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Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method

A technology of oncidium group and culture medium, which is applied in the field of tissue culture seedling propagation, can solve the problems of low protocorm proliferation rate and low protocorm proliferation rate of oncidium, and achieve the effect of increasing the multiplication factor

Inactive Publication Date: 2010-04-21
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

[0004] To sum up, the medium used to propagate oncidium hybrids through protocorm or protocorm-like approach is VW, improved No. 1, improved KC, N 6 , 1 / 2MS and MS; Most of the used explants are shoot tips of seedling lateral buds and shoot tips of tissue cultured seedlings, a few are flower spikes, stem segments of pedicel with flower buds and stem segments of pseudobulbs, and explants with 100 to 150 flowers Oncidium pedicel bud explants have not been fully utilized; research data show that the proliferation rate of oncidium protocorms is still lower than that of adventitious buds, for example, in N 6 and MS medium, the proliferation rate of adventitious buds is as high as 450%, while the proliferation rate of protocorms is lower, 250% (Peng Xiaoming et al., 2000)

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  • Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method

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Embodiment Construction

[0032] Firstly, the culture medium required for propagating oncidium tissue culture seedlings was prepared, and the culture medium was prepared according to the following formulas according to the conventional culture medium preparation method.

[0033] The medium for callus induction and protocorm differentiation, which consists of:

[0034] Macroelement Composition: KNO 3 , 550mg / L; 140mg / L (NH 4 ) 2 SO 4 ; Ca(NO 3 ) 2 4H 2 O, 236mg / L; MgSO 4 ·7H 2 O, 370mg / L; KH 2 PO 4 , 250mg / L;

[0035] Trace element composition: FeSO 4 ·7H 2 O, 13.9 mg / L; Na 2 EDTA·2H 2 O, 18.65mg / L; KI, 0.83mg / L; H 3 BO 3 , 6.2mg / L; MnSO 4 4H 2 O, 22.3mg / L; ZnSO 4 ·7H 2 O, 8.6 mg / L; Na 2 MoO 4 2H 2 O, 0.25mg / L; CuSO 4 ·5H 2 O, 0.025mg / L; CoCl 2 ·6H 2 O, 0.025mg / L;

[0036] Organic nutrients: inositol, 1000mg / L; niacin, 0.5mg / L; pyridoxine hydrochloride, 0.5mg / L; thiamine hydrochloride, 0.1mg / L; glycine, 2mg / L;

[0037] Other organic nutrients: coconut milk, 150ml / L;

[0038...

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Abstract

The invention discloses a culture medium for tissue culture of pedicel buds of oncidium hybridum and a tissue cultured seedling propagating method. The culture medium is mainly characterized in that: the combination of concentrations of macroelement components in the culture medium does not exist in the conventional culture medium for the tissue culture of the oncidium hybridum. The method for propagating tissue cultured seedlings of the oncidium hybridum comprises the following steps: utilizing the culture medium to induce the pedicel buds to generate callus and differentiation of protocorm; after the protocorm sprouts and tissue cultured seedlings provided with fake corms are formed, transferring to a rooting medium, and inducing to root; transplanting the rooted tissue cultured seedlings into the medium by a conventional tissue cultured seedling transplanting method. By adopting the technical scheme provided by the invention, the pedicel buds of the hybrid variety of the oncidium hybridum are taken as explants, young plants of the oncidium hybridum are quickly propagated in a way of differentiating the protocorm, and the speed of propagating the young plants of the oncidium hybridum is improved.

Description

technical field [0001] The invention relates to a tissue culture seedling (test tube seedling) propagation technology in plant biotechnology, in particular to a culture medium for oncidium tissue culture and a method for tissue culture seedling propagation. technical background [0002] Oncidium is the general term for plants of the genus Oncidium in the family Orchidaceae. There are hundreds of native species of plants in this genus, but only a few species are used for ornamental purposes. Through interspecies or hybridization with neighboring species, many hybrid varieties with high ornamental value and high commercial value have been bred, especially Onc.Aloha Iwanaga, Onc.Swcct Sugar ), Onc.Gower Ramsey, Onc.Kinsei, Onc.Volcano Queen and Onc.Sharab baby "sweetfrance" It is very popular in my country's flower market and is an important part of my country's orchid industry. [0003] The traditional propagation method of oncidium is division or sowing. Due to the low propag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C05G1/00A01G31/00A01N43/90A01N37/10
Inventor 肖尊安
Owner BEIJING NORMAL UNIVERSITY
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