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A kind of oncidium chemical disinfection tissue culture method

An oncidium chemistry and tissue culture technology, applied in the biological field, can solve the problems of high manual operation cost and high energy consumption, and achieve the effect of reducing tissue culture costs, simplifying tissue culture procedures, reducing workload and energy consumption

Inactive Publication Date: 2017-08-08
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The conventional oncidium tissue culture method requires the explants to be inoculated in the sterile medium after high temperature and high pressure sterilization to grow normally, which consumes a lot of energy and high manual operation costs

Method used

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  • A kind of oncidium chemical disinfection tissue culture method
  • A kind of oncidium chemical disinfection tissue culture method

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Embodiment 1

[0038] Embodiment 1: A kind of oncidium chemical disinfection tissue culture method

[0039] A chemical disinfection tissue culture method of Oncidium, comprising the following steps:

[0040] 1. Disinfection of the culture container: Soak the cap of the culture bottle and the stainless steel plate used for inoculation in an aqueous solution of 100mg / L sodium hypochlorite + 10mg / L nisin + 100mg / L calcium propionate for 10 hours, and save it for later use;

[0041]2. Medium preparation: induction medium is MS+4mg / L 6-BA+1.0mg / L NAA+70mg / L sodium hypochlorite+5mg / L nisin+50mg / L calcium propionate+20g / L sucrose+ 3.0g / L agar powder, pH5.8; proliferation medium: MS+3mg / L 6-BA+1.0mg / L NAA+60mg / L sodium hypochlorite+5mg / L nisin+50mg / L calcium propionate +20g / L sucrose+3.0g / L agar powder, pH 5.8; strong seedling medium is MS+0.5mg / L 6-BA+1.0mg / L NAA+60mg / L sodium hypochlorite+5mg / L nisin +50mg / L calcium propionate+20g / L sucrose+3.0g / L agar powder, pH5.8; rooting medium is 1 / 2MS+1.0m...

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Abstract

The invention relates to a chemical disinfection tissue culture method of oncidium. The oncidium chemically sterilized tissue culture method includes culture vessel disinfection, medium preparation, induction culture, proliferation culture, rooting culture and bottle seedling transplantation. The oncidium chemical disinfection tissue culture method of the present invention uses chemical reagents to achieve the purpose of sterilization or bacteriostasis. In the process of preparing the culture medium, the culture container and the culture medium do not need to be sterilized by high temperature and high pressure, which reduces the workload and energy. consumption, which simplifies the oncidium tissue culture link; and the operation is simple, as long as it is prepared according to different medium formulas; it is practical and popular, and can effectively reduce the cost of oncidium tissue culture, generally can reduce the cost More than 10%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a chemically sterilized tissue culture method for oncidium, belonging to the field of biotechnology. Background technique [0002] Oncidium belongs to Orchidaceae Oncidium, also known as Oncidium, which has more than 400 native species. It has small and numerous flowers, bright and colorful colors, and various shapes. The flowers bloom continuously and last for 1 to 3 months. It is a superior potted ornamental and cut flower material. Oncidium's traditional ramets reproduce slowly, with only 2 to 3 buds a year, and the reproduction coefficient is low. Through tissue culture technology, the reproduction speed can be accelerated and factory production can be carried out. Practice has shown that oncidium is harmed by viral diseases in production, resulting in a decline in quality and yield, loss of commodity value, and quality degradation. The rapid propagation of oncidium seedling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭晋隆林庆良许莉萍高世武阙友雄
Owner FUJIAN AGRI & FORESTRY UNIV
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