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Substrate applicable to tissue culture of oncidium and preparation method thereof

A technology of tissue culture and matrix, applied in the field of orchid tissue culture matrix and its preparation, can solve the problems of unfavorable centralized production, poor quality of test-tube seedlings, immature research on the biochemical components of the medium, etc., and achieve robust growth and dark green leaves Effect

Inactive Publication Date: 2017-12-22
贵州安龙金蕙花卉发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the research on oncidium tissue culture at present, such as the immature research on the biochemical components of the culture medium; the quality of the cultured test-tube plantlets; Meet market demand, etc.

Method used

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  • Substrate applicable to tissue culture of oncidium and preparation method thereof
  • Substrate applicable to tissue culture of oncidium and preparation method thereof
  • Substrate applicable to tissue culture of oncidium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A substrate for oncidium tissue culture, including the following raw materials in parts by mass: 80kg of bitter almond, 70kg of Sophora japonica, 60kg of hawthorn, 50kg of moshangcai, 40kg of pickled sorghum leaves, 40kg of Daphne genkwa, 20kg of squid skin, and 15kg of poinciana , Avocado 10kg, oat 10kg, millet 10kg.

[0025] The substrate for Oncidium tissue culture includes the following preparation methods:

[0026] 1) Sophora japonica, hawthorn, and moshangcai were mixed and crushed, and water with twice the mixing quality was added, treated at high temperature and high pressure for 6 minutes, and filtered to obtain filtrate 1;

[0027] 2) Sorghum pickled leaves and skins are mixed and crushed, the mixed powder is mixed with material 1 at a mass ratio of 1:0.7, placed at 60°C for 60 minutes to obtain a reaction material, and 45% ethanol twice the mass of the reaction material is added, extracted at an ultrasonic frequency of 35KHz 70min, filtered to obtain filtrat...

Embodiment 2

[0038] A substrate for oncidium tissue culture, including the following raw materials in parts by mass: 80kg of bitter almond, 70kg of Sophora japonica, 60kg of hawthorn, 50kg of moshangcai, 40kg of pickled sorghum leaves, 40kg of Daphne genkwa, 20kg of squid skin, and 15kg of poinciana , Avocado 10kg, oat 10kg, millet 10kg.

[0039] The substrate for Oncidium tissue culture includes the following preparation methods:

[0040] 1) Sophora japonica, hawthorn, and moshangcai were mixed and crushed, and water with twice the mixing quality was added, treated at high temperature and high pressure for 6 minutes, and filtered to obtain filtrate 1;

[0041] 2) Sorghum pickled leaves and bark were mixed and pulverized, and 45% ethanol twice the mass of the mixed powder was added, extracted at an ultrasonic frequency of 35KHz for 70min, and filtered to obtain filtrate 2;

[0042] 3) Mix and crush genkwa, poinciana, and avocado, add solution 1 of equal mass to the mixed powder, react at ...

Embodiment 3

[0051] A substrate for oncidium tissue culture, including the following raw materials in parts by mass: 80kg of bitter almond, 70kg of Sophora japonica, 60kg of hawthorn, 50kg of moshangcai, 40kg of pickled sorghum leaves, 40kg of Daphne genkwa, 20kg of squid skin, and 15kg of poinciana , Avocado 10kg, oat 10kg, millet 10kg.

[0052] The substrate for Oncidium tissue culture includes the following preparation methods:

[0053] 1) Sophora japonica, hawthorn, and moshangcai were mixed and crushed, and water with twice the mixing quality was added, treated at high temperature and high pressure for 6 minutes, and filtered to obtain filtrate 1;

[0054] 2) Sorghum pickled leaves and skins are mixed and crushed, the mixed powder is mixed with material 1 at a mass ratio of 1:0.7, placed at 60°C for 60 minutes to obtain a reaction material, and 45% ethanol twice the mass of the reaction material is added, extracted at an ultrasonic frequency of 35KHz 70min, filtered to obtain filtrat...

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Abstract

The invention belongs to the field of orchid seedling culture, and particularly relates to a substrate applicable to tissue culture of oncidium. The substrate comprises the following components: bitter almond, flos sophorae, hawthorn, lindernia procumbens, lambert raspberry leaves, lilac daphne flower bud, jasminum giraldii diels, delonix regia, avocado, oat and millet; the substrate added to a culture medium is able to promote the differentiating and the growing of the avocado; the shoot tip starts to germinate after 7 days of grafting during primary culture; clumpy bud points grow from about 50% of explants after 14 days; 3 to 5 strong clumpy buds grow around 86% of explants after 40 days. After the substrate is added to the culture medium, the biochemical components change, and the quality of the cultured seedlings of oncidium is high; the cultured seedlings can grow quickly; the demands of the market on oncidium can be met.

Description

technical field [0001] The invention belongs to the field of orchid seedling cultivation, and in particular relates to a substrate for orchid tissue culture and a preparation method thereof. Background technique [0002] Oncidium, also known as Dancing Orchid, Dancing Girl Orchid, King Phalaenopsis, Tumor Orchid, etc., has 1-3 leaves. The plant is light and slow, the flower stem is light and drooping, and the flowers are strange and lovely. Orchidaceae Oncidium is a general term for plants of the genus Oncidium, which belongs to tropical compound-stemmed orchids, native to the West Indies, Mexico, Brazil and other places. [0003] Oncidium orchids are small and numerous, with changeable shapes, and the flowering period varies with varieties. The flowers can last for 1-3 months. It is a high-quality cut flower material, and it can also be used as a potted plant for people to watch. According to the traditional method of reproduction, an oncidium only reproduces 2-3 buds a ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 刘湘凤
Owner 贵州安龙金蕙花卉发展有限公司
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