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Method for improving proliferation and differentiation of protocorm of Oncidium by utilizing concentrated coconut juice

A technology of oncidium original ball and coconut water, applied in the directions of horticultural methods, botanical equipment and methods, application, etc., can solve the problems of high cost of coconut water, inconvenient transportation, large coconut fruit and the like, and achieves improved reproduction of tissue culture seedlings. rate, increase the proliferation rate, the effect of simple operation

Inactive Publication Date: 2010-07-28
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

[0003] Due to the large amount of coconut water added in tissue culture, and at present, the source of coconut in my country is limited to Hainan Province, the production area is small, and the coconut fruit is large, and the transportation is quite inconvenient. Therefore, the cost of obtaining coconut water in tissue culture factories is relatively high. It is unfavorable for the application of coconut water in tissue culture, and restricts the production route of oncidium tissue culture, but the impact of concentrated coconut water on plant tissue culture process has not been reported yet

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  • Method for improving proliferation and differentiation of protocorm of Oncidium by utilizing concentrated coconut juice
  • Method for improving proliferation and differentiation of protocorm of Oncidium by utilizing concentrated coconut juice

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Embodiment 1

[0027] 1. Material: Oncidium concolor of the same color

[0028] 2. Disinfection of explants: In sunny weather, wrap the stem tip of Oncidium in the morning, take it back to the laboratory and rinse it with tap water quickly, leave the young shoots about 2-3cm long, and use sterile water on the ultra-clean workbench Rinse twice, then soak in 70% alcohol for 1 minute, pour off the alcohol and add 0.15% HgCl 2 The solution was sterilized for 8 minutes, rinsed with sterile water for 5 times, blotted dry with sterilized filter paper, stripped the shoot tip and inoculated it on the induction medium for induction culture, the induction medium was MS+6-BA 4mg / L+ NAA 0.5mg / L+30g / L sucrose+5.0g / L agar powder+1g / L activated carbon+1% volume coconut water; culture conditions are temperature 26°C, pH 5.8, light intensity 1500-2000lx, light 12 hours / day . After 15 days, the shoot tip began to swell, and after 23 days, a callus-like papillae appeared at the base of the shoot tip, and prot...

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Abstract

The invention discloses a method for improving proliferation and differentiation of protocorm of Oncidium by utilizing concentrated coconut juice, which comprises the following steps: concentrating coconut juice on a rotary evaporator to obtain the concentrated coconut juice for later use; sterilizing shoot tip of an Oncidium plant, and inoculating the sterilized shoot tip to an induction medium added with the concentrated coconut juice for induction culture to generate the protocorm; transferring the protocorm to an enrichment medium for enrichment culture, and then transferring to a differential medium for differential culture to generate cluster buds; and inoculating the cluster buds to a rooting and sprouting medium and culturing the cluster buds into seedlings. The concentrated coconut juice serving as an organic additive can obviously improve the proliferation rate of the protocorm tissue of the Oncidium; and on the basis of improving the proliferation rate of the protocorm tissue, the proliferation rate of tissue culture plantlets is effectively improved; therefore, the invention provides an effective path for industrial production of the Oncidium, and has the advantages of strong practicability, simple operation and good popularization.

Description

technical field [0001] The invention relates to a protocorm proliferation and differentiation method of Oncidium orchid, in particular to a method for significantly improving the protocorm proliferation and differentiation of Oncidium by adding concentrated coconut water. Background technique [0002] Orchid is a kind of flower famous for its fragrance. With its unique leaves, flowers and fragrance, it has four cleans (clear air, clear color, clear spirit and clear rhyme) to give people a very pure and elegant image. It has a very high price and is known as a gentleman among flowers. The germination rate of orchid seeds is low, the time is long, and it is not neat, so that the cultivation and production of orchids cannot better meet the needs of the market. At present, the production of orchid seedlings mainly utilizes the technical method of tissue culture, which can effectively improve the rapid propagation coefficient of orchid varieties. Using the method of tissue cult...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄丽云范海阔周焕起李杰覃伟权马子龙
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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