soaked date wood is easy to dry, after the date wood is dried, the air-dry density of the date wood is hardly changed, and the soaked date wood is very good in mothproof performance and anti-mould performance.

The invention relates to a textile fabric which is prepared by blending and weaving the following fiber in parts by weight: 30 to 50 parts of bamboo fiber, 20 to 40 parts of modal fiber, 10 to 20 parts of chitosan fiber, 20 to 30 parts of viscose fiber and 3 to 8 parts of a mildew-proof agent. The textile fabric disclosed by the invention has the advantages that the textile fabric is prepared by blending according to special raw material ratios, so that the characteristic of each raw material fiber is highlighted, and the textile fabric integrates the advantages of all the raw materials; therefore, the fabric prepared according to the formula is high in acid and alkali resistance, mildew resistance, worm damage resistance and weather resistance; clothes prepared from the textile fabric is soft, skin-friendly, high in drapability, cold-proof, warm, firmly combined, high in durability and bright in color and has certain market value, and people feel comfortable when wearing the clothes.

The invention discloses a pesticide application device suitable for putting of an unmanned aerial vehicle and a pesticide bag matched with the same in use and belongs to the field of agricultural pesticide application machinery. The pesticide application device comprises a machine body, a driving shaft, a blade impeller, a worm wheel and worm direct-current speed reducing motor, a laser igniter, amicroswitch and a control system. The machine body comprises a pesticide bag storage box and a pesticide bag putting box. The blade impeller is installed on the driving shaft and is arranged in the pesticide bag putting box. The worm wheel and worm direct-current speed reducing motor is connected with the driving shaft. The laser igniter is used for igniting a leading wire of the pesticide bag, and the microswitch is used for controlling the blade impeller to stop rotating. The control system is used for controlling pesticide bag putting. The pesticide bag comprises a bag body, a powdery pesticide and a firecracker. The powdery pesticide and the firecracker are accommodated in the bag body. The pesticide bag is put by the pesticide application device, explodes in the air and is scatteredon woods and fruit trees, and the problem that pesticide application of high and big woods or fruit trees is difficult is solved.

The invention belongs to the technical field of biology, and particularly relates to a marine nannochloropsis oculata transcriptional activation CRISPRa system and a construction method and application thereof. The system vector contains a Cas9 inactivated protein, a transcriptional activation effect protein VP64, a resistance selection marker gene, at least one gRNA scaffold sequence and a specific sequence for enhancing transcriptional activation (SunTag sequence). The invention also provides the application of the CRISPRa vector for the transcription activation of the nannochloropsis oculata gene in gene transformation. The invention provides a new model and a feasible method for gene function research of nannochloropsis oculata.