Soybean cell nucleus dissociation liquid suitable for flow cytometry analysis and application thereof
A flow cytometer and cell nucleus technology, applied in the biological field, can solve the problems of non-appearance, low efficiency of separating cell nuclei, unsuitable for large-scale sample preparation and detection, etc., to achieve reliable experimental results, good dissociation effect, and good dissociation effect of effect
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Embodiment 1
[0026] 1 Materials and methods
[0027] 1.1 Experimental materials
[0028] The tested materials are soybean varieties including Williams 82, Holladay, PI 567611, PI 567651, and Fiskeby III, which are preserved by the soybean genetics and breeding laboratory of Yangzhou University.
[0029] 1.2 Method
[0030] 1.2.1 Plant cell nuclei dissociation solution preparation
[0031] The formulations of the six nuclear dissociation solutions used in this experiment are listed in Table 1.
[0032] Table 1 Nuclei Dissociation Buffer Recipe
[0033]
[0034]
[0035] Avoid repeated freezing and thawing of the nuclear dissociation solution, and it can be stored at 4°C for several days after thawing.
[0036] 1.2.2 PEG 6000 simulated drought treatment
[0037] Soybean seeds were planted in the soil after absorbing water and swollen, and germinated and grown for 6 days. Bean sprouts with the same growth state were selected, and the dust was washed under the premise of avoiding da...
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