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Preparation method of nano bismuth/graphene composite material and application of nano bismuth/graphene composite material in MALDI-MS

A composite material and nanomaterial technology, applied in the field of preparation of nano-bismuth/graphene composite materials, can solve the problems of uneven crystallization of matrix and sample, increased identification difficulty, poor reproducibility, etc., and achieves overcoming matrix interference and high stability. , the effect of narrow band gap

Active Publication Date: 2021-06-08
广东省农业科学院农业质量标准与监测技术研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining multiple matrices, synthesizing large-molecular-weight matrices, and developing new ionic compound matrices have improved the background interference of the matrix to a certain extent. However, the problems of uneven crystallization between the matrix and the sample, and poor reproducibility have not been completely resolved.
Most of the existing research focuses on the development of various types of inorganic materials for positive ion mode mass spectrometry identification based on the thermally driven dissociation mechanism of nanomaterials. However, a variety of alkali metal ion adducts will be produced in positive ion mode. Complex and difficult to identify

Method used

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  • Preparation method of nano bismuth/graphene composite material and application of nano bismuth/graphene composite material in MALDI-MS
  • Preparation method of nano bismuth/graphene composite material and application of nano bismuth/graphene composite material in MALDI-MS
  • Preparation method of nano bismuth/graphene composite material and application of nano bismuth/graphene composite material in MALDI-MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of nano-bismuth / graphene composite material, comprising the following steps:

[0030] S01. Prepare GO, configure a GO aqueous solution with a concentration of 0.5-1.5 mg / mL;

[0031] S02. Add bismuth salt in step S01, the concentration of bismuth salt is 30mM, stir for 20-40min, and adjust the pH of the solution to be 9-10;

[0032] S03. Place the solution treated in step S02 in a reaction kettle to react at a temperature of 180° C. for 8 to 12 hours;

[0033] S04. Wash the solid obtained in step S03 with ethanol and water for several times, and then vacuum-dry to obtain a bismuth / graphene composite material.

[0034] The bismuth / graphene composite material obtained by the preparation method of the nano-bismuth / graphene composite material.

[0035] Preparation of bismuth oxide nanomaterials: disperse bismuth-containing salts in organic solvents, and obtain required bismuth nanomaterials after high temperature and high pressure solvothermal reactio...

Embodiment 2

[0045]Bismuth oxide nanomaterials and bismuth / graphene composite materials are the same as in Example 1.

[0046] Bismuth-based nanomaterials are used as substrates for matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection. The specific experimental process is as follows:

[0047] 1. Prepare an organic suspension solution of 2.0mg / mL bismuth-based nanomaterials as matrix solution;

[0048] 2. Ultrasonic the above system at room temperature for 1-2 minutes to form a uniform dispersion suspension system;

[0049] 3. Configure 10mM five kinds of mixed amino acid solutions as the sample solution to be tested, the concentration is 10mM, and the specific amino acids are Asp, Glu, His, Arg, and Try.

[0050] 4. Drop the nano-suspension onto the MALDI MS metal target plate, and form a uniform matrix layer after the solution is dried.

[0051] 5. Add the sample solution dropwise to the surface of the dried nanomaterial described in step 3.

[0052] 6...

Embodiment 3

[0055] Bismuth oxide nanomaterials and bismuth / graphene composite materials are the same as in Example 1.

[0056] Bismuth-based nanomaterials are used as substrates for matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection. The specific experimental process is as follows:

[0057] 1. Prepare an organic suspension solution of 2.0mg / mL bismuth-based nanomaterials as matrix solution;

[0058] 2. Ultrasonic the above system at room temperature for 1-2 minutes to form a uniform dispersion suspension system;

[0059] 3. Configure 200mM 5 kinds of mixed fatty acid solutions as the sample solution to be tested, the specific fatty acids are C12:0, C14:0, C16:0, C18:0, C20:0

[0060] 4. Drop the nano-suspension onto the MALDI MS metal target plate, and form a uniform matrix layer after the solution is dried.

[0061] 5. Add the sample solution dropwise to the surface of the dried nanomaterial described in step 3.

[0062] 6. The dried spots were subj...

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Abstract

The invention relates to the technical field of analysis, in particular to a preparation method of a nano bismuth / graphene composite material and the application of the nano bismuth / graphene composite material in MALDI-MS. The preparation method comprises the following steps of S01, preparing GO, and preparing a GO aqueous solution with the concentration being 0.5 mg / mL to 1.5 mg / mL; S02, adding bismuth salt in the step S01, stirring for 20-40 minutes, and adjusting the pH of the solution to be 9-10; S03, putting the solution treated in the step S02 into a reaction kettle to react for 8-12 hours; and S04, cleaning the solid obtained by the reaction in the step S03 with ethanol and water for multiple times, and then carrying out vacuum drying to obtain the bismuth / graphene composite material. According to an MALDI-MS negative ion mode analysis method established based on the bismuth-based nano material, the electron distribution in the system can be influenced by constructing the composite material, so that light absorption of the composite material is increased, separation of electron-hole pairs is accelerated, and the dissociation efficiency of an object to be detected is improved.

Description

technical field [0001] The invention relates to the technical field of analysis, in particular to a preparation method of nano-bismuth / graphene composite material and its application in MALDI-MS. Background technique [0002] Matrix-assisted laser desorption / ionization (MALDI) mass spectrometry has the advantages of high throughput, high sensitivity, high salt tolerance, fast analysis speed, small sample consumption, and simple preparation process. It has become the field of mass spectrometry analysis. One of the widely used technical means. MALDI mass spectrometry has been quite mature in the research of macromolecules such as peptides, proteins, nucleic acids, etc. However, there are still great challenges in the detection of small molecular compounds. Severe matrix background interference, non-uniform matrix coverage and crystallization cause signal instability, poor point-to-point reproducibility, and low ionization efficiency in negative ion mode limit its wide applica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 唐雪妹万凯陈志廷黄健祥高毓文
Owner 广东省农业科学院农业质量标准与监测技术研究所
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