A method to promote efficient germination of rare and endangered sugar beet tree seeds

Abstract

The invention discloses a method for promoting efficient germination of seeds of a valuable and rare endangered plant yunnanopilia longistaminea, wherein the method comprises the steps: collecting ripe yunnanopilia longistaminea seeds; removing seed coats, and breaking inhibition of the seed coats; carrying out cleaning and disinfection treatment on the seeds; carrying out root promoting culture, and soaking the seeds with a 50 mg / L GA3 solution for 24 h; soaking the seeds with a 100 mg / L GA3 solution for 24 h, placing the seeds at the temperature of 5 DEG C, and refrigerating for 7-14 days to change endogenous hormone change of the yunnanopilia longistaminea in seed germination and seedling building processes, so as to break epicotyl dormancy; placing the seeds in the environment of the temperature of 28 DEG C, and culturing; culturing for 3-4 weeks to obtain seedlings having euphylla grown; and providing the seedlings for wild transplanting after growing for 8-10 weeks. The system method directly adopting the yunnanopilia longistaminea seeds for fast and efficient cultivation of the seedlings effectively relieves the epicotyl dormancy of the yunnanopilia longistaminea, ensures that the germination rate of the yunnanopilia longistaminea seeds is more than or equal to 70%, effectively shortens the seedling culture time by 60 days, and is simple and convenient to operate, high in survival rate, and strong in practicability. An effective technical means is provided for species protection biology, restoration ecology, resource exploitation and utilization and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting efficient germination of rare and endangered beet tree seeds. Background technique [0002] beet tree ( Yunnanopilia longistaminea ) belongs to the monotypic species of the genus Yunnanopilia of the family Opiliaceae, and is mainly distributed in the deciduous monsoon forests of river valleys at an altitude of 1000-1400m in Honghe Prefecture, Yunnan Province. It is also distributed in southeastern Yunnan and southwestern Guangxi, and is endemic to southwest my country plant. This species is a characteristic and precious wild woody vegetable plant in the Honghe River Basin of Yunnan Province. It has a long history of utilization. The local people call it "beet". "precious" food. Due to the large market demand and the main dependence on wild resources, resources have been overutilized and habitats have been severely damaged, resulting in a large reduction in wi...

AI Technical Summary

Problems solved by technology

Using the traditional artificial germination method, the emergence rate is less than 30%, and the epicotyl dormancy is serious, and it takes more than one year to grow seedlings

There are data to use the young shoots of the main stem for cutting experiments, and the results show that the survival rate is extremely low. At the same time, young shoots are used as materials, which will cause great damage to the existing wild resources, so the cutting method cannot be promoted.

There is also information that adopts the method of tissue culture to propagate seedlings of sugar beet trees, but basically cannot grow into seedlings.

[0004] Therefore, there is no relatively successful seedling raising method in the prior art to solve the difficulties in beet seedling raising, and how to solve the long-term epicotyl dormancy of beet tree seeds has become the biggest bottleneck in traditional seedling raising

However, no effective method has been found so far