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Soybean non-tissue culture plant regeneration method and application thereof

A technology for regenerating plants and plants, which is applied in the field of soybean non-tissue culture plant regeneration, can solve the problems of unrealistic, long cultivation cycle, huge manpower and material resources, etc., and achieve cost reduction, high seedling rate and simple conditions Effect

Inactive Publication Date: 2011-01-19
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the irreversible and destructive damage caused by diseases, insect inoculation and cutting part of plant tissues or organs, the tested plants cannot reproduce
Usually, the identification of such materials or the detection of physiological and biochemical indicators need to wait until their offspring are genetically stable, but this often requires 3-5 generations of breeding time, and the derived strains of each offspring must be kept. If the plant produces 10 seeds per generation, then after 3 generations, the first seed will have 1,000 offspring plants. If the identification of disease and insect inoculation or physiological and biochemical analysis are carried out at this time, the scale of the experiment will undoubtedly be greatly increased. At the same time, it consumes huge manpower and material resources, and it is very unrealistic to inoculate diseases and insects on a large scale
Another way for plants to reproduce is through asexual reproduction, that is, tissue culture, but tissue culture requires strict aseptic conditions and culture conditions, and the regeneration of different varieties has a strong genotype dependence, and the culture period is longer than normal for plants. growth cycle

Method used

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  • Soybean non-tissue culture plant regeneration method and application thereof
  • Soybean non-tissue culture plant regeneration method and application thereof
  • Soybean non-tissue culture plant regeneration method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, non-tissue culture regeneration soybean plant

[0034] method one:

[0035] 1) Germination of seeds:

[0036] Select plump soybean seeds (Glycine max L.) without disease spots (purchased from Wuhan Zhongyou Tianlong Seed Industry Technology Co., Ltd.), soak in water for 10 hours, and the amount of seeds and water is 1g: 10ml. After the seeds are completely imbibed, put The seeds are spread on a 15×40CM paper tape, and a layer of absorbent paper is laid on the paper tape. The seeds are laid in a row along the long side of the paper tape, about 2 cm away from the paper tape, and the distance between the seeds is 2 cm. Each paper tape is laid 15-20 seeds, then roll up the paper tape, put the paper roll into a 40×30×15CM plastic box, place 40-50 rolls in each box, add water to the box to a height of about 2CM, store at 26°C, and light for 16 hours / 8 hours dark, light intensity 100μmol / m 2 Cultivate under the condition of / s, change the water once every 2 d...

Embodiment 2

[0066] Embodiment 2, carry out detection experiment with non-tissue culture regeneration soybean plant

[0067] The soybean seed Zhongdou 29 (purchased from Wuhan Zhongyou Tianlong Seed Industry Technology Co., Ltd.) was taken, and the method of Example 1 was used to cultivate Zhongdou 29 to obtain explants.

[0068] 1. Non-tissue culture regenerated soybean plants inoculated with soybean mosaic virus

[0069] 1) The explant grows the first compound leaf

[0070] The explants obtained above were cultivated in sterile vermiculite for 14 days, and water was often sprayed to keep them moist until the first compound leaf grew out of the explants; the culture conditions were 26°C, 16 hours of light / 8 hours of darkness, light intensity 100μmol / m 2 / s, the humidity RH is 60%.

[0071] 2) Inoculation identification of soybean mosaic virus:

[0072] With the diseased soybean leaf of soybean mosaic virus disease (pathogenic bacteria is potato Y virus family (Potyviridae), potato Y v...

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Abstract

The invention discloses a soybean non-tissue culture plant regeneration method and an application thereof. The provided method for culturing regeneration plants comprises the following steps of: removing the terminal bud and the root of a plantling, longitudinally cutting hypocotyl through equal division along a joint of cotyledon, and dividing one plantling into two plantlings to be respectively used as an explant, wherein each explant is provided with one cotyledon and one axillary bud at a cotyledon node; culturing the explants to obtain the regeneration plants. The plantling has the structure that two cotyledons and two axillary buds are located at the cotyledon node, and the terminal bud and an epicotyl for connecting the cotyledon node and the terminal bud are located above the cotyledon node, and the root and the hypocotyl for connecting the root and the cotyledon node are located below the cotyledon node. Experiments show that the method has the advantages of simple manufacturing for preparing the regeneration plants, low cost, high working efficiency and environmental protection.

Description

technical field [0001] The invention relates to a soybean non-tissue culture plant regeneration method and its application. Background technique [0002] In soybean disease-resistant and insect-resistant breeding, it is necessary to carry out disease and insect inoculation identification on the collected variety resources, hybrid offspring, and transgenic offspring, and to cut out certain tissues or organs of the plant for physiological and biochemical analysis. At the same time, it is required that the tested plants can be normal of offspring. The common feature of this kind of material is that the number of individuals is small, the genetic background of each individual is different, and it is difficult to obtain repeatedly in reality. Due to the irreversible and destructive damage caused by diseases, insect inoculation and cutting part of plant tissues or organs, the tested plants cannot reproduce. Usually, the identification of such materials or the detection of physio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 单志慧沙爱华陈海峰陈李淼郝青南孙佃臣田星星周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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